ForeSNP Genotyping Kit
ʻO ka wehewehe ʻana o ka pahu:
ʻO ka pololei kiʻekiʻe: ke paʻi ʻana i ka mana maikaʻi, ʻoi aku ka nui o ka pololei ma luna o 98%.
Ke kumu kūʻai haʻahaʻa: ʻAʻole pono e synthesize i kahi helu nui o nā mea hoʻopaʻa inoa pālua.
Pūnaehana PCR maikaʻi: kūpaʻa ʻōnaehana maikaʻi a me ka kikoʻī hoʻonui ikaika.
Pūnaehana PCR Anti-pollution: Hiki iā ia ke hoʻopau pono i ka pollution aerosol i hoʻokumu ʻia e nā huahana PCR, me ka hopohopo ʻole e pili ana i ka hoʻopili ʻana o ka pollution kaiapuni ma ka hōʻailona fluorescent o ka mana maikaʻi ʻole, a hōʻoia i ka kikoʻī o ka amplification a me ka pololei o ka paʻi ʻana.
Nā kikoʻī
ʻO ka ʻenehana Competitive Allele Specific PCR (Competitive Allele Specific PCR) ʻenehana he ʻano hou o ke ʻano paʻi allele.ʻAʻole pono kēia ʻano hana e synthesize i nā ʻimi kikoʻī no kēlā me kēia SNP a me inDel, akā pono ʻelua mau pālua o nā ʻimi kūʻokoʻa kūʻokoʻa no ka hoʻokō pololei ʻana i nā hōʻailona DNA genomic.Ma ke kālailai ʻana i ka ikaika a me ka ratio o ka hōʻailona fluorescence hope, hoʻoholo ʻokoʻa ka genotype, a hōʻike ʻia ka hopena clustering.He manawa pōkole kēia ʻano, ke kumu kūʻai haʻahaʻa haʻahaʻa, ka pololei ʻike kiʻekiʻe, a hiki ke hoʻohana ʻia no ka molecular marker-assisted breeding, QTL positioning, genetic marker identification and other molecular biology experiments with large sample volume.
Nā kikoʻī
5ml, 50ml, 50ml×10, 500ml×20
Nā ʻāpana pahu
| 2× GT maʻalahiTMHuikau |
| DNase-Free ddH2O |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
■ʻO ka pololei kiʻekiʻe: ke paʻi ʻana i ka mana maikaʻi, ʻoi aku ka nui o ka pololei ma luna o 98%.
■ Ke kumu kūʻai haʻahaʻa: ʻAʻole pono e hoʻohui i kahi helu nui o nā mea hoʻopaʻa inoa pālua.
■ Pūnaehana PCR maikaʻi: kūpaʻa ʻōnaehana maikaʻi a me ka kikoʻī hoʻonui ikaika.
■ Anti-pollution PCR nenoaiu: Hiki ke hoopau pono i ka pollution aerosol i hanaia e na huahana PCR, me ka hopohopo ole e pili ana i ke keakea ana o ka pollution kaiapuni ma ka hoailona fluorescent o ka mana ino, a e hooiaio i ka kikoo o ka amplification a me ka pololei o ke kikokiko.
Mea hoʻohana kit
Manaʻo ʻia no ka hoʻohana ʻana i ka hoʻāʻo genotyping o nā laʻana DNA i hoʻomaʻemaʻe ʻia.
Laʻana
Ma kēia hoʻokolohua, ua hoʻohana ʻia ʻo 200ng wheat genomic DNA ma ke ʻano he laʻana no ka ʻike ʻana i ka paʻi ʻana o ka gene TaGS-D1 e pili ana i ke kaumaha o ka palaoa ma lalo o ka Foresnp Genotyping Kit.ʻO ke kumu hoʻohālike ʻo BIO-RAD CFX hoʻohui;ʻO ka helu o nā laʻana he 21, ʻo ka helu o nā NTC he 8, a ʻo kēlā me kēia ʻano o ka mana maikaʻi he 1.
ForeSNP Genotyping Kit
