Koko DNA Midi Kit (1-5ml) Koko Isolation Midi Kits no DNA mai Koko
ʻO ka wehewehe ʻana o ka pahu:
E hoʻomaʻemaʻe koke i ka DNA genomic kiʻekiʻe mai ke koko anticoagulated (1-5ml).
◮ʻAʻole hoʻohaumia ʻo RNase:ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka DNA genomic me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
◮wikiwiki wikiwiki:ʻOi aku ka hana o Foregene Protease ma mua o nā protease like, a ʻeli koke i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 20-80 mau minuke.
◮ʻoluʻolu:Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.
◮Palekana:ʻAʻole koi ʻia ka hoʻoheheʻe ʻana i nā mea hoʻoheheʻe organik.
◮kūlana kiʻekiʻe:ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
◮Pūnaehana micro-elution:Hiki iā ia ke hoʻonui i ka manaʻo o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.
wehewehe
Aia i loko o ka hāpana koko ka nui o nā keʻena koko ʻulaʻula a me nā ʻāpana platelet, kahi e hoʻopilikia ai i ka hana koko.Eia naʻe, ʻaʻole kiʻekiʻe ka maʻemaʻe o ka DNA genomic koko i unuhi ʻia e ke ʻano maʻamau a haʻahaʻa loa ka ʻike.Ke hoʻohana nei kēia pahu i ka Foregene Protease Plus hou a me nā ʻōnaehana pale BL1 a me BL2 kūʻokoʻa, hiki ke hoʻokaʻawale loa i ke koko anticoagulated acoagulationnā laʻana i ka manawa pōkole loa, no laila e pale aku i ka hōʻino ʻana o DNA i ka nui loa a loaʻa ka nui o ka DNA genomic.ʻO ka ʻōnaehana hoʻoheheʻe koko wikiwiki a me ka hana spin column maʻalahi e maʻalahi i ka unuhi ʻana o ka genome koko, i hiki ke loaʻa ka DNA genomic kiʻekiʻe, maʻemaʻe kiʻekiʻe i loko o 40 mau minuke.
ʻO ka DNA-wale silica gel membrane i hoʻohana ʻia i loko o ke kolamu spin o kēia kit he mea ʻokoʻa hou ia o ka hui, kahi e hoʻopili pono ai i ka DNA, a hiki ke hoʻopau i ka RNA, nā protein haumia, nā ion a me nā mea hoʻohui ʻē aʻe i loko o nā cell a hiki i ka nui loa.Nui nā ʻāpana DNA i loaʻa, me ka maʻemaʻe kiʻekiʻe, kūpaʻa a hilinaʻi maikaʻi, a ʻo ka nui o ka lawe ʻana o kahi kolamu spin he 80 μg DNA.Hiki ke hoʻohana ʻia ka DNA i loaʻa no nā hoʻokolohua molecular biology e like me ka restriction digestion, PCR, Southern hybridization, kūkulu hale waihona a pēlā aku.Hiki i ke kit ke hana i ka 5ml o ke koko i ka manawa e loaʻa ai ka nui o ka DNA genomic maʻemaʻe kiʻekiʻe.
Nā kikoʻī
50 Hoʻomākaukau, 100 Hoʻomākaukau, 250 Hoʻomākaukau
Nā ʻāpana pahu
| Hoʻopaʻa BL1 |
| Hoʻopaʻa BL2* |
| Kākoʻo DC |
| Hoʻopaʻa PW* |
| Mālama WB1 |
| Hoʻopaʻa EB |
| Foregene Protease Plus |
| DNA-wale wale ke kolamu |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
-ʻAʻohe RNase contamination: ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka genomic DNA me ka ʻole o ka hoʻohui ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
ʻO ka wikiwiki wikiwiki: ʻoi aku ka ʻoi aku o ka ʻoihana Foregene Protease Plus ma mua o nā protease like, a e hoʻoheheʻe koke ʻo ia i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 40 mau minuke.
-Maikaʻi: Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no 4°C centrifugation haʻahaʻa-maha a i ʻole ethanol precipitation o DNA.
- Palekana: ʻAʻole koi ʻia ka hoʻoheheʻe reagent organik.
-Kiʻekiʻe kiʻekiʻe: ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
Ke noi pahu
He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA genomic mai ke koko holoʻokoʻa anticoagulated hou a maloʻo paha.
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
-Hiki ke mālama ʻia kēia kit no 12 mau mahina ma lalo o nā kūlana maloʻo ma ke ana wela (15-25℃);inā pono e mālamaʻia no ka manawa lōʻihi, hiki ke mālamaʻia ma 2-8℃.
'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hōʻoia e waiho i ka hopena i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa i ka wai ʻauʻau 37 ° C no 10 mau minuke e hoʻoheheʻe i ka wai, a hui ʻia ma mua o ka hoʻohana ʻana.
-Foregene Protease Plus solution he kumu kū hoʻokahi, e hana ana i ka wā e mālama ʻia ai i ka lumi lumi no ka manawa lōʻihi (3 mau mahina), ʻoi aku ka maikaʻi o kāna hana a me ka paʻa i ka mālama ʻia ʻana ma 4 ° C, no laila ua ʻōlelo ʻia e mālama iā ia ma 4 ° C, e hoʻomanaʻo ʻaʻole e mālama iā ia ma -20℃.
