Mai kona hoʻokumu ʻana i ka makahiki 2011, ua kālele ʻo Foregene i ka hoʻomohala ʻana i nā ʻenehana pili i ka molecular a me ka cell biology, nā huahana a me nā lawelawe.

Ma ke kamaʻilio ʻana no Foregene, pehea kou manaʻo:

ʻO ka pahu hoʻomaʻemaʻe RNA me 11 mau minuke unuhi?

ʻO ka pahu PCR pololei me ka hoʻomaʻemaʻe ʻole o ka waika nucleic?

A i ʻole he kime Cell Direct RT-qPCR e koi wale ana i 2 mau ʻanuʻu a me 7 mau minuke e kiʻi i ka template RNA?

Ua hōʻiliʻili kēlā me kēia huahana o Foregene i ka hana paʻakikī a nā poʻe āpau o ka hui mai R&D a i ka mākeke.No laila, ke lohe mākou i nā manaʻo maikaʻi mai nā mea kūʻai mai, piha mākou i ka hauʻoli.I kēlā me kēia manawa e hālāwai ai ka mea kūʻai aku i nā pilikia, e hana mākou i ka mea maikaʻi loa e nānā a loaʻa ke kumu.

Hoʻopili mau mākou i ka manaʻo o ka huahana mua a me ka lawelawe ma mua, e kālele ana i ka ʻepekema ola, a hoʻoikaika e hoʻomaʻamaʻa maʻalahi.I kēia mau lā, ua ʻike ʻia a kākoʻo ʻia nā huahana a Foregene e nā mea noiʻi ʻepekema hou aʻe.E like me ka post, ʻo ka huina o nā huaʻōlelo SCI o nā huahana Foregene he 231, ʻo ka huina hopena he 786.202, ʻo ka mea hopena kiʻekiʻe loa ʻo 17.848, a ʻo ka awelika hopena hopena he 3.403.

Hoʻopili ʻo Foregene i ka manaʻo o ka hoʻokolohua ʻoi aku ka maʻalahi, e paipai i nā ʻepekema noiʻi noiʻi e hana i ka noiʻi ʻepekema, a me ka hāʻawi ʻana i ke kōkua noiʻi ʻepekema i nā loea noiʻi ʻepekema koʻikoʻi.

Eia kekahi mau ʻatikala i paʻi ʻia a me ka ʻike huahana pili.

Holoholona Huina RNA Isolation KIT

Nā hiʻohiʻona:

ʻAʻole pono e hopohopo e pili ana i ka degradation RNA.ʻO ka ʻōnaehana holoʻokoʻa he RNase-Free.Hoʻopau maikaʻi i ka DNA me ka hoʻohana ʻana i ka DNA-Cleaning Column, wehe i ka DNA me ka ʻole o ka hoʻohui ʻana i ka DNase, maʻalahi—ua pau nā hana āpau ma ka lumi wela, wikiwiki—hiki ke hoʻopau ʻia ka hana i loko o 30 mau minuke, palekana-ʻaʻole hoʻohana ʻia ka mea hoʻonaninani, a me ka maʻemaʻe kiʻekiʻe-OD260/280≈1.8-2.1

Mea kanu Huina RNA Isolation KIT

Nā hiʻohiʻona:

ʻAʻohe DNase i koi ʻia, hiki ke wehe i ka gDNA me ka ʻole o DNase

ʻAʻole pono e hopohopo e pili ana i ka hōʻino ʻana o RNA, ʻo RNase-Free ka ʻōnaehana holoʻokoʻa, a hoʻopau ʻia ka hana ma 30Min