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Pūnaewele Direct RT qPCR
Hoʻohana kēia kime i kahi ʻōnaehana lysis buffer kūikawā hiki ke hoʻokuʻu koke iā RNA mai nā laʻana pūnaewele moʻomeheu no nā hopena RT-qPCR, a laila hoʻopau i ke kaʻina hana hoʻomaʻemaʻe RNA hoʻolōʻihi. Hiki ke loaʻa ke anakuhi RNA i loko o 7 mau minuke wale nō. ʻO ka 5 × Direct RT Mix a me 2 × Direct qPCR Mix-SYBR reagents i hāʻawi ʻia e ka kit hiki ke loaʻa me ka wikiwiki a me ka maikaʻi i nā hopena PCR nui maoli manawa maoli.
5 × Direct RT Mix a me 2 × Direct qPCR Mix-SYBR i ke ahonui inhibitor ikaika, a hiki ke hoʻohana ʻia ka lysate o nā laʻana e like me ke anakuhi no RT-qPCR pololei. Aia i loko o kēia pahu ka RNA kiʻekiʻe-loa Affinity Foregene huli transcriptase, a me Hot D-Taq DNA polymerase, dNTPs, MgCl2, buffer pane, PCR optimizer a me stabilizer.
