He mea hou paha ʻoe i ka biological, a i ʻole he luna nui me nā mākau ikaika a me ka ikaika, ua pilikia paha ʻoe i ka unuhi ʻana i ka RNA cell kiʻekiʻe?ʻEhia ka poʻe e hoʻopau i nā laʻana makamae no ka hana paʻakikī a paʻakikī o ke ʻano Trizol kuʻuna?

Ano, e hiki mai ana ka euanelio!E ʻōlelo maikaʻi loa i nā ʻano kuʻuna hoʻopau manawa a maikaʻi ʻole, a unuhi maʻalahi i ka RNA kiʻekiʻe kiʻekiʻe i 16 mau minuke.

Kelepona Huina RNA Isolation Kit

ʻAʻohe RNase o ka ʻōnaehana, ʻaʻohe pono o ka chloroform extraction, ʻaʻohe protein a me nā mea haumia ʻē aʻe, no laila hiki iā ʻoe ke hoʻomaha.

Inā he mea hou liʻiliʻi a he luna nui paha ʻoe, e loaʻa iā ʻoe kāu mau kūlana kiʻekiʻe no ka maikaʻi o ka unuhi RNA.

No laila he aha ke ʻano o ke kime kūpono?

I ka pōkole, ʻo ia ke ʻano "ʻoi aku ka maikaʻi."

ʻAʻohe pilikia, e hoʻomāʻona i kou manaʻo liʻiliʻi.

Hiki i kēia kit ke unuhi i ka RNA a pau mai nā pūnaewele i hoʻoulu ʻia ma nā papa 96, 24, 12, a me 6-well me ka puka kiʻekiʻe.Nui aʻe nā ʻano o ka RNA i hoʻihoʻi ʻia.Ma kahi o nā ʻāpana 28 a me 18 maʻamau, hiki ke hoʻohana ʻia nā 5s a me nā ʻāpana liʻiliʻi o miRNA.ʻOhi ʻia.

ʻO ke kolamu hoʻomaʻemaʻe DNA kū hoʻokahi e hiki iā ʻoe ke wehe i ka DNA genomic me ka ʻole o ka hoʻohui ʻana i ka DNase, a ʻoi aku ka maikaʻi o ka hopena, e hoʻokō i kāu mau koi hoʻokolohua koi.

I ka ʻike ʻana ma aneʻi, ua hoʻoneʻe ʻia nā mea a pau?Mai hopohopo, huahana maikaʻi, e kamaʻilio me nā ʻoiaʻiʻo!!!

一, ʻoi aku ka maʻalahi!(ʻAʻole ʻōlelo wale ʻo 16min)

二, ʻoi aku ka maikaʻi!(ʻIke ʻia ka hopena)

Foregene Cell Total RNAIsolation Kits Hoʻohālikelike i nā hopena o ke ʻano kolamu adsorption a me ke ʻano Trizol

三、Oi paʻa!(I ka wā i hoʻohana ai e ʻōlelo ʻo ia he maikaʻi)

Foregene QPCR kumu o ka gene A ma hope o ka hoʻololi ʻana

Manaʻo kōkua:

Ke haʻahaʻa loa ka nui o nā pūnaewele, e hoʻemi ʻia ka nui o ka hua RNA, a ʻaʻole hiki i ka spectrophotometer ke hoʻoholo pololei i ka waiwai OD.Manaʻo ʻia e hana i ka hoʻāʻo maikaʻi ma o nā hoʻokolohua qPCR ma hope.Hana maikaʻi kēia huahana me kā mākou hui RT EasyTM II (First-strand cDNA synthesis no Real Time PCR) (RT-01022)!

E koho i ka Foregene Cultured Cell Total RNA Extraction Kit i mea e wikiwiki ai kāu hoʻokolohua.He aha kāu e kali nei, e hana i kēia manawa!