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Hoʻolauna:

Hiki i nā molekala RNA ke launa pū me nā moleola ola ʻē aʻe, e like me ka protein, DNA a me RNA, a pēlā e noho ai i loko o nā pūnaewele a me nā mea ola ma ke ʻano o nā macromolecular complexes.I waena o lākou, ʻo RNA-protein complexes ke ola a me ka hana o RNA ʻO kekahi o nā ʻano nui o.ʻO ka huki iho ʻana o RNA he ʻano hana maʻamau no ka nānā ʻana a me ka ʻike ʻana i nā molekole RNA, ʻoi aku ka nui o nā protein e pili ana i nā molela lncRNA, a ʻike ʻia e ka hapa nui o nā mea noiʻi a me nā puke pai palapala hoʻonaʻauao mana honua.I kēia lā e haʻi aku ʻo FORREGENE iā ʻoe i ke kumu hoʻokolohua a me ke kaʻina hana ʻenehana o RNA pull-down, malia paha hiki iā ʻoe ke hoʻohana.

1. ʻO ke kumu hoʻokolohua o RNA huki iho ʻenehana:

In vitro transcription and synthesis of biotin-labelled target lncRNA or lncRNA fragments (me ka RNA fragments i hoʻohana ʻia ma ke ʻano he mana maikaʻi a maikaʻi ʻole), a hoʻomoʻi me nā ʻāpana cell;e hoʻohana i nā peʻa magnetic avidin-anchored e hōʻiliʻili i ka RNA-protein a i ʻole RNA-RNA complexes, Ma hope o ka holoi ʻana, hoʻopau ʻia ka paʻakikī.Inā ʻike ʻoe i ka molekele pahuhopu e pili ana me ka lncRNA, e hana i ka electrophoresis protein ma ka huahana RNA-huki i lalo, e ʻike i ka protein i hoʻopaʻa ʻia ma ke kala kala, a ʻoki i ka gel no ka spectrometry lehulehu;e hana pololei i ka hōʻoia komohana blot ma ka huahana huki;inā ʻike ʻia No nā molekele i hoʻopaʻa ʻia e RNA e launa pū me ka lncRNA, hoʻokomo ʻia nā huahana RNA-huki i lalo i ka unuhi RNA a ua hana ʻia ka ʻike qRT-PCR kikoʻī.

2. Huki iho ka RNA i ke kaʻina hoʻokolohua ʻenehana:

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3. ʻO ka lawelawe ʻenehana no ka huki ʻana o RNA i lalo o FOREGENE:

Moʻomeheu kelepona

Ka hoʻomākaukau ʻana i ka huina cell/nuclear/plasma protein extract

RNA huki iho hoʻokolohua

SDS-PAGE electrophoresis, kala kala

Nā hoʻokolohua mass spectrometry a me WB

Hōʻike hoʻokolohua

4. Ka hihia hoʻokolohua

Hōʻike ke kiʻi ma lalo nei i nā hopena o ka huki ʻana o ka RNA a FJ Bio-Bio a me ka ʻike electrophoresis SDS-PAGE, kahi e hōʻailona ai ka pua ʻulaʻula i ka hui ʻokoʻa.

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Ua hōʻiliʻili ka hui noiʻi o Professor Song Xu o ke Kulanui ʻo Sichuan i 15 mau makahiki o ka hōʻiliʻili lōʻihi ma ke kahua o ka noiʻi lncRNA.Ua hui pū ka hui noiʻi ʻo Foregene Bio a me Professor Song Xu e hoʻolauna i ka ʻenehana huki iho RNA o lncRNA, e hāʻawi iā ʻoe i kahi hopena maikaʻi no kāu hoʻokolohua.


Ka manawa hoʻouna: Jun-11-2021