RT Easyᵀᴹ II Master Premix no ke kaula cDNA synthesis mua no ka PCR manawa maoli
ʻO ka wehewehe ʻana o ka pahu:
- ʻO ka ʻōnaehana transcription hoʻohuli maikaʻi, he 20 mau minuke wale nō e hoʻopau i ka synthesis o ka cDNA strand mua.
- Kiʻekiʻe-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
- He kiʻekiʻe ka wela o ka ʻōnaehana hoʻohuli, hiki ke kiʻekiʻe ke kiʻekiʻe o ka hopena o ka ʻōnaehana e like me 50 ℃, a he maikaʻi kāna hana hoʻohuli.
- ʻO nā laʻana RNA haumia (ka waiʻona a hiki i ka 60%, ka paʻakai guanidine a hiki i ka 750mM) hiki ke hoʻololi i ka hopena transcription.
- 2× RT OR-MaʻalahiTM Ua hoʻohui ʻo Mix i nā kumu mua transcription hoʻohuli ʻia (Random Primer, Oligo(dT)18 Primer).
Nā wehewehe kime
ʻO Foregene's 2× RT OR-EasyTMHiki i ka hui ke hoʻohui i ka cDNA kaula mua ma kahi wela a hiki i ka 50°C, he mea kūpono ia i ka hoʻihoʻi ʻana i ka hoʻololi ʻana o nā kumu hoʻohālike RNA paʻakikī lua.
Loaʻa i ka RNA i hoʻomaʻemaʻe ʻia i loko o ke keʻena keʻena i ka waiʻona a me nā koena paʻakai guanidine, he mau mea hoʻoneʻe ikaika i ka hapa nui o nā transcriptases hoʻohuli, e hopena i nā hopena transcription huli ʻole a i ʻole ka haʻahaʻa haʻahaʻa.Hōʻike ka Foregene 2 × RT Mix i ka hoʻomanawanui kiʻekiʻe loa i ka waiʻona a me ka paʻakai guanidine.ʻO ka hoʻomanawanui kiʻekiʻe loa i ka waiʻona ma nā laʻana RNA he 60%, a ʻo ka ʻae kiʻekiʻe loa i ka paʻakai guanidine he 750mM.Hiki ke hoʻohana ʻia ka RNA maʻemaʻe no ka hoʻohuli ʻana i ka transcription me Foregene 2 × RT OR-EasyTMHuikau.
2× RT OR-MaʻalahiTMʻO Mix kahi reagent transcription wikiwiki i hoʻomohala kūikawā no Real Time PCR.He kiʻekiʻe ka maikaʻi o kēia ʻōnaehana hoʻohuli a ʻaʻole pono e hoʻohui i nā kumu mua, a hiki ke hana i nā hopena hoʻololi hoʻohuli maikaʻi ma kahi liʻiliʻi o nā mamana RNA.ʻO ka ʻōnaehana hoʻololi kū hoʻokahi e maʻalahi ka pane ʻana o RT, wikiwiki a ʻoi aku ka maikaʻi.Hiki ke hoʻopau ʻia ka strand cDNA synthesis mua i 20 mau minuke.Hiki ke hoʻohana ʻia kēia huahana i hui pū me Foregene's fluorescent quantitative product Real Time PCR EasyTMe kiʻi i nā hopena hoʻokolohua kiʻekiʻe.
Nā kikoʻī
| RT maʻalahiTMII Master Premix no ka strand cDNA synthesis no ka PCR Real Time | |||
| Nā ʻāpana pahu | RT-01021 | RT-01022 | RT-01023 |
| 50 T (10μl pūnaewele) | 200 T(10μl pūnaewele) | 800 T (10μl pūnaewele) | |
| 2× RT OR-MaʻalahiTMHuikau | 0.25ml | 1ml | 1ml × 4 |
| RNase-Free ddH2O | 1.7ml | 1.7ml | 1.7ml × 3 |
| Papa kuhikuhi | 1 apana | 1 apana | 1 apana |
Nā hiʻohiʻona & pono
- ʻO ka ʻōnaehana transcription hoʻohuli maikaʻi, he 20 mau minuke wale nō e hoʻopau i ka synthesis o ka cDNA strand mua.
- Kiʻekiʻe-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
- He kiʻekiʻe ka wela o ka ʻōnaehana hoʻohuli, hiki ke kiʻekiʻe ke kiʻekiʻe o ka hopena o ka ʻōnaehana e like me 50 ℃, a he maikaʻi kāna hana hoʻohuli.
- ʻO nā laʻana RNA haumia (ka waiʻona a hiki i ka 60%, ka paʻakai guanidine a hiki i ka 750mM) hiki ke hoʻololi i ka hopena transcription.
- 2× RT OR-MaʻalahiTMUa hoʻohui ʻo Mix i nā kumu mua transcription hoʻohuli ʻia (Random Primer, Oligo(dT)18Primer).
Ke noi pahu
- Hoʻohana pololei ʻia i ka Real Time PCR no ka hoʻopaʻa ʻana i ka huaʻōlelo gene
- Hiki iā ia ke nānā wikiwiki a pololei i nā helu liʻiliʻi o RNA e like me nā maʻi RNA
- Hoʻololi i ka unuhi ʻana o nā mamana RNA me ka maʻiʻo GC kiʻekiʻe a i ʻole ka hoʻolālā lua paʻakikī
Ka holo hana
Ka mālama ʻana a me ka mālama ʻana
1. Nā kūlana hoʻokele
ʻO ke kaʻina holoʻokoʻa o ka lawe ʻana i ka pahu hau haʻahaʻa haʻahaʻa, e hōʻoia i ka pahu i loko o ke kūlana o <4 ° C.
2. Nā kūlana mālama
RT maʻalahiTMMālama ʻia ʻo II ma -20°C.E mālama i ka huahana i loko o ka pahu hau wela mau ma -20 ° C ma hope koke o ka loaʻa ʻana.Inā kūpono nā kūlana mālama, ʻaʻole e hoʻohaʻahaʻa ka huahana i kekahi hana i ka manawa o ka makahiki 1.
