RT Easyᵀᴹ I Master Premix no ka hoʻohui ʻana i ka cDNA strand mua
Nā wehewehe kime
ʻO FOREGENE's 2× RT maʻalahiTMHiki i ka hui ke hoʻohui i ka cDNA kaula mua ma kahi wela a hiki i ka 50°C, he mea kūpono ia i ka hoʻihoʻi ʻana i ka hoʻololi ʻana o nā kumu hoʻohālike RNA paʻakikī lua.
Loaʻa i ka RNA i hoʻomaʻemaʻe ʻia i loko o ke keʻena keʻena i ka waiʻona a me nā koena paʻakai guanidine, he mau mea hoʻoneʻe ikaika i ka hapa nui o nā transcriptases hoʻohuli, e hopena i nā hopena transcription huli ʻole a i ʻole ka haʻahaʻa haʻahaʻa.FOREGENE 2× RT MaʻalahiTMHōʻike ka Mix i ka hoʻomanawanui kiʻekiʻe loa i ka waiʻona a me ka paʻakai guanidine, ʻo ka hoʻomanawanui kiʻekiʻe loa i ka waiʻona i nā laʻana RNA he 45%, a ʻo ka ʻae kiʻekiʻe loa i ka paʻakai guanidine he 750mM.ʻOiai inā ʻaʻole maʻemaʻe ka RNA, hiki ke hoʻokō ʻia ka hopena transcription me ka FORREGENE 2×RT Mix.ʻO ka ʻōnaehana pane kū hoʻokahi e maʻalahi ka pane RT, wikiwiki a ʻoi aku ka maikaʻi, a hiki ke hoʻopau ʻia ka synthesis o ka strand cDNA mua i 25 mau minuke.
Hiki ke hoʻohana ʻia kēia huahana i hui pū me Foregene's fluorescent quantitative product Real Time PCR EasyTMe kiʻi i nā hopena hoʻokolohua kiʻekiʻe.
Nā kikoʻī
RT maʻalahiTMI Master Premix no ke kaula cDNA synthesis mua | ||
Kiko maʻiʻo | RT-01011 | RT-01012 |
25 manawa (20μl pūnaewele) | 100 manawa (20μl pūnaewele) | |
2× RT EasyTM Mix | 0.25ml | 1ml |
Puka Lapaʻa (50μM) | 50μl | 200μl |
Oligo(dT)18 Puna (50μM) | 50μl | 200μl |
RNase-Free ddH2O | 1.7ml | 1.7ml |
Palapala kuhikuhi | 1 apana | 1 apana |
Nā hiʻohiʻona & pono
- ʻO ka ʻōnaehana transcription maikaʻi loa, he 25 mau minuke wale nō e hoʻopau i ka synthesis o ka cDNA strand mua.
- Kiʻekiʻe-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
- He kiʻekiʻe ka wela o ka ʻōnaehana hoʻohuli, hiki ke kiʻekiʻe ke kiʻekiʻe o ka hopena o ka ʻōnaehana e like me 50 ℃, a he maikaʻi kāna hana hoʻohuli.
- ʻO nā laʻana RNA haumia (ka waiʻona a hiki i ka 45%, ka paʻakai guanidine a hiki i 750mM) hiki ke hoʻololi i ka hopena transcription.
Ke noi pahu
- RT-PCR maʻamau
- Synthesis o cDNA no ka noiʻi cloning a me ka ʻōlelo
- Ka nānā ʻana i ke ʻano hoʻonui mua o ka pūnaewele hoʻomaka transcription
- RACE (ka hoʻonui wikiwiki o ka cDNA pau)
- Hoʻonui RNA laina
- Hoailona Chip
Ka holo hana
Ka mālama ʻana a me ka mālama ʻana
1.Nā kūlana hoʻokele
ʻO ke kaʻina holoʻokoʻa o ka lawe ʻana i ka pahu hau haʻahaʻa haʻahaʻa, e hōʻoia i ka pahu i loko o ke kūlana <4 ℃.
2. Nā kūlana mālama
RT maʻalahiTMMālama ʻia au ma -20 ° C.E mālama i ka huahana i loko o ka pahu hau wela mau ma -20 ° C ma hope koke o ka loaʻa ʻana.Inā kūpono nā kūlana mālama, ʻaʻole e hoʻohaʻahaʻa ka huahana i kekahi hana i ka manawa o ka makahiki 1.