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Lnc-RT Heroᵀᴹ I (Me gDNase) (Super Premix no ka syntand cDNA pae mua mai ka lncRNA)
Lnc-RT LaniTM ʻO I (Me gDNase) kahi ʻōnaehana transcription hoʻohuli i hoʻomohala kūikawā ʻia no ka lncRNA e hoʻopau koke i ka genomic DNA haumia. Hiki iā 5 × gDNase Mix ke wehe koke i ke koena o ka genome ma RNA ma 42 ° C no 2 mau minuke, me ka pale pono ʻana i ka genome o nā genome ma nā hopena qPCR.
5 × L-RT LaniTM Loaʻa i loko o Mix ka Foregene LncRNA Reverse Transcriptase i hoʻomohala kūikawā ʻia e Foregene, kahi ʻano hou o ka transcriptase hope kikoʻī no lncRNA a me nā ʻōkuhi RNA paʻakikī ʻē aʻe, me ka pilina RNA ʻoi aku ka ikaika a me ka ʻoi aku ka maikaʻi o ka hoʻopaʻa ʻana i ka leo. ʻO ka ʻōnaehana i hoʻomākaukau ʻia e hoʻolalelale ai i ka helu transcription huli a hiki maʻalahi ke kākau i nā anakuhi RNA me nā ʻike GC kiʻekiʻe a me nā hanana lua paʻakikī. Hiki ke hoʻopau ʻia ka syntandy cDNA mua ma 42 ° C i 15 mau minuke.
