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Kiko PCR Pono Mea kanu I/II (me ka ʻole o nā mea hana laʻana) Kūkākūkā Mea kanu pono PCR Kit

Kiko PCR Pono Meakanu I/II (me ka ʻole o nā mea hana laʻana) Kūkākūkā Mea kanu PCR Kits Kiʻi Hōʻike ʻia.
  • Kiko PCR Pono Mea kanu I/II (me ka ʻole o nā mea hana laʻana) Kūkākūkā Mea kanu pono PCR Kit
  • Kiko PCR Pono Mea kanu I/II (me ka ʻole o nā mea hana laʻana) Kūkākūkā Mea kanu pono PCR Kit
  • Kiko PCR Pono Mea kanu I/II (me ka ʻole o nā mea hana laʻana) Kūkākūkā Mea kanu pono PCR Kit
  • Kiko PCR Pono Mea kanu I/II (me ka ʻole o nā mea hana laʻana) Kūkākūkā Mea kanu pono PCR Kit

ʻO ka wehewehe ʻana o ka pahu:

◮ʻAʻohe hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi (Hoʻopakele ka manawa, hana a me ke kumukūʻai!)

◮Liʻiliʻi mea

◮Maʻalahi—hiki ke hāʻawi ʻia ka laʻana i ka hopena PCR a i ʻole ka hopena lysis me ka ʻoki ʻole a i ʻole ka wili ʻana

◮2 X Mix—e hoemi ana i na hewa hoouka ana a me ka manawa hoomakaukau nenoaiu

◮Hiki ke hoʻomākaukau wikiwiki i ka 10 minuke, hiki ke hoʻopau i ka hopena PCR i 50 mau minuke

◮Hiki ke hoʻopau ʻia ka hopena kiʻekiʻe-lysis ma 96-well PCR plate

ikaika mua


Hoʻoiho ma ke ʻano he PDF

Huahana Huahana

Huahana Huahana

Nā wehewehe

Hoʻohana kēia huahana i kahi ʻōnaehana lysis buffer kūʻokoʻa e lyse i nā hua kanu.Hiki ke hoʻohana ʻia ka lysate ma ke ʻano he laʻana me ka hoʻomaʻemaʻe ʻole.Ma hope o ka hoʻohui ʻana i nā primers, hiki ke hoʻohana ʻia ka PCR Mix o kēia kit no ka hoʻonui.

Nā kikoʻī

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nā ʻāpana pahu

Puke PCR pololei i na hua kanu I

Mahele I

Hoʻopaʻa SP1

Hoʻopaʻa SP2

6×DNA Loading Buffer

Mahele II

2×Seed PCR maʻalahiTMHuikau

Mea kanu Meakano Direct PCR Kit II

Mahele I

Hoʻopaʻa SP1

Hoʻopaʻa SP2

6×DNA Loading Buffer

Mahele II

2×Seed PCR maʻalahiTMHuikau

Nā hiʻohiʻona & pono

■ ʻAʻohe hoʻomaʻemaʻe DNA hoʻopau manawa a pipiʻi

■ He mea uuku

■ Maalahi—hiki ke hāʻawi ʻia ka hāpana i ka PCR reaction a i ʻole lysis reaction me ka ʻoki ʻole ʻana a i ʻole ka wili ʻana

■ 2X Hui—e ho'ēmi ana i nā hewa hoʻouka hāpana a me ka manawa hoʻomākaukau ʻōnaehana pane

■ wikiwiki—Hiki ke hoʻopau ʻia ka hoʻomākaukau ʻana ma 10 mau minuke, hiki ke hoʻopau ʻia ka hopena PCR i 50 mau minuke

■ Kiʻekiʻe o ka hana—Hiki ke hoʻopau ʻia ka hopena lysis ma ka pā PCR 96-well.

Nā palena kime

Noi: Hoʻomaopopo i nā mea kanu transgenic, genotyping, etc.

Laʻana: Kanu i nā ʻanoʻano a i ʻole nā ​​ʻiʻo me ka polysaccharide haʻahaʻa a me ka polyphenol content.

Laʻana nui: Hoʻokahi hua holoʻokoʻa, 1-100mg mea kanu

Laulā ʻike: huahana PCR ≤1kb

Ka holo hana

kanu hua kanu PCR

Electtropherogram

mea kanu pololei PCR-DIAGRAM
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