Koko SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Koko Direct PCR Master Mix no ka Genotyping o ke koko
ʻO ka wehewehe ʻana o ka pahu:
E hoʻohana pololei i ke koko holoʻokoʻa heparin anticoagulated ma ke ʻano he kumu hoʻohālike no ka hoʻonui ʻana i ka PCR, me ka naʻau kiʻekiʻe a me ka pono hoʻonui kiʻekiʻe.Ua hoʻohui ʻia ka ʻōnaehana anti-pollution UNG e pale i nā hopena maikaʻi ʻole.
◮Loaʻa i ka ʻōnaehana ka mana amplification ikaika a hiki ke ʻike maʻalahi i ka genome a me nā ʻāpana DNA target haole i loko o ke koko.
◮2× MāmāTMHoʻohui (UNG)-Heparin Ka hoʻomanawanui koko kiʻekiʻe: a hiki i 45% i ke koko kanaka a me 20% i ke koko ʻiole.
◮Hoʻopili piha ʻia ka hāpana, no laila ʻaʻohe pono e hopohopo e pili ana i ka hoʻohaumia ʻana o ka laʻana a me nā hopena PCR maikaʻi ʻole.Pūnaehana PCR anti-pollution
◮2×SuperEasyTMHoʻohui (UNG), hoʻopau pono i ka pollution i hoʻokumu ʻia e nā huahana PCR, a hōʻoia i ka kikoʻī a me ka pololei o ka amplification.
Nā kikoʻī
200 ×20μl rxns, 2000 ×20μl rxns
2× MāmāTMHoʻohui (UNG) -Heparin: Ma ke kumu o 2 × SuperEasyTMHoʻohana ʻia ʻo Mix-Heparin, dUTP ma kahi o ka dTTP, a ua hoʻohui ʻia ka enzyme UNG e hiki ke hoʻohaʻahaʻa i ka template dUTP i ka manawa like, hiki ke pale pono i ka hoʻohaumia ʻana o nā huahana PCR amplification.Aia ka D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc.2O i ka 2× SuperEasyTM Mix(UNG)-Heparin e hoʻohana ʻia i ka hopena PCR.
6× DNA Loading Buffer: ʻAʻohe SDS i ka Loading Buffer.Manaʻo ʻia e hoʻohana i ka 6 × DNA Loading Buffer i hoʻokomo ʻia me ka pahu i ka wā e hana ai i ka electrophoresis gel agarose i mea e loaʻa ai nā hopena electrophoresis maikaʻi.Mai hoʻohana i ka Loading Buffer i loaʻa iā SDS, i ʻole e loaʻa kahi huelo nui o ke kukui ʻālohilohi i ke ala i ka wā electrophoresis, e pili ana i nā hopena hoʻokolohua.
Nā ʻāpana pahu
| 2× MāmāTMHui (UNG)-Heparin |
| 6× DNA Hoʻouka Pahu |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
-Loaʻa i ka ʻōnaehana ka mana amplification ikaika a hiki ke ʻike maʻalahi i ka genome a me nā ʻāpana DNA target haole i loko o ke koko.
-2× MāmāTMHoʻohui (UNG)-Heparin Ka hoʻomanawanui koko kiʻekiʻe: a hiki i 45% i ke koko kanaka a me 20% i ke koko ʻiole.
- Hoʻopili piha ʻia ka laʻana, no laila ʻaʻohe pono e hopohopo e pili ana i ka hoʻohaumia ʻana o ka laʻana a me nā hopena PCR maikaʻi ʻole.Pūnaehana PCR anti-pollution 2×SuperEasyTMHoʻohui (UNG), hoʻopau pono i ka pollution i hoʻokumu ʻia e nā huahana PCR, a hōʻoia i ka kikoʻī a me ka pololei o ka amplification.
Ke noi pahu
Hoʻolaʻa ʻia i ka ʻike PCR pololei o ke koko holoʻokoʻa heparin anticoagulated.(E like me: koko kanaka, koko ʻiole, koko moa, koko manu, koko bipi, koko ʻīlio, etc.)
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
ʻO ke kaʻina holoʻokoʻa o ka lawe ʻana i ka pahu hau haʻahaʻa haʻahaʻa, e hōʻoia i ka pahu i loko o ke kūlana <4 ℃.
2× MāmāTMHoʻohui (UNG) -Heparin, inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4 ° C no ka mālama pōkole (hoʻohana i loko o 10 mau lā).
6 × DNA Loading Buffer, hiki ke mālama ʻia ma 4 ° C a i ʻole -20 ° C no ka manawa lōʻihi.
