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ʻElua ʻano nui o nā pilikia i loko o nā keʻena PCR: nā pilikia biosafety a me nā pōʻino o ka nucleic acid.Hoʻopilikia ka mea mua i ka poʻe a me ke kaiapuni, a pili ka hope i nā hopena o nā hoʻokolohua PCR.ʻO kēia ʻatikala e pili ana i nā wahi mākaʻikaʻi koʻikoʻi o ka PCR a me nā pae pilikia i lawe ʻia iā ʻoe.

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01 Mahele o ka hale hana PCR

1. Keʻena hoʻokolohua molecular biology

Wahi a nā koi o ka Paukū 1.1 o ka Basic Setting Standards for Clinical Gene Amplification Testing Laboratories, ʻehā mau wahi o nā laboratories PCR: kahi mālama reagent a me kahi hoʻomākaukau, wahi hoʻomākaukau specimen, wahi hoʻonui, a me kahi wahi hōʻike huahana amplification.Inā hoʻohana ʻia ke ʻano PCR fluorescent manawa maoli, hiki ke hoʻohui ʻia ka wahi hoʻonui a me ka ʻāpana loiloi i hoʻokahi wahi;inā hoʻohana ʻia ka PCR analyser automated piha, hiki ke hoʻohui ʻia ka wahi hoʻomākaukau specimen, ka wahi hoʻonui a me ka ʻāpana loiloi i hoʻokahi wahi.

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ʻO ka "Workbook for New Coronavirus Nucleic Acid Testing in Medical Institutions (Trial Version 2)" e kuhikuhi ana i ka manaʻo, pono e hoʻonohonoho nā laboratories e hana ana i ka hoʻāʻo hou coronavirus nucleic acid i kēia mau wahi: reagent storage and preparation area, specimen preparation area, amplification and product analysis area.ʻO kēia mau wahi ʻekolu e kūʻokoʻa loa i kekahi i kekahi ma kahi ākea, a ʻaʻole hiki ke kamaʻilio pololei me ka ea.

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2. lumi hoʻomākaukau laʻana

ʻOiai hiki ke hoʻomākaukau wale ʻia nā laʻana ma ka wahi hoʻomākaukau specimen, pono nō kahi lumi hoʻomākaukau kūikawā kūikawā i ka wā e pili ana i nā laʻana paʻakikī a me ka nui o nā laʻana.ʻO ka lumi hoʻomākaukau laʻana he pilikia nui o ka palekana olaola a me ka hoʻohaumia nucleic acid.

3. lumi ho'oma'ema'e 'ōpala

ʻO ka mālama ʻana i nā ʻōpala kūpono ʻole e lawe mai i nā pōʻino nui o ka biosafety a me ka ʻakika nucleic contamination i ka hale hana.No laila, pono e nānā mau ʻia ke keʻena mālama ʻōpala.

02 Nā wahi mākaʻikaʻi pilikia ma nā hale hana PCR

Hoʻokaʻawale ʻia nā keʻena hana hoʻokaʻawale i ke keʻena hoʻomākaukau laʻana, kahi mālama reagent a me kahi hoʻomākaukau, kahi hoʻomākaukau specimen, amplification a me ka nānā ʻana o nā huahana, a me ka lumi mālama ʻōpala.

E like me ke ʻano o ka sampling site, ua hoʻokaʻawale ʻia i ka ʻili, ka mea kani, ka laʻana, ka nānā ʻana i ke kaiapuni a me ka pipette.

Mai ka haʻahaʻa a i ke kiʻekiʻe mai ka hōkū hoʻokahi★ a i ʻekolu hoku★★★.

1. lumi hoʻomākaukau laʻana:

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Hoʻohana ʻia ia no ka hoʻopaʻa inoa ʻana, ka hoʻomākaukau ʻana a me ka inactivation o nā laʻana, a ʻo ka pilikia palekana olaola ka mea kiʻekiʻe loa.No ka mea, ʻaʻole i unuhi ʻia a hoʻonui ʻia nā laʻana, koe wale nā ​​pipettes i hui pinepine ʻia me nā laʻana, ʻoi aku ka liʻiliʻi o ka hoʻohaumia ʻana o ka waikawa nucleic i nā ʻāpana ʻē aʻe.

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1-4 Ka laʻana ma nā wahi nānā

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5-8 Ka laʻana ma kahi mākaʻikaʻi

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9-12 mākaʻikaʻi wahi laʻana

1. Wahi hoʻopaʻa ʻana a me ka hoʻomākaukau ʻana:

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Hoʻohana ʻia ia no ka hoʻomākaukau ʻana i nā reagents mālama, ka hāʻawi ʻana i nā reagents a me ka hoʻomākaukau ʻana i ka hui ʻana o ka amplification reaction, a me ka mālama ʻana a me ka hoʻomākaukau ʻana i nā mea hoʻohana e like me ka centrifuge tubes a me nā ʻōlelo aʻoaʻo pipette.ʻAʻohe pilina pili pono me nā laʻana a ʻaʻohe ʻakika nucleic maikaʻi ma kēia wahi, no laila he haʻahaʻa ka pilikia biosafety a me ka waika nucleic contamination.

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13-16 Ka laʻana ma nā wahi nānā

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17-22 Ka laʻana ma ka nānā ʻana
3. Nā wahi hoʻomākaukau specimen

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Hoʻohana ʻia ia e wehe i ka barela hoʻoili, hoʻopau i ka specimen (inā pili), e unuhi i ka waikawa nucleic a hoʻohui i ka paipu hopena amplification, a me nā mea ʻē aʻe.

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29 Ka laʻana ma nā wahi nānā

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4. Hoʻonui a me ka nānā ʻana i nā huahana:

Hoʻohana ʻia no ka hoʻonui ʻana i ka waikawa nucleic.ʻAʻole pili kēia ʻāpana i ka hana laʻana, a haʻahaʻa ka pilikia palekana olaola.ʻO ka nui o ka hoʻonui ʻana i ka waika nucleic ma kēia ʻāpana, a ʻo ka pilikia o ka hoʻohaumia ʻana i ka waika nucleic ka mea kiʻekiʻe loa.

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38 Ka laʻana ma nā wahi nānā
5. lumi hoʻomaʻemaʻe ʻōpala:

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Hoʻohana ʻia no ka hana kiʻekiʻe o nā laʻana.ʻO nā pilikia palekana olaola e pili ana i ka hana ʻana i nā laʻana ma kēia wahi he kiʻekiʻe.Manaʻo ʻia nā huahana amplification nucleic acid e mālama ʻia ma ke ʻano he lāʻau lapaʻau.ʻAʻole ʻōlelo ʻia ke kaomi kiʻekiʻe, a he haʻahaʻa ka pilikia o ka hoʻohaumia ʻana o ka waikawa nucleic.

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43-44 Ka laʻana ma nā wahi nānā

03 Hoʻokō

I kēia manawa ua helu mākou i 44 mau wahi nānā.Ua manaʻo ʻia he nui ka poʻe e nīnau, pono anei lākou e hana i nā helu he nui?ʻAe, hana i nā mea a pau!Manaʻo wau e hana mua ʻoe i kahi loiloi pilikia o kāu keʻena ponoʻī, hiki ke hana ʻia e like me ka pilikia mai ke kiʻekiʻe a hiki i ka haʻahaʻa, hiki iā ʻoe ke nānā pū i ke ʻano like o nā laʻana, a i ʻole hiki iā ʻoe ke kūkulu i kahi hoʻolālā laʻana no ka nānā mau.I ka pōkole, hiki i kēlā me kēia hale hana ke hana i kāna hoʻolālā hoʻokō ponoʻī e pili ana i kona kūlana ponoʻī.ʻO ka pilikia nui loa no ka hoʻāʻo ʻana i nā laboratories ʻo ka haʻalele ʻana i nā pilikia.


Ka manawa hoʻouna: Jul-03-2021