RT Easy II(me gDNase) Master Premix no ke kaula mua cDNA synthesis no Real Time PCR me gDNase
ʻO ka wehewehe ʻana o ka pahu:
-Ka hiki ke hoʻopau i ka gDNA, hiki ke wehe i ka gDNA i ka template i loko o 2 mau minuke.
-Efficient reverse transcription system, he 15 minuke wale no e hoopau ai i ka synthesis o ka cDNA strand mua.
- Nā hiʻohiʻona paʻakikī: hiki ke hoʻohuli ʻia nā hiʻohiʻona me ka maʻiʻo GC kiʻekiʻe a me ka hoʻolālā lua paʻakikī me ka hana kiʻekiʻe.
-High-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
-ʻO ka ʻōnaehana transcription reverse he paʻa wela kiʻekiʻe, ʻo ka mahana hopena maikaʻi loa he 42℃, a loaʻa mau ka hana transcription hope maikaʻi ma 50℃.
Nā kikoʻī
25×20µl rxns,100×20µl rxns
RT maʻalahiTMʻO II (Me gDNase) kahi ʻōnaehana hoʻololi i hoʻomohala kūikawā no Real Time PCR e hoʻopau koke i ka ʻino DNA genomic.Hiki iā 5×gDNase Mix ke wehe koke i ke koena genome ma RNA ma 42°C no 2 mau minuke, me ka pale pono ʻana i ka hoʻopili ʻana o ka genome ma nā hopena qPCR.
5× RO-MaʻalahiTMLoaʻa i ka Mix ka Foregene Reverse Transcriptase i hoʻomohala kūikawā ʻia e Foregene, kahi ʻano hou o ka reverse transcriptase me ka RNA affinity ikaika.ʻO ka ʻōnaehana i hoʻopaʻa ʻia e hoʻolōʻihi i ka transcription reverse rate, a hiki ke hoʻopau ʻia ka strand cDNA synthesis mua ma 42°C i 15 mau minuke.
Hiki i ka ʻōnaehana palapala hoʻohuli o kēia kit ke mālama i ka hana maikaʻi ma 50°C.No laila, ʻoi aku ka maikaʻi no nā mamana RNA paʻakikī a hiki ke unuhi maʻalahi i nā mamana RNA me ka ʻike GC kiʻekiʻe a me ka hoʻolālā lua paʻakikī.
Nā ʻāpana pahu
| 5× gDNase Mix |
| 5× RO-MaʻalahiTMHuikau |
| RNase-Free ddH2O |
| Nā kuhikuhi |
Nā hiʻohiʻona & pono
-Ka hiki ke hoʻopau i ka gDNA, hiki ke wehe i ka gDNA i ka template i loko o 2 mau minuke.
-Efficient reverse transcription system, he 15 minuke wale no e hoopau ai i ka synthesis o ka cDNA strand mua.
- Nā hiʻohiʻona paʻakikī: hiki ke hoʻohuli ʻia nā hiʻohiʻona me ka maʻiʻo GC kiʻekiʻe a me ka hoʻolālā lua paʻakikī me ka hana kiʻekiʻe.
-High-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
-ʻO ka ʻōnaehana transcription reverse he paʻa wela kiʻekiʻe, ʻo ka mahana hopena maikaʻi loa he 42℃, a loaʻa mau ka hana transcription hope maikaʻi ma 50℃.
Ke noi pahu
Hoʻohana pololei ʻia i ka Real Time PCR no ka hoʻopaʻa ʻana i ka huaʻōlelo gene.
Hiki iā ia ke nānā wikiwiki a pololei i ka trace RNA e like me RNA virus.
Hoʻololi i ka unuhi ʻana o nā mamana RNA me ka maʻiʻo GC kiʻekiʻe a i ʻole ka hana lua paʻakikī.
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
Pono e mālama ʻia ka pahu ma -20°C.E mālama i ka huahana i loko o ka pahu hau wela mau ma -20 ° C ma hope koke o ka loaʻa ʻana.Inā kūpono nā kūlana mālama, ʻaʻole e hoʻohaʻahaʻa ka huahana i kekahi hana i ka manawa o ka makahiki 1.
