Kiko Hoʻokaʻawale DNA Bacterial Nā pahu hoʻomaʻemaʻe hoʻomaʻemaʻe DNA Genomic DNA
ʻO ka wehewehe ʻana o ka pahu:
E hoʻomaʻemaʻe wikiwiki i ka DNA genomic kiʻekiʻe mai ka bacteria i ka wā ulu logarithmic.
◮ʻAʻole hoʻohaumia ʻo RNase:ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka DNA genomic me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
◮wikiwiki wikiwiki:ʻOi aku ka hana o Foregene Protease ma mua o nā protease like, a ʻeli koke i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 20-80 mau minuke.
◮ʻoluʻolu:Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.
◮Palekana:ʻAʻole koi ʻia ka hoʻoheheʻe ʻana i nā mea hoʻoheheʻe organik.
◮kūlana kiʻekiʻe:ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
◮Pūnaehana micro-elution:Hiki iā ia ke hoʻonui i ka manaʻo o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.
wehewehe
Hāʻawi kēia pahu i kahi ala wikiwiki a maʻalahi o ka unuhi ʻana i ka DNA genomic mai nā moʻomeheu bacteria (Gram-negative a me Gram-positive) mai nā kumu like ʻole;hiki iā ia ke hana ma lalo o 3ml o nā moʻomeheu bacteria i ka manawa ulu logarithmic (1×109 bacteria).Hoʻohālikelike ʻia ka pahu me ka Foregene Protease kiʻekiʻe, e hiki ai ke kiʻi ʻia ka 15-50μg o ka DNA genomic kiʻekiʻe i loko o 1 hola.Eia kekahi, hiki i ka kit ke unuhi i nā mea genetic ʻē aʻe ma mua o ka genome, e like me plasmids, Cosmid, BAC, etc.
ʻO ka DNA-wale silica gel matrix material i hoʻohana ʻia ma ke kolamu spin he mea hou kū hoʻokahi o ka hui, hiki iā ia ke hoʻopili pono i ka DNA.ʻO 80μg ka nui o ka adsorption no ka DNA.Hiki i ka ʻōnaehana kū hoʻokahi a me ka elution ke hoʻonui i ka wehe ʻana i ka RNA, nā protein impurity, nā ion a me nā mea hoʻohui ʻē aʻe i loko o nā cell.ʻO ka'āpana DNA genomic i unuhiʻia he nui, maʻemaʻe kiʻekiʻe, kūpaʻa a hilinaʻi maikaʻi, a paʻa ka nui o ka'āpana DNA ma kahi o 23kb.
Nā kikoʻī
50 Hoʻomākaukau, 100 Hoʻomākaukau, 250 Hoʻomākaukau
Nā ʻāpana pahu
| Hoʻopaʻa ML1 |
| Hoʻopaʻa ML2 |
| Hoopaa PW |
| Hoʻopaʻa WB |
| Hoʻopaʻa EB |
| Foregene Protease |
| Lysozyme |
| Hoʻopaʻa TE |
| DNA-wale wale ke kolamu |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
-ʻAʻohe RNase contamination: ʻAʻole pono e hoʻohui i ka RNase e wehe i ka RNA i loko o ka DNA genomic, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
ʻO ka wikiwiki wikiwiki: He ʻoi aku ka ʻoihana o Foregene Protease ma mua o nā protease like a hiki ke ʻeli i nā laʻana bacteria i haki i ka manawa pōkole.
-ʻoluʻolu: Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.
- Palekana: ʻaʻole koi ʻia ka hoʻoiho reagent organik.
-Kiʻekiʻe kiʻekiʻe: ʻO nā ʻāpana DNA genomic i unuhi ʻia he nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
Ke noi pahu
He mea kūpono kēia pahu no ka hoʻomaʻemaʻe ʻana i ka DNA genomic o kēia mau laʻana: Gram-negative bacteria a me Gram-positive bacteria i loko o ka logarithmic growth phase.
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
-Hiki ke mālama ʻia kēia kit no 12 mau mahina ma lalo o nā kūlana maloʻo ma ka lumi wela (15-25 ° C);inā pono e mālama ʻia no ka manawa lōʻihi, hiki ke mālama ʻia ma 2-8 ° C.
'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hoʻokomo i ka hoʻonā i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa i ka wai ʻauʻau 37 ° C no 10 mau minuke e hoʻoheheʻe i ka wai, a hui ʻia ma mua o ka hoʻohana ʻana.
-Foregene Protease solution he kumu kū hoʻokahi, e hana ana i ka wā e mālama ʻia ai i ka lumi wela no ka manawa lōʻihi (3 mau mahina);ke mālama ʻia ma 4 ° C, ʻoi aku ka maikaʻi o kāna hana a me ka paʻa, no laila e ʻōlelo ʻia e mālama iā 4 ° C, e hoʻomanaʻo ʻaʻole e mālama iā -20 ° C.
-Lysozyme kahi pauka maloʻo, e hana ana ke mālama ʻia ma 2-8 ° C no ka manawa lōʻihi (3 mau mahina).No ka mālama lōʻihi, e ʻoluʻolu e mālama iā ia ma -20°C.
