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Paʻi ʻia e: CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences

IF: 69.504

Huahana kōkua:Kelepona Huina RNA Isolation Kit

loulou kumu:https://www.nature.com/articles/s41586-022-05271-2

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ʻO nā maʻi me nā filoviruses e like me ka maʻi Ebola (EBOV) a me ka maʻi ʻo Marburg hiki ke hōʻeha i nā hōʻailona koʻikoʻi, e like me ka hemorrhagic fever a me ka hemahema multiorgan.4.Ma ʻaneʻi ua hoʻoholo mākou i ke ʻano o ka maʻi Ebola L protein i loko o ka paʻakikī me ka tetrameric VP35 me ka hoʻohana ʻana i ka microscopy cryo-electron (state 1).Ua hōʻike ʻia ka nānā ʻana i ke kūkulu ʻana i ka maʻi Ebola L he filovirus-specific insertion element i mea nui no ka RNA synthesis, a ʻo ka VP35 e launa pū me ka ʻāpana N-terminal o L e ʻekolu protomer o ka VP35 tetramer.

Ma kēia pepa, ua unuhi ʻia ka huina RNA me ForegeneKelepona Huina RNA Isolation Kita ua ana ʻia ka manaʻo o RNA e Nanodrop ma 260 nm, a laila hana i ka ʻike RT/qPCR.

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■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.

■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.

■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.

■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.

■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.

■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.

■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.


Ka manawa hoʻouna: Nov-04-2022