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Lnc-RT Heroᵀᴹ I(Me gDNase)(Super Premix no ke kaula cDNA synthesis mua mai lncRNA)
Super Premix no ka cDNA synthesis mua mai ka lncRNA
Cat.No.RTL-09098/09099
ʻO ka ʻōnaehana hoʻololi wikiwiki a paʻakikī loa no ka hana ʻana i ka cDNA strand mua mai lncRNA.
Wehe i ka gDNA i 2 mau minuke.
15 mau minuke e hoʻopau i ka synthesis o ke kaula cDNA mua
Pūnaehana transcription hoʻohuli kiʻekiʻe
ʻO ke kūpaʻa wela kiʻekiʻe, ʻo ka mahana o ka hopena maikaʻi loa ʻo 42 ℃, a loaʻa nō ka maikaʻi o ka hana transcription huli ʻana ma 50 ℃.
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RT Easyᵀᴹ I Master Premix no ka hoʻohui ʻana i ka cDNA strand mua
- ʻO ka ʻōnaehana transcription maikaʻi loa, he 25 mau minuke wale nō e hoʻopau i ka synthesis o ka cDNA strand mua.
- Kiʻekiʻe-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
- ʻO ka ʻōnaehana transcription reverse he kiʻekiʻe ka wela wela, hiki ke kiʻekiʻe ke kiʻekiʻe o ka hopena o ka ʻōnaehana e like me 50 ℃,a loaʻa iā ia ka maikaʻi o ka hoʻihoʻi ʻana i ka hana unuