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ʻO ka mea kanu Hui Pū ʻIa RNA Isolation Kit Huina RNA Purificaiton Kit no nā mea kanu haʻahaʻa i loko o nā Polysaccharides a me nā Polyphenols

ʻO ka wehewehe ʻana o ka pahu:

Cat.No.RE-05011/05014

No ka hoʻomaʻemaʻe ʻana i ka RNA āpau mai nā laʻana mea kanu maʻamau i loaʻa nā polysaccharide haʻahaʻa a me nā ʻāpana polyphenol.

Wehe koke i ka RNA holoʻokoʻa kiʻekiʻe mai nā mea kanu me ka polysaccharide haʻahaʻa a me ka polyphenol content.

RNase-Free

Hoʻopau maikaʻi i ka DNA me ka hoʻohana ʻana i ke kolamu hoʻomaʻemaʻe DNA

Wehe i ka DNA me ka hoʻohui ʻole i ka DNase

Maʻalahi—ua pau nā hana a pau i ka wela lumi

Hiki ke hoʻopau i ka hana i loko o 30 mau minuke

Palekana—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik


Huahana Huahana

Huahana Huahana

FAQ

HOOLAHA

Nā kikoʻī

50 Mākaukau, 200 Mākaukau

Hoʻohana ka kit i ke kolamu spin a me ka formula i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA holoʻokoʻa kiʻekiʻe a kiʻekiʻe mai nā ʻiʻo mea kanu me nā polysaccharides haʻahaʻa a me nā polyphenols.No nā laʻana mea kanu me nā polysaccharides kiʻekiʻe a i ʻole polyphenols, pono e hoʻohana i ka Plant Total RNA Isolation Plus Kit no ka loaʻa ʻana o nā hopena unuhi RNA maikaʻi aʻe.Hāʻawi ka pahu i ke kolamu hoʻomaʻemaʻe DNA e hiki ke wehe maʻalahi i ka DNA genomic mai ka supernatant a me ka lysate kiko.Hiki i ke kolamu RNA-wale ke hoopaa pono i ka RNA.Hiki i ke kit ke hana i ka nui o nā laʻana i ka manawa like.

ʻAʻole i loko o ka ʻōnaehana holoʻokoʻa RNase, no laila ʻaʻole e hoʻohaʻahaʻa ʻia ka RNA i hoʻomaʻemaʻe ʻia.Hiki i ka Buffer PRW1 a me ka Buffer PRW2 ke hōʻoia ʻaʻole i haumia ka RNA i loaʻa e ka protein, DNA, nā ion, a me nā pūhui organik.

Nā ʻāpana pahu

Hoʻopaʻa PSL1, Hoʻopaʻa PS, Hoʻopaʻa PSL2

Hoʻopaʻa PRW1, Hoʻopaʻa PRW2

RNase-Free ddH2ʻO, DNA-Cleaning Column

RNA-Kola wale nō

Nā kuhikuhi

Nā hiʻohiʻona & pono

■ Ka hana ma ka lumi wela (15-25 ℃) ma ke kaʻina holoʻokoʻa, me ka ʻauʻau hau a me ka centrifugation haʻahaʻa wela.
■ Hoʻopiha piha RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopili pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki ai i ka pahu ke hoʻoneʻe i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi e hana a hiki ke hoʻopau i loko o 30 mau minuke.
■ Palekana: ʻaʻole koi ʻia ka reagent organik.
■ Kiʻekiʻe: ʻO nā ʻāpana RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā noi hoʻokolohua like ʻole.

123

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA a pau mai nā laʻana o nā mea kanu hou a i ʻole ka hau (ʻoi aku ka ʻiʻo o ka lau mea kanu hou) me ka polysaccharide haʻahaʻa a me ka polyphenol content.

Ka holo hana

kanu i ka RNA holoʻokoʻa maʻalahi

Diagram

Mea kanu Huina RNA Isolation Kit6

ʻO ka Plant Total RNA Isolation Kit Plus i hana ʻia he 50mg o nā lau hou o polysaccharides a me polyphenols, a ua hoʻāʻo ʻia ʻo 5% RNA i hoʻomaʻemaʻe ʻia e ka electrophoresis.
1: Maiʻa
2: Ginkgo
3: pulupulu
4: Pomegerane

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mau mahina ma ka lumi wela (15–25 ℃) ma kahi maloʻo, a me 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ka Buffer PSL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho).


  • Mua:
  • Aʻe:

  • Ke alakaʻi hoʻoponopono pilikia

    ʻO kēia ka nānā ʻana o nā pilikia āu e hālāwai ai maHuina MeakanuRNA extraction will help you with your experiments. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali : Tech@foregene.com.

    Paʻa ke kolamu wili

    ʻO ka hoʻopaʻa ʻia ʻana o ke kolamu milo e hoʻemi ʻia ka hua RNA a i ʻole hiki ke hoʻomaʻemaʻe ʻia no ka loaʻa ʻana o RNA, a e haʻahaʻa ka maikaʻi o ka RNA i loaʻa.

    Ka Ikepili Kumu ma'amau:

    1. ʻAʻole nahā loa ka hāpana.

    Hiki i ka ʻāpana hāpana piha ʻole ke ālai i ke kolamu hoʻomaʻemaʻe DNA, hiki ke hoʻopilikia i ka hua RNA a me ka maikaʻi.Manaʻo mākou i ka wā e hana ai i ka hoʻokaʻawale ʻana i ka laʻana, e wili koke i ka nui o ka naikokene wai e wāwahi i nā ʻiʻo e like me nā paia cell a me nā membrane cell o nā laʻana e like me ka hiki.No nā laʻana mea kanu o polyphenol polysaccharides, paipai mākou iā ʻoe e hoʻohana i ka Plant Total RNA Isolation Kit Plus.

    2. Aspirate i ka DNA-Hoʻomaʻemaʻe Column isolated supernatant, aspirate i ka hiki cell debris pellet.

    Hiki ke hoʻopaʻa ʻia ka pellet o ka ʻōpala aspirated cell i ke kolamu RNA-wale nō i ka wā o nā kaʻina hana adsorption RNA (e nānā i ka ʻanuʻu 5 o ke kaʻina hana, ka ʻanuʻu 6 o ke kaʻina hana polysaccharide polyphenol).Manaʻo mākou e mālama pono i ka wā e hoʻomoʻi ai i kēia supernatant e pale aku i ka ʻōpala o nā cell e aspirated.

    3. He nui loa ka helu mua o ka hāpana.

    ʻO ka hoʻohana nui ʻana i ka laʻana e hopena i ka ʻāpana hāpana piha ʻole a i ʻole lysis piha ʻole o nā cell e Buffer PRL1 a i ʻole Buffer PSL1, e hopena ai i kahi kolamu hoʻomaʻemaʻe paʻa no nā hana hoʻomaʻemaʻe.Loaʻa iā Plant Total RNA Isolation Kit ka palena mua o 50 mg no ka hoʻomaʻemaʻe hoʻokahi o kahi laʻana i hana ʻia.No nā laʻana mea kanu o polyphenol polysaccharides, paipai mākou iā ʻoe e hoʻāʻo i ka Plant Total RNA Isolation Kit Plus.

    4. He haʻahaʻa loa ka mahana o ka centrifuge.

    ʻO ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe RNA holoʻokoʻa koe wale nō ka hoʻopau ʻana o ka naikokene wai o ka ʻiʻo hāpana, hana ʻia nā ʻanuʻu āpau ma ka lumi wela (20-25 °C).ʻO kekahi mau centrifuges haʻahaʻa haʻahaʻa he mau wela ma lalo o 20 °C, hiki ke hoʻopaʻa i ke kolamu hoʻomaʻemaʻe DNA a/a i ʻole RNA wale nō.Inā loaʻa kēia, hoʻonoho i ka mahana centrifuge i 20-25 °C a hoʻomaʻamaʻa mua i ka hui lysis a/a i ʻole ka hoʻohui ʻana o ka supernatant hoʻokaʻawale ethanol i 37 °C.

    ʻAʻohe RNA i unuhi ʻia a haʻahaʻa paha ka hua RNA

    Nui nā kumu e pili ana i ka pono o ka hoʻihoʻi ʻana, e like me: sample RNA content, operation method, the elution volume, etc.

