Loaʻa nā hōʻailona DNA i hana ʻia e kā mākou hui e ka enzyme digestion o nā plasmids.ʻO nā māka i hana ʻia e kēia kaʻina hana he maʻemaʻe kāʻei, nā pūʻulu maʻemaʻe, maikaʻi paʻa a hiki ke hoʻokō i ka helu kūpono o nā māka.Aia i loko o ka huahana nā mea pena (cyan dye a me ka melemele), a hiki ke hoʻoholo ʻia ka neʻe ʻana o ka electrophoresis e ka hoʻololi kala i ka wā electrophoresis.ʻO ka neʻe ʻana o ka dye cyan e like me ka 3-5kb i ka 1% agarose gel, a ʻo ka neʻe ʻana o ka wai melemele e pili ana ʻO ka neʻe ʻana e like me ka 50bp band, a hiki ke nānā pono ʻia ka holomua electrophoresis me ka maka ʻōlohelohe.He maʻalahi ke hoʻohana a maopopo ke kiʻi electrophoresis.

He huahana mākaukau kēia huahana a loaʻa iā 1x Loading Buffer.Hiki iā ʻoe ke lawe pololei i kahi nui kūpono o Marker no ka electrophoresis e like me nā pono hoʻokolohua.700bp DNA Marker Ⅰi loko o 7 mau kāʻei DNA, ʻo nā kaula DNA: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (2μl) 700bp(35ng/5μl).