ʻElua ʻano nui o nā pilikia i loko o nā keʻena PCR: nā pilikia biosafety a me nā pōʻino o ka nucleic acid.Hoʻopilikia ka mea mua i ka poʻe a me ke kaiapuni, a pili ka hope i nā hopena o nā hoʻokolohua PCR.ʻO kēia ʻatikala e pili ana i nā wahi mākaʻikaʻi koʻikoʻi o ka PCR a me nā pae pilikia i lawe ʻia iā ʻoe.
01 Mahele o ka hale hana PCR
1. Keʻena hoʻokolohua molecular biology
Wahi a nā koi o ka Paukū 1.1 o ka Basic Setting Standards for Clinical Gene Amplification Testing Laboratories, ʻehā mau wahi o nā laboratories PCR: kahi mālama reagent a me kahi hoʻomākaukau, wahi hoʻomākaukau specimen, wahi hoʻonui, a me kahi wahi hōʻike huahana amplification.Inā hoʻohana ʻia ke ʻano PCR fluorescent manawa maoli, hiki ke hoʻohui ʻia ka wahi hoʻonui a me ka ʻāpana loiloi i hoʻokahi wahi;inā hoʻohana ʻia ka PCR analyser automated piha, hiki ke hoʻohui ʻia ka wahi hoʻomākaukau specimen, ka wahi hoʻonui a me ka ʻāpana loiloi i hoʻokahi wahi.
ʻO ka "Workbook for New Coronavirus Nucleic Acid Testing in Medical Institutions (Trial Version 2)" e kuhikuhi ana i ka manaʻo, pono e hoʻonohonoho nā laboratories e hana ana i ka hoʻāʻo hou coronavirus nucleic acid i kēia mau wahi: reagent storage and preparation area, specimen preparation area, amplification and product analysis area.ʻO kēia mau wahi ʻekolu e kūʻokoʻa loa i kekahi i kekahi ma kahi ākea, a ʻaʻole hiki ke kamaʻilio pololei me ka ea.
2. lumi hoʻomākaukau laʻana
ʻOiai hiki ke hoʻomākaukau wale ʻia nā laʻana ma ka wahi hoʻomākaukau specimen, pono nō kahi lumi hoʻomākaukau kūikawā kūikawā i ka wā e pili ana i nā laʻana paʻakikī a me ka nui o nā laʻana.ʻO ka lumi hoʻomākaukau laʻana he pilikia nui o ka palekana olaola a me ka hoʻohaumia nucleic acid.
3. lumi ho'oma'ema'e 'ōpala
ʻO ka mālama ʻana i nā ʻōpala kūpono ʻole e lawe mai i nā pōʻino nui o ka biosafety a me ka ʻakika nucleic contamination i ka hale hana.No laila, pono e nānā mau ʻia ke keʻena mālama ʻōpala.
02 Nā wahi mākaʻikaʻi pilikia ma nā hale hana PCR
Hoʻokaʻawale ʻia nā keʻena hana hoʻokaʻawale i ke keʻena hoʻomākaukau laʻana, kahi mālama reagent a me kahi hoʻomākaukau, kahi hoʻomākaukau specimen, amplification a me ka nānā ʻana o nā huahana, a me ka lumi mālama ʻōpala.
E like me ke ʻano o ka sampling site, ua hoʻokaʻawale ʻia i ka ʻili, ka mea kani, ka laʻana, ka nānā ʻana i ke kaiapuni a me ka pipette.
Mai ka haʻahaʻa a i ke kiʻekiʻe mai ka hōkū hoʻokahi★ a i ʻekolu hoku★★★.
1. lumi hoʻomākaukau laʻana:
Hoʻohana ʻia ia no ka hoʻopaʻa inoa ʻana, ka hoʻomākaukau ʻana a me ka inactivation o nā laʻana, a ʻo ka pilikia palekana olaola ka mea kiʻekiʻe loa.No ka mea, ʻaʻole i unuhi ʻia a hoʻonui ʻia nā laʻana, koe wale nā pipettes i hui pinepine ʻia me nā laʻana, ʻoi aku ka liʻiliʻi o ka hoʻohaumia ʻana o ka waikawa nucleic i nā ʻāpana ʻē aʻe.
1-4 Ka laʻana ma nā wahi nānā
5-8 Ka laʻana ma kahi mākaʻikaʻi
9-12 mākaʻikaʻi wahi laʻana
1. Wahi hoʻopaʻa ʻana a me ka hoʻomākaukau ʻana:
Hoʻohana ʻia ia no ka hoʻomākaukau ʻana i nā reagents mālama, ka hāʻawi ʻana i nā reagents a me ka hoʻomākaukau ʻana i ka hui ʻana o ka amplification reaction, a me ka mālama ʻana a me ka hoʻomākaukau ʻana i nā mea hoʻohana e like me ka centrifuge tubes a me nā ʻōlelo aʻoaʻo pipette.ʻAʻohe pilina pili pono me nā laʻana a ʻaʻohe ʻakika nucleic maikaʻi ma kēia wahi, no laila he haʻahaʻa ka pilikia biosafety a me ka waika nucleic contamination.
13-16 Ka laʻana ma nā wahi nānā
17-22 Ka laʻana ma ka nānā ʻana
3. Nā wahi hoʻomākaukau specimen
Hoʻohana ʻia ia e wehe i ka barela hoʻoili, hoʻopau i ka specimen (inā pili), e unuhi i ka waikawa nucleic a hoʻohui i ka paipu hopena amplification, a me nā mea ʻē aʻe.
29 Ka laʻana ma nā wahi nānā
4. Hoʻonui a me ka nānā ʻana i nā huahana:
Hoʻohana ʻia no ka hoʻonui ʻana i ka waikawa nucleic.ʻAʻole pili kēia ʻāpana i ka hana laʻana, a haʻahaʻa ka pilikia palekana olaola.ʻO ka nui o ka hoʻonui ʻana i ka waika nucleic ma kēia ʻāpana, a ʻo ka pilikia o ka hoʻohaumia ʻana i ka waika nucleic ka mea kiʻekiʻe loa.
38 Ka laʻana ma nā wahi nānā
5. lumi hoʻomaʻemaʻe ʻōpala:
Hoʻohana ʻia no ka hana kiʻekiʻe o nā laʻana.ʻO nā pilikia palekana olaola e pili ana i ka hana ʻana i nā laʻana ma kēia wahi he kiʻekiʻe.Manaʻo ʻia nā huahana amplification nucleic acid e mālama ʻia ma ke ʻano he lāʻau lapaʻau.ʻAʻole ʻōlelo ʻia ke kaomi kiʻekiʻe, a he haʻahaʻa ka pilikia o ka hoʻohaumia ʻana o ka waikawa nucleic.
43-44 Ka laʻana ma nā wahi nānā
03 Hoʻokō
I kēia manawa ua helu mākou i 44 mau wahi nānā.Ua manaʻo ʻia he nui ka poʻe e nīnau, pono anei lākou e hana i nā helu he nui?ʻAe, hana i nā mea a pau!Manaʻo wau e hana mua ʻoe i kahi loiloi pilikia o kāu keʻena ponoʻī, hiki ke hana ʻia e like me ka pilikia mai ke kiʻekiʻe a hiki i ka haʻahaʻa, hiki iā ʻoe ke nānā pū i ke ʻano like o nā laʻana, a i ʻole hiki iā ʻoe ke kūkulu i kahi hoʻolālā laʻana no ka nānā mau.I ka pōkole, hiki i kēlā me kēia hale hana ke hana i kāna hoʻolālā hoʻokō ponoʻī e pili ana i kona kūlana ponoʻī.ʻO ka pilikia nui loa no ka hoʻāʻo ʻana i nā laboratories ʻo ka haʻalele ʻana i nā pilikia.
Ka manawa hoʻouna: Jul-03-2021