RT-PCR Easyᵀᴹ II (ʻElua ʻanuʻu)
ʻO ka wehewehe ʻana o ka pahu:
◮Hiki ke hoʻokō koke ʻia ka loiloi quantitative manawa maoli o RNA.
◮Ke hoʻohana nei ka pahu i kahi mea hoʻohālikelike Foregene reverse transcription reagent a me Foregene HotStar Taq DNA Polymerase i hui pū ʻia me kahi ʻōnaehana kū hoʻokahi e hoʻomaikaʻi maikaʻi i ka pono amplification a me ka kikoʻī o ka pane.
◮ ʻO ka ʻōnaehana hopena maikaʻi ʻoi aku ka nui o ka ʻike ʻana, ʻoi aku ka ikaika o ka wela, a ʻoi aku ka maikaʻi o ka hoʻomanawanui.
Nā kikoʻī
RT-PCR maʻalahiTM II(ʻelua ʻanuʻu) Loaʻa nā reagents a pau no ka RT-PCR ʻelua ʻanuʻu, e hui pū ana i ka hopena RT a me ka hopena PCR i ka pahu hoʻokahi, e hāʻawi ana i ka RT Mix a me ka PCR Mix.Hiki i ka RT Mix ʻokoʻa ke synthesize i ka cDNA first-strand kiʻekiʻe me ka maʻalahi a me ka maikaʻi. 2× PCRmaʻalahiTM HuikauʻOi aku loaʻa kiʻekiʻe-efficiency a kiko'ī amplification.ʻO ka hui pū ʻana o nā mea ʻelua e ʻoi aku ka maʻalahi o ka ʻike ʻana i ka gene i hoʻopaʻa ʻia.
Nā ʻāpana pahu
| RT-PCRmaʻalahiTM II (ʻelua ʻanuʻu) | ||
| Kiko maʻiʻo | RT-02021 | RT-02022 |
| 100T(20μlʻōnaehana) | 500T(20μlʻōnaehana) | |
| 2× RT maʻalahiTM Huikau | 1ml | 1.7ml×3 |
| 2× PCRmaʻalahiTM HuikauʻOi aku | 1ml | 1.7ml×3 |
| Puka Lahui (50μM) | 200μl | 1ml |
| Oligo(dT)18 Puna (50μM) | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Papa kuhikuhi | 1ʻāpana | 1ʻāpana |
Nā hiʻohiʻona & pono
-Hiki ke hoʻokō koke ʻia ka loiloi quantitative manawa maoli o RNA.
-Ke hoʻohana nei ka pahu i kahi mea hoʻohālikelike Foregene reverse transcription reagent a me Foregene HotStar Taq DNA Polymerase i hui pū ʻia me kahi ʻōnaehana kū hoʻokahi e hoʻomaikaʻi maikaʻi i ka pono amplification a me ka kikoʻī o ka pane.
-ʻO ka ʻōnaehana hopena maikaʻi ʻoi aku ka nui o ka ʻike ʻana, ʻoi aku ka ikaika o ka wela, a ʻoi aku ka maikaʻi o ka hoʻomanawanui.
Kaʻina hana
Ka mālama ʻana a me ka mālama ʻana
1.Nā kūlana hoʻokele
ʻO ke kaʻina holoʻokoʻa o ka lawe ʻana i ka pahu hau haʻahaʻa haʻahaʻa, e hōʻoia i ka pahu i loko o ke kūlana <4 °C.
2. Nā kūlana mālama
Mālama ʻia ka pahu i -20 ° C.E mālama i ka huahana i loko o ka pahu hau wela mau ma -20 ° C ma hope koke o ka loaʻa ʻana.Inā kūpono nā kūlana mālama,tʻAʻole e hoʻohaʻahaʻa ka huahana i kekahi hana i ka wā 1 makahiki.