    Ka nānā 'ana i nā kumu ma'amau e like me lalo:

    1. Ua hana ʻia kahi ʻauʻau hau a i ʻole ka wela haʻahaʻa (4 ° C) centrifugation i ka wā o ka hana.

    Manaʻo: E hana ma ka lumi wela (15-25 ° C) i ke kaʻina holoʻokoʻa, mai hana i ka hau hau a me ka centrifugation haʻahaʻa haʻahaʻa.

           2.Ua hoʻohaʻahaʻa ʻia ka RNA ma muli o ka mālama pono ʻole ʻia o ka laʻana a i ʻole ka mālama ʻana i ka laʻana no ka wā lōʻihi.

    Manaʻo Manaʻo: Pono e hoʻopaʻa koke ʻia nā laʻana i hōʻiliʻili hou ʻia i loko o ka hau hau, a laila mālama ʻia ma -80 ° C no ka manawa lōʻihi, e pale i ka hoʻoheheʻe pinepine ʻana a me ka hoʻoheheʻe ʻana o nā laʻana;a i ʻole e hoʻoinu koke i nā laʻana i loko o ka solution RNA stabilizer RNAlater solution (nā laʻana holoholona).

           3.ʻAʻole lawa ka hāpana ʻāpana a me ka lysis alakaʻi i ka blockage o ke kolamu hoʻomaʻemaʻe.

    Manaʻo Manaʻo: I ka wā e wili ai i ka ʻiʻo, e ʻoluʻolu e hoʻopaʻa pono i ka lepo, a e hoʻoneʻe koke iā ia i ka Buffer PSL1 i hoʻomākaukau mua ʻia (e hōʻoia ua hoʻohui ʻia ka māhele kūpono o β-ME, e ʻike i ka pae 1 o ke kaʻina hana).

    4. Ua hoʻohui hewa ʻia ka eluent.

    Manaʻo: E hōʻoia i ka RNase-Free ddH2Hoʻokuʻu ʻia ʻo O i waenakonu o ka membrane kolamu hoʻomaʻemaʻe.

    5. ʻAʻole i hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer PSL2 a i ʻole Buffer PRW2.

    Manaʻo Manaʻo: E ʻoluʻolu e hahai i nā ʻōlelo kuhikuhi, e hoʻohui i ka leo kūpono o ka ethanol piha i ka Buffer PSL2 a me Buffer PRW2 a hui maikaʻi ma mua o ka hoʻohana ʻia ʻana o ka pahu.

    6. ʻAʻole kūpono ka nui o nā kiko kiko.

    Manaʻo Manaʻo: E hoʻohana i 50 mg o ka ʻiʻo no 500 μl o Buffer PSL1.ʻO ka hoʻohana ʻana i ka ʻiʻo nui e hōʻemi i ka nui o ka RNA i unuhi ʻia a e hōʻemi pū ʻia ka maʻemaʻe o ka RNA hopena.Manaʻo ikaika mākou ʻaʻole pono e ʻoi aku ka nui o ka hoʻohālike mua ma mua o 50 mg no ka hana unuhi RNA.

    7. Ka nui elution kūpono ʻole a i ʻole ka elution piha ʻole.

    Manaʻo: ʻO ka leo eluent o ke kolamu hoʻomaʻemaʻe he 50-200 μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono ia e hoʻolōʻihi i ka manawa ma ka lumi wela ma hope o ka hoʻohui ʻana i ka RNase-Free ddH preheated.2ʻO, e like me 5-10min.

    8. He koena ethanol ka kolamu hoʻomaʻemaʻe ma hope o ka holoi ʻana me Buffer PRW2.

       Manaʻo Manaʻo: Inā centrifuged ka paipu ʻole no 1 min a aia nō ka ethanol i koe ma hope o ka holoi ʻana i Buffer PRW2, hiki iā ʻoe ke hoʻonui i ka manawa o ka centrifugation paipu ʻole i 2 min, a i ʻole e kau i ke kolamu hoʻomaʻemaʻe ma ka lumi wela no 5 min e wehe loa i ke koena ethanol.

    9. Ua hoʻohana hewa ʻia ka pahu.

       Manaʻo Manaʻo: No nā laʻana mea kanu o polyphenolic polysaccharides, me ka hoʻohana ʻana i nā pahu maʻamau e like me Plant Total RNA Isolation Kit ʻaʻole hiki ke loaʻa nā laʻana RNA kūpono.Paipai mākou iā ʻoe e hoʻohana i ka Plant Total RNA IsolationKit Plus, i hoʻolālā kūikawā ʻia no nā laʻana mea kanu polyphenolic polysaccharide.He kit i hoʻomohala kūikawā no ka unuhi ʻana i ka RNA mai nā laʻana mea kanu polyphenol a me polysaccharide.

    He haʻahaʻa ka waiwai OD260/OD280

    RNA elution me ddH2ʻO a hoʻohana ʻia no ka heluhelu spectrophotometer i loaʻa nā waiwai haʻahaʻa OD260/OD280.Manaʻo mākou e hoʻohana i ka 10 mM Tris-HCl, pH 7.5 (ma mua o RNase-Free ddH2O e hoʻokaʻawale i ka RNA) no ka loaʻa ʻana o nā waiwai OD260/OD280 pololei, e nānā i ka "RNA Concentration and Purification Assays" ma ka ʻaoʻao 19.

    Hoʻohaʻahaʻa ʻia ka RNA i hoʻomaʻemaʻe ʻia

    ʻO ka maikaʻi o ka RNA i hoʻomaʻemaʻe ʻia e pili ana i nā mea e like me ka mālama ʻana i ka laʻana, ka haumia RNase, a me ka manipulation.

    Ka nānā 'ana i nā kumu ma'amau:

    1.ʻAʻole i mālamaʻia nā'āpana kiko i ka manawa ma hope o ka hōʻiliʻiliʻana.

        Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia nā laʻana kikoʻī i ka manawa ma hope o ka hōʻiliʻili ʻana, e ʻoluʻolu e mālama iā lākou i loko o ka nitrogen wai i ka haʻahaʻa haʻahaʻa koke a i ʻole e hoʻololi iā lākou i -80°C no ka mālama lōʻihi ma hope o ka hoʻomaloʻo wikiwiki ʻana i loko o ka nitrogen wai, a i ʻole e hoʻokomo koke i nā laʻana i loko o ka RNA stabilizer RNAlater solution (nā mea hoʻohālike holoholona).No ka unuhi RNA, e ho'āʻo e hoʻohana i nā laʻana kikokikona i hōʻiliʻili hou ʻia.

    2. Hoʻohou hou i ka maloʻo a me ka hoʻoheheʻe ʻana i nā ʻāpana kiko.

       Manaʻo Manaʻo: I ka mālama ʻana i nā laʻana ʻiʻo, ʻoi aku ka maikaʻi e ʻoki i nā ʻāpana liʻiliʻi no ka mālama ʻana, a lawe i kahi ʻāpana o ia mau mea i ka wā e hoʻohana ai i mea e pale ai i ka hōʻino ʻana o ka RNA ma muli o ka hoʻomaloʻo pinepine ʻana a me ka hoʻoheheʻe ʻana o nā laʻana.

    3. Hoʻokomo ʻia ʻo RNase i loko o ka lumi hana a ʻaʻole i hoʻohana ʻia i nā mīkina lima hoʻolele, masks, etc.

       Manaʻo Manaʻo: ʻoi aku ka maikaʻi o nā hoʻokolohua RNA extraction i nā hana RNA kaʻawale, a pono e hoʻomaʻemaʻe ʻia ka papa ʻaina ma mua o ka hoʻokolohua, a pono e ʻaʻahu ʻia nā mīkina lima a me nā masks i ka wā o ka hoʻokolohua e pale aku ai i ka hōʻino ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase i ka nui loa.

    4. Ua haumia ka reagent e RNase i ka wā e hoʻohana ai.

       Manaʻo Manaʻo: E hoʻololi me kahi pūʻulu hou o nā pahu hōʻiliʻili RNA piha no nā hoʻokolohua pili.

    5. ʻO nā paipu centrifuge a me nā ʻōlelo aʻoaʻo pipette i hoʻohana ʻia no ka hoʻoponopono RNA ua haumia me RNase.

    Manaʻo Manaʻo: E hōʻoia i ka RNase-Free nā paipu centrifuge, nā ʻōlelo aʻoaʻo pipette, nā pipette, a me nā mea ʻē aʻe i hoʻohana ʻia i ka unuhi RNA.

    Nā Papa kuhikuhi:

    Ka Papa Hoʻonaʻauao RNA Isolation Kit Hui Pū ʻIa

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