Mea Kūkulu Hoʻolaha RNA Huina

Mea Kūkulu Hoʻolaha RNA Huina

Pahu Wehewehe:

Hoʻohana ka pahu i ka kolamu wili a me ka haʻilula i hoʻomohala ʻia e Foregene, kahi e hiki ai ke huki pono i ka piha piha piha me ka piha kiʻekiʻe RNA mai nā ʻano mea kanu me nā polysaccharides haʻahaʻa a me nā ʻike polyphenols. No nā mea kanu me nā polysaccharides kiʻekiʻe a i ʻole ka polyphenols ʻike, noi ʻia e hoʻohana i ka Plant Total RNA Isolation Plus Kit e kiʻi i nā hopena kāʻili RNA ʻoi aku ka maikaʻi. Hāʻawi ka kit i ka kolamu DNA-hoʻomaʻemaʻe i hiki ke hemo maʻalahi i ka genomic DNA mai ka supernatant a me ka lysateʻaʻaʻa. Hiki i ke kolamu RNA ke hoʻopaʻa pono iā RNA. Hiki i ka kit ke hana i kahi nui o nā laʻana i ka manawa like.

ʻAʻohe o RNase ka ʻōnaehana holoʻokoʻa, no laila ʻaʻole e hoʻohaʻahaʻa ʻia ka RNA hoʻomaʻemaʻe. Hiki i ka Buffer PRW1 a me ka Buffer PRW2 ke hōʻoia i ka haumia ʻole o ka RNA i loaʻa ʻia e ka protein, DNA, nā ion, a me nā mea hoʻohui ʻokanika.


ʻIke kikoʻī

Nā huahana huahana

Hoakaka

50 Preps, 200 Preps

Nā ʻūmiʻi kit

Buffer PSL1, Buffer PS, Buffer PSL2

Buffer PRW1, Buffer PRW2

RNase-Free ddH2E, Kolamu Hoʻomaʻemaʻe DNA

Kolamu RNA-Wale

Nā ʻōlelo aʻo

Hiʻona & pono

■ Hana ma ke ana wela o ka lumi (15-25 ℃) ma loko o ke kaʻina holoʻokoʻa, me ka ʻole o ka ʻauʻau hau a me ka centrifugation wela haʻahaʻa.
■ Piha piha RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hoʻohaʻahaʻa RNA.
■ Hoʻopaʻa pololei ke kolamu hoʻomaʻemaʻe DNA i DNA, i hiki i ka pahu ke lawe i nā genomic DNA haumia me ka ʻole o DNase.
■ Hua kiʻekiʻe RNA: ʻO ke kolamu RNA wale nō a me kahi hana kūlike i hiki ke hoʻomaʻemaʻe maikaʻi iā RNA.
■ Wikiwiki: wikiwiki e hana a hiki ke hoʻopau ma waena o 30 mau minuke.
■ Palekana: ʻaʻole koi ʻia kahi mea ola lāʻau.
■ Kūlana kiʻekiʻe: ʻO nā ʻāpana RNA i hoʻomaʻemaʻe ʻia o ke kiʻekiʻe kiʻekiʻe, me ka ʻole o ka protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai i nā noi hoʻokolohua i lalo.

Palapala noi kini

Kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe o ka huina RNA mai nā laʻana a me nā mea kanu i hoʻoulu ʻia (ʻoi aku ka nui o nā lau lau mea kanu) me ka haʻahaʻa polysaccharide a me ka ʻike polyphenol.

Kahe ka hana

plant total RNA-simple workflow

Kiʻikuhi

Plant Total RNA Isolation Kit6

Hoʻomohala ʻia ka Plant Total RNA Isolation Kit Plus 50mg o nā lau hou o nā polysaccharides a me nā polyphenols, a ua hoʻāʻo ʻia ʻo RNA 5% e ka electrophoresis.
1: maiʻa
2: ʻO Ginkgo
3: pulupulu
4: Pomegerane

Ka mālama a me ke ola papa

Hiki ke mālama ʻia ke kini i 12 mau mahina ma ka mahana o ka lumi (15-25 ℃) ma kahi maloʻo, a me 2-8 ℃ no ka manawa lōʻihi (24 mahina).

Hiki ke mālama ʻia ʻo Buffer PSL1 ma 4 ℃ no hoʻokahi mahina ma hope o ka hoʻohui ʻana i 2-hydroxy-1-ethanethiol (pono ʻole).


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