ʻO ka Kit Detection NĀ Nucleic SARS-CoV-2 (Multiplex PCR Froorescent Probe Metode)

ʻO ka Kit Detection NĀ Nucleic SARS-CoV-2 (Multiplex PCR Froorescent Probe Metode)

Pahu Wehewehe:

Hoʻohana kēia kime i ka ʻenehana RT PCR manawa maoli (rRT-PCR) no ka ʻike pono ʻana o ka ʻāpana SARS-CoV-2 (ORF1ab a me ka ʻohana N) i loko o nā laʻana swab nasopharyngeal a i ʻole oropharyngeal, e ka multiplex PCR fluorescent probe hana. kahi kaohi kūloko e nānā i ka maikaʻi o ka specimen, no laila ʻaʻole pono no ka hoʻomaʻemaʻe waikawa nukelika, hiki ke hoʻopau i ka hoʻāʻo i ka 1 hola, ke kūpono hoʻi no ka ʻike wikiwiki wikiwiki ʻana.


ʻIke kikoʻī

Nā huahana huahana

Hoakaka

48 Rxns, 96 Rxns

Aʻole.

Mea hana

Huina

Nā mea nui

48 Rxns

96 Rxns

1 Agena hoʻokuʻu hoʻokuʻu waikawa 1.4mL / paipu 2 paipu 5.3 mL / ʻōmole 1 ʻōmole Mea heʻe nalu
2 Palekana RNA 27 μL / paipu 1 paipu 53 μL / paipu 1 paipu RNase mea kāohi
3 ʻO ka hopena hopena SARS-CoV-2 800 μL / paipu 1 paipu 1600 μL / paipu 1 paipu ʻO Primer, ʻimi, buffer reaction, dNTP
4 Hoʻohui fatna SARS-CoV-2 80 μL / paipu 1 paipu 160 μL / paipu 1 paipu Hoʻomaka ka hoʻomaka ʻana o ka enzyme Taq, M-MLV enzyme
5 SARS-CoV-2 mana maikaʻi 100 μL / paipu 1 paipu 100 μL / paipu 1 paipu Hoʻohui hou i ka plasmid i loaʻa i ka ʻāpana target, RNA
6 Kaohi maikaʻi ʻole ʻo SARS-CoV-2 1200 μL / paipu 1 paipu 1200 μL / paipu 1 paipu Buffer TE

Hiʻona & pono

Wikiwiki a maʻalahi hoʻi.
Hōʻalo i ka nalo ʻana o ka acidic acid.
Ka palena haʻahaʻa ʻike a me ka ʻike nui.
Koi pono pono a me ka hoʻohana maʻalahi.

Hiki ke ʻike i ka ʻike i ka pae haʻahaʻa ma ke ʻano he 500 kope / ml).

GCE / mL Ct waiwai
N ʻāpana ʻĀina ORF1ab
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 N / A. N / A.
2.00E-01 N / A. N / A.
NC N / A. N / A.

ʻ .lapa hoʻonui hoʻonui

amplification curve-RT-qPCR

Pehea e ho ohana

1. Mea kani pono

covid-19 nucleic acid detection kit1

2. Ke kaʻina hana ʻike

covid-19 nucleic acid detection kit2

Ka hana ʻana i kahi laʻana: Naopharyngeal a i ʻole oropharyngeal swab hāpana (hana ʻanuʻu 1 wale nō) (kali no 10 mau min)

PCR amplificaton (<= 55min)

Kahua Hana:

covid-19 nucleic acid detection kit4

Waihona

sila ʻia mai ka mālamalama a mālama ʻia ma -20 ± 5 ℃;

storage
storage2

Holopapa ola: 1 makahiki


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