Koko SuperDirectᵀᴹ PCR Kit-EDTA Koko Direct PCR Master Mix no ka Genotyping o ke koko
Nā kikoʻī
200 ×20μl rxns, 2000 ×20μl rxns
2× MāmāTMMix-EDTA: Loaʻa i ka D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc.2O i ka 2× SuperEasyTMHoʻohui-EDTA e hoʻohana ʻia i ka hopena PCR.
6× DNA Loading Buffer: ʻAʻohe SDS i ka Loading Buffer.Manaʻo ʻia e hoʻohana i ka 6 × DNA Loading Buffer i hoʻokomo ʻia me ka pahu i ka wā e hana ai i ka electrophoresis gel agarose i mea e loaʻa ai nā hopena electrophoresis maikaʻi.Mai hoʻohana i ka Loading Buffer i loaʻa iā SDS, i ʻole e loaʻa kahi huelo nui o ke kukui ʻālohilohi i ke ala i ka wā electrophoresis, e pili ana i nā hopena hoʻokolohua.
Nā ʻāpana pahu
2× MāmāTMHui-EDTA |
6× DNA Hoʻouka Pahu |
Nā kuhikuhi |
Nā hiʻohiʻona & pono
-ʻAʻohe pono no ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi, ʻaʻohe pretreatment, a me ka hoʻohana pololei ʻana i ke koko holoʻokoʻa ma ke ʻano he PCR template.
-He mana hoʻonui ikaika ka ʻōnaehana a hiki ke ʻike maʻalahi i ka genome a me nā ʻāpana DNA target haole i loko o ke koko.
-2× MāmāTMMix-EDTA Ka hoʻomanawanui koko kiʻekiʻe: a hiki i ka 45% i ke koko kanaka a me 20% i ke koko ʻiole.
- Hoʻopili piha ʻia ka laʻana, no laila ʻaʻohe pono e hopohopo e pili ana i ka hoʻohaumia ʻana o ka laʻana a me nā hopena PCR maikaʻi ʻole.
Ke noi pahu
Hoʻolaʻa ʻia i ka ʻike PCR pololei o ke koko holoʻokoʻa anticoagulated EDTA.(E like me: koko kanaka, koko ʻiole, koko moa, koko manu, koko bipi, koko ʻīlio, etc.)
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
Pono e mālama ʻia kēia pahu ma kahi -20 ° C.
2× MāmāTMHoʻohui-EDTA, inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4 ℃ no ka mālama pōkole (hoʻohana i loko o 10 mau lā).
6 × DNA Loading Buffer, hiki ke mālama ʻia ma 4 ° C a i ʻole -20 ° C no ka manawa lōʻihi.