ʻO ka hānau ʻana o PCR
PCR (Polymerase Chain Reaction)
Ua ʻoi aku ma mua o 30 mau makahiki mai ka wā i ʻike ʻia ai ka hopena o ke kaulahao polymerase.No nā makahiki he 30 aʻe, ma hope o ka hoʻomau ʻana a me ka hoʻomaikaʻi ʻana o ka nui o nā loea a puni ka honua, ua lilo ka ʻenehana PCR i mea nui loa a hoʻohana pinepine ʻia a ʻoi aku ka nui o ke ʻano noiʻi kumu ma ke kahua holoʻokoʻa Life Science.
Ua hoʻomohala ʻia ka TouchDown PCR, Real-Time PCR, Multi PCR, a me nā mea ʻē aʻe ma ke kumu o ka hoʻohana ākea o ka ʻenehana PCR kuʻuna, a me ka Digital PCR (digital PCR) hou, ua hoʻonui nui i nā ʻano noiʻi o ka hapa nui o nā mea noiʻi ʻepekema a ua wikiwiki loa i ke kaʻina hana o nā ʻepekema Ola hou, ʻoi aku ka molecular biology.
Nā hemahema o ka ʻenehana PCR kuʻuna
ʻO ka hoʻokaʻawale ʻana o ka waika nucleic paʻakikī aunuhi:
★ ʻenehana PCR kuʻuna: pono
★ ʻenehana i loaʻa PCR: pono
★ Nā hōʻailona DNA a me RNA: nā ʻokoʻa nui, nā koi hana paʻakikī
★ Nā pōʻino kino: hoʻopōʻino ke kino i nā mea ʻawaʻawa
Loaʻa i ka ʻenehana PCR kuʻuna a me ka ʻenehana derivative kahi hoʻokaʻawale a me ka hoʻomaʻemaʻe ʻana i ka waika nucleic.
Pono kekahi laʻana olaola e hele i ke ʻano o ka hana laʻana paʻakikī a paʻakikī e loaʻa ai nā laʻana nucleic acid i kūpono i nā koi o ka ʻenehana PCR.
ʻO ka hoʻokaʻawale ʻana a me ka unuhi ʻana o DNA a me RNA he hana maʻamau e pono ai nā mea noiʻi ʻepekema kūpono e hana hou i kēlā me kēia lā.
Ma muli o ka ʻokoʻa nui ma waena o nā laʻana, ʻokoʻa loa ka hoʻokaʻawale ʻana a me nā kaʻina hana o ka DNA a me RNA.Pono kēia hana i kahi kūlana kiʻekiʻe o ka mākaukau ʻenehana no nā mea hana.Pono nā ʻenehana hoʻokaʻawale maʻamau a me ka unuhi ʻana i ka pilina lōʻihi me kekahi mau mea hoʻoheheʻe kemika nui loa.E hoʻopōʻino ʻole ia i ke kino o ka mea hoʻohana, a e hōʻeha pololei i ka wā o ka hoʻokolohua.
I ka manawa like, no ka poʻe i loaʻa ka nui o nā laʻana e aʻo ai, ʻo ka hoʻokaʻawale ʻana a me ka unuhi ʻana o nā waikawa nucleic he hana koʻikoʻi.
ʻO ka hoʻokaʻawale ʻana i ka waika nucleic a me nā pahu hoʻoheheʻe ma ka mākeke i kēia manawa ke oʻo nei a he nui nā hōʻailona, akā ua like like lākou.Inā he silica gel membrane column centrifugal kit a i ʻole ka pahu hana bead magnetic, he nui ka manawa a he kumu kūʻai.Ma waho aʻe o ke kumukūʻai o ka pahu, aia kekahi mau koi kūikawā no nā mea hana hale hana.ʻO ke keʻena hana automated i hoʻohana ʻia i ke ʻano kuʻuna magnetic he mea maʻamau maʻamau nui ʻoi aku ka waiwai kiʻekiʻe, ʻo ia ka uku nui no ka hale hana.
Ma ka hōʻuluʻulu
Ma mua o ka hana ʻana i nā hoʻokolohua PCR, ʻo ka hoʻomaʻamaʻa mua ʻana i nā laʻana he mea hiki ʻole ke pale ʻia a maʻa mau no ka poʻe noiʻi.Pehea e hoʻoponopono ai i kēia pilikia a inā hiki ke hana ʻia nā hoʻokolohua PCR me ka ʻole o ka hoʻokaʻawale ʻana a me ka unuhi ʻana o nā waikawa nucleic i manaʻo mau ʻia e ka hapa nui o nā mea noiʻi ʻepekema a me nā limahana lapaʻau lapaʻau.
ʻO Foregene's Solution
Ma hope o nā makahiki o ka noiʻi ʻana i ka ʻenehana Direct PCR a me nā pahu pili, ua haki ʻo Forgene i nā bottlenecks he nui a loaʻa maikaʻi ka PCR pololei no nā ʻano laʻana like ʻole me ke kūpaʻa ikaika a me ka hoʻololi ʻana, e ʻae ana i nā mea noiʻi e hoʻopau i ka hoʻokaʻawale paʻakikī a me ka pōʻino a me ka unuhi ʻana i nā waikawa nucleic.ʻO kēia ka mea e hōʻemi nui ai i ka ikaika o ka hana a kēlā me kēia kanaka, e wikiwiki i ke kaʻina hana hoʻokolohua, a mālama i nā kumukūʻai noiʻi ʻepekema a hoʻāʻo.
ʻO ka ʻike a me ka ʻike o Forgene no DirectPCR
ʻO ka mua, ʻo ka ʻenehana DirectPCR kahi ʻenehana PCR pololei no nā ʻiʻo laʻana olaola like ʻole.Ma lalo o kēia kūlana ʻenehana, ʻaʻohe pono e hoʻokaʻawale a wehe i nā ʻakika nucleic, a hoʻohana pololei ʻia ka ʻiʻo kikoʻī ma ke ʻano he mea, a hoʻohui ʻia nā kumu kumu kumu no ka hopena PCR.
ʻO ka lua, ʻo ka ʻenehana DirectPCR ʻaʻole ia he ʻenehana hoʻonui hoʻohālikelike DNA kuʻuna, akā loaʻa pū kekahi RNA template reverse transcription PCR.
ʻO ke kolu, ʻaʻole hoʻokō pono ka ʻenehana DirectPCR i nā hopena PCR qualitative maʻamau ma nā ʻāpana kiko, akā loaʻa pū kekahi i ka Real-Time qPCR reactions, ka mea e koi ai i ka ʻōnaehana hopena e loaʻa i ka mana ikaika e kūʻē i ka interference fluorescence hope a e kūʻē i nā mea hoʻopau fluorescence endogenous.
ʻO ka hā, ʻo nā mea kiko kiko i manaʻo ʻia e ka ʻenehana DirectPCR e koi wale i ka hoʻokuʻu ʻana i nā mamana nucleic acid a ʻaʻole e wehe i nā proteins, polysaccharides, salt ions, etc.Pono kēia i ka nucleic acid polymerase a me ka PCR Mix i loko o ka ʻōnaehana hopena e loaʻa i ka anti-reversibility maikaʻi a me ka hoʻololi ʻana, a hiki ke hōʻoia i ka hana enzyme a me ka pololei o ka replication ma lalo o nā kūlana paʻakikī.
ʻO ka lima, ʻaʻole i hoʻokō ʻia nā ʻano kikoʻī i manaʻo ʻia e ka ʻenehana DirectPCR i kekahi lapaʻau nucleic acid enrichment, a he liʻiliʻi loa ka nui o ka template, kahi e koi ai i ka ʻike kiʻekiʻe a me ka hoʻonui ʻana o ka ʻōnaehana pane.
Ka hopena
ʻO ka ʻenehana DirectPCR kekahi o nā hoʻomohala ʻenehana koʻikoʻi a me nā mea hou i nā makahiki he 30 i hala mai ka hānau ʻana o ka ʻenehana PCR.ʻO Forgene a e hoʻomau i ka pionia a me ka mea hou o kēia ʻenehana.
He ākea loa ka manaʻolana noi o ka ʻenehana DirectPCR.ʻO ka hoʻomaikaʻi mau ʻana a me ka hoʻolaha ʻana o kēia ʻenehana e lawe mai i nā loli hoʻololi i ka noiʻi ʻepekema a me ka hana nānā.He hoʻololi ʻenehana PCR kēia.
Ka manawa hoʻouna: Feb-21-2017
