He ʻenehana ʻo RT-PCR e hoʻohui i ka transcription huli RT o RNA a me ka polymerase chain amplification (PCR) o cDNA.Hiki ke hoʻohana ʻia ka ʻenehana RT-PCR no ka ʻike ʻana i ke kiʻekiʻe o ka hōʻike gene i loko o nā cell / tissues, ka ʻike o nā maʻi virus RNA i loko o nā cell, a me ka hoʻopili pololei ʻana i nā kaʻina cDNA o nā ʻano kikoʻī.
KA PONO NUI:
✱ He paʻa paʻa ko Foregene's RT kit ma 50-65 ℃ ka wela o ka hopena (hiki i ka 65 ° C ke wehe i ka pae ʻelua o RNA e hoʻomau i ka hoʻololi ʻana i ka hoʻololi ʻana)
✱ ʻO ka hoʻomanawanui ikaika, hiki ke ʻae i kekahi ethanol a me ka paʻakai guanidine
| sub-moʻo | inoa mea kūʻai | Nā kikoʻī | Helu Helu | Nā Kūlana Waihona |
| PCR Mix Series | 5ml | PH-01013 | -20 ℃ | |
| 40ml | PH-01015 | |||
| 5ml | PH-01013-B | -20 ℃ | ||
| 40ml | PH-01015-B | |||
| 1ml | DP-20031 | -20 ℃ | ||
| 3×1.7ml | DP-20032 | |||
| 3×1.7ml | DP-20032-B | -20 ℃ | ||
| 50ml | DP-20034-B | |||
| 2×Rapid PCR reaction premix system 2× PCR Mix | 1ml | DP-20041 | -20 ℃ | |
| 3×1.7ml | DP-20042 | |||
| 500 ×20μl rxns | QP-01012 | -20 ℃ | ||
| 2000 ×20μl rxns | QP-01014 | |||
| 500 ×20μl rxns | QP-01022 | -20 ℃ | ||
| 2000 ×20μl rxns | QP-01024 | |||
| RT Series | 25× 20μl Nā pane | RT-01011 | -20 ℃ | |
| 100 × 20μl pane | RT-01012 | |||
| (Master Premix no ke kaula cDNA synthesis no ka PCR Manawa maoli) | 200 × 10μl pane | RT-01022 | -20 ℃ | |
| 800 × 10μl pane | RT-01023 | |||
| Master Premix no ka strand cDNA synthesis mua no Real Time PCR me gDNase | 100 × 20μl pane | RT-01032 | -20 ℃ | |
| Lnc-RT HeroTM I(Me gDNase)(Super Premix no ke kaula cDNA synthesis mua mai lncRNA) | 25 × 20μl pane | RTL-09098 | -20 ℃ | |
| 50 × 20μl pane | RTL-09099 | |||
| Moʻo RT-PCR | 100 rxns | RT-02011 | -20 ℃ | |
| 500 rxns | RT-02012 | |||
| 100 rxns | RT-02021 | -20 ℃ | ||
| 500 rxns | RT-02022 | |||
| RT-qPCR Series | 100 rxns | RT-02111 | -20 ℃ | |
| 500 rxns | RT-02112 | |||
| 100 rxns | RT-02131 | -20 ℃ | ||
| 500 rxns | RT-02132 | |||
| Hoailona DNA | 50 Hoʻomākaukau | DL-20011 | 4℃ | |
| 200 Hoʻomākaukau | DL-20012 | |||
| 50 Hoʻomākaukau | DL-30011 | 4℃ | ||
| 200 Hoʻomākaukau | DL-30012 |
