ʻO SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Hoohana Manao
Ke hoʻohana nei kēia pahu i ka ʻenehana RT PCR Real-time (rRT-PCR) no ka ʻike qualitative o SARS-CoV-2 nucleic acids i nā nasopharyngeal a i ʻole oropharyngeal swab samples.
Kumu o ka hoao ana
Hoʻolālā ʻia kēia ʻano kumu mua a me ka ʻimi noiʻi no ka ʻike ʻana i nā kaʻina i mālama ʻia o SARS-CoV-2 (ORF1ab gene a me N gene).Aia i loko o ka pahu kahi Mana Kūloko i hoʻohana ʻia e loiloi i ka maikaʻi o nā mea hoʻohālike.
Hoʻohana ka ʻenehana PCR Real-Time i ka hopena polymerase chain reaction (PCR) no ka hoʻonui ʻana i ka pahuhopu kikoʻī
nā kaʻina a me nā ʻimi kikoʻī kikoʻī no ka ʻike ʻana i ka RNA i hoʻonui ʻia.Hoʻopaʻa inoa ʻia nā probes me
mea hōʻikeʻike fluorescent a me nā mea hoʻonanea.
Nā kikoʻī wikiwiki
◮Nā kikoʻī:48 Rxns/kit, 96 Rxns/kit
◮ ʻano kikoʻī:96.72%
ʻIkepili:97.92%
◮ʻAno hoʻohālike pili
Nasopharyngeal Swab or Oropharyngeal Swab
◮Mea hana pili
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Nā ʻāpana
ʻAʻole. | ʻāpana | Ka nui | Nā mea nui | |||
48 Rxns | 96 Rxns | |||||
1 | Mea hoʻokuʻu waika nucleic | 1.4mL/ipu | 2 paipu | 5.3 mL/'ōmole | 1 omole | Mea hoʻokuʻu |
2 | RNA pale | 27 μL / paipu | 1 paipu | 53 μL / paipu | 1 paipu | ʻO ka mea hoʻopaneʻe RNase |
3 | ʻO ka hoʻonā hopena SARS-CoV-2 | 800 μL / paipu | 1 paipu | 1600 μL / paipu | 1 paipu | Primer, probe, hoʻopaʻa pane, dNTP |
4 | ʻO ka hui ʻana o ka enzyme SARS-CoV-2 | 80 μL / paipu | 1 paipu | 160 μL / paipu | 1 paipu | Hoʻomaka wela Taq enzyme, M-MLV enzyme |
5 | Ka mana maikaʻi o SARS-CoV-2 | 100 μL / paipu | 1 paipu | 100 μL / paipu | 1 paipu | ʻO ka plasmid recombinant i loaʻa ka ʻāpana pahuhopu, RNA |
6 | ʻO SARS-CoV-2 ka mana ʻino | 1200 μL / paipu | 1 paipu | 1200 μL / paipu | 1 paipu | TE pale |
Nā hiʻohiʻona & pono
◮PCR pololei
ʻAʻohe pono no ka pahu nucleic acid extaction kit a me System, hoʻopau i 96 specimen i 1 hola a i ʻole.
◮Pono nā mea hana haʻahaʻa a me ka hoʻohana maʻalahi
Pono wale ka ʻōnaehana PCR Real-Time.
◮LoD a me ka ʻike kiʻekiʻe
Hiki i ka ʻike ʻike ke piʻi i ka pae haʻahaʻa e like me 500 kope/ml.
Pehea e ho ohana
Hanana 1: Ke ala PCR pololei
Ke Kaʻina 2: Viral RNA Isolation + PCR
Hanana 1: Hoʻohana ʻia ke ʻano PCR pololeiLaʻana Hoʻokuʻu Agena
ʻO ka lawe ʻana a me ka mālama ʻana i nā kaulahao anu
Hoʻopaʻa ʻia mai ka mālamalama a mālama ʻia ma -20±5 ℃;
Ola ola
1 makahiki
ʻIke Kauoha
wikiō hana wikiwiki
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou