ʻO China Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Mouse Tail) (no Genotyping) Mea hana a mea hoʻolako |Foregene

Kiko PCR pololei huelo iole-UNG Direct PCR Lysis Reagent(Huo Iole)(no ka Genotyping)

Kiʻi PCR Direct Tail-UNG Direct PCR Lysis Reagent(Huo Iole)(no ka Genotyping) Kiʻi Hōʻikeʻike

ʻO ka wehewehe ʻana o ka pahu:

Hoʻohana pololei ʻia ka lysate huelo ʻiole ma ke ʻano he laʻana no ka hoʻonui ʻana i ka PCR me ka ʻole o ka unuhi ʻokoʻa ʻana o DNA, he wikiwiki a paʻakikī loa.Hoʻohui ʻia ʻo UNG anti-pollution system e hoʻopau i nā hopena maikaʻi ʻole.

◮ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.

◮He liʻiliʻi ke koi hāpana, lawe wale i hoʻokahi hua.

◮ʻAʻole koi ʻia nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

◮ʻO ka ʻōnaehana PCR i hoʻopaʻa ʻia e hiki ai i ka PCR ke loaʻa ka kikoʻī kiʻekiʻe aʻe a ʻoi aku ka ikaika o ka hoʻomanawanui i ka PCR reaction inhibitors.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Nā wehewehe

Ke hoʻohana nei kēia pahu i kahi ʻōnaehana lysis buffer kūʻokoʻa e hoʻokuʻu koke i ka DNA genomic mai nā huelo ʻiole, nā pepeiao, nā ʻiʻo a me nā ʻano kikoʻī ʻē aʻe no ka hopena PCR, no laila ua kūpono loa ia no ka hoʻāʻo ʻana o ka genetic nui.

Hoʻopau ʻia ke kaʻina hana o ka hoʻokuʻu ʻana i ka DNA genomic mai ka lysis buffer i loko o 10-30 mau minuke ma 65 ° C.ʻAʻole koi ʻia nā kaʻina hana ʻē aʻe e like me ka protein a me ka wehe ʻana i ka RNA, a hiki ke hoʻohana ʻia ka trace DNA i hoʻokuʻu ʻia ma ke ʻano he kumu hoʻohālike no ka hopena PCR.

2× M1-PCR maʻalahi^TMHoʻohana ʻo Mix (UNG) i ka dUTP ma kahi o ka dTTP ma ke kumu o 2× M1-PCR Easy^TMHoʻohui (UNG), a hoʻohui i ka enzyme UNG (Uracil-N-glycosylase) hiki ke hoʻohaʻahaʻa i ke kumu hoʻohālike i loaʻa ka dUTP i ka manawa like.Ma mua o ka hopena PCR, hoʻohana ʻia ka enzyme UNG e hoʻohaʻahaʻa i ka huahana PCR i loaʻa ka uracil.ʻAʻole loaʻa ka hopena o ka enzyme UNG i ka template i loaʻa ʻole ka uracil, no laila e hōʻoiaʻiʻo ai i ka kikoʻī a me ka pololei o ka amplification a me ka pale ʻana i ka hiki ke hoʻāʻo genetic nui.Ua hoʻopōʻino ʻia nā huahana PCR.

ʻO D-Taq DNA polymerase kahi DNA polymerase i hoʻomohala kūikawā ʻia e Foregene no nā hopena PCR pololei.Hiki i ka D-Taq DNA polymerase ke hoʻomanawanui i ka PCR reaction inhibitors, a hiki ke hoʻonui i ka nui o ka DNA i loko o nā ʻōnaehana hoʻololi paʻakikī, a hiki i ka wikiwiki hoʻonui ke hiki i 2Kb/min, kūpono loa ia no ka pane pololei PCR.

Nā kikoʻī

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nā ʻāpana pahu

Mahele I	Palena MP
	Foregene Protease Plus
	6× DNA Hoʻouka Pahu
Mahele II	2× M1 PCR maʻalahi^TMHui (UNG)
Nā kuhikuhi

Nā hiʻohiʻona & pono

■ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.

■ He liʻiliʻi ke koi hāpana, hiki ke hoʻāʻo ʻia e like me ka 5mg ʻiole a i ʻole 2-5mm huelo ʻiole.

■ ʻAʻole koi ʻia nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

■ Ma muli o ka ʻōnaehana PCR maikaʻi loa, ʻoi aku ka maikaʻi o ka PCR a ʻoi aku ka ikaika o ka hoʻomanawanui ʻana i ka PCR reaction inhibitors.

■ Pūnaehana PCR anti-pollution 2×M1-PCR maʻalahi^TMʻO Mix (UNG), ka mea e hoʻopau pono i ka pollution i hoʻokumu ʻia e nā huahana PCR a hōʻoia i ka kikoʻī a me ka pololei o ka amplification.