ALOHA, e na hoa!
Ma hope o ka hana ʻana i ka hōʻiliʻili nucleic acid no nā makahiki he nui, ʻike maikaʻi nā mea a pau i ka maikaʻi o ka ʻokiʻoki nucleic acid ke kī i ka holomua a me ka pololei o nā hoʻokolohua ma hope.
I nā manawa he nui, mālama wale mākou inā ʻo ka ratio o OD260/OD280 i loko o ka pae maikaʻi loa.E like me kēia…
E like me ka ʻōlelo ʻana, ʻaʻole hiki iā ʻoe ke ʻōlelo nui, a ʻaʻole hiki iā ʻoe ke hauʻoli i ke kakahiaka nui.
E hoʻolohe mua i ka Lunahooponopono e haʻi iā ʻoe i kahi moʻolelo liʻiliʻi!
ʻO ia mau moʻolelo moʻolelo nani e hana pinepine ʻia i loko o nā hale hana e hana ana i ka ʻokiʻoki nucleic acid.
Ua manaʻo ʻoe ua lawe wale ʻoe iā RNA, E kala mai!
ʻOiaʻiʻo, ʻaʻole ʻoe i ʻike e hūnā malū ʻo Mr Protein.
Manaʻo ʻoe makemake ʻoe e hōʻike iā Mr Protein me hoʻokahi OD260/OD280?
ʻOpiopio loa!Maʻalahi loa!
E hoʻohana mākou i ke ʻano hoʻopaʻa kālā koʻikoʻi loa e kōkua iā ʻoe e ʻike i nā protein huna i loko o RNA!
| Huahana i hoʻohana ʻia | OD260/280 | Mmea hoʻoheheʻe |
| Foregene | 2.09 | Thermo ScientificTMNanoDropTMʻekahi Hōʻike ʻike kiʻekiʻe UV-Vis Spectrophotometer |
| Trizol | 1.91 |
He mau hoaaloha pili loa ka protein a me RNA.
ʻOi aku ka hilahila o Mr Protein.
No laila ʻo ia ka RNA ikaika a hauʻoli e pāʻani me ia.
I kekahi lā.
Ua haʻi aku ʻo RNA iā Mr Protein e lawe ʻia e nā poʻe ʻē aʻe, a ua kaumaha loa ʻo Mr Protein.
ʻAʻole makemake nui ʻo RNA iā Mr protein, a ʻōlelo ʻo ia: "Pehea wau e haʻalele ai iā ʻoe, peʻe ʻoe i loko o koʻu ʻaʻahu, e hoʻāʻo e ʻike ʻole ʻia e nā poʻe ʻē aʻe, ʻae?"
Ua ʻakaʻaka ʻo Mr. Protein a ʻae ʻo ia me ka hauʻoli.
I ka la o ka hookamaia, ua huna maoli o Mr Protein i na lole o RNA a aole i loaa.Ua hahai ʻo ia i ke kanaka hānai i ka home a noho hauʻoli mai ia manawa mai.
ʻO ka hoʻohālikelike o ka unuhi ʻana o RNA me ForegeneKeleponaHuinaRNA Extraction Kit
a me T Company's Trizol Reagent
Hiki iā mākou ke ʻike maopopo i ka paʻi ʻana ma ke ala 4 (Trizol), aʻo ke ala 3 (Foregene) maʻemaʻe.Ua hōʻoia ʻia nā hopena he mau hāʻule nui nā RNA protein i lawe ʻia e ke ʻano Trizol!A ʻaneʻane ʻaʻohe haumia protein no ka mea hoʻokolohua i ka pahu hoʻoheheʻe Foregene RNA!(Ua hiki ʻole mai ka hoʻolaha)
ʻIke hou aku:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Ka manawa hoʻouna: Feb-10-2022
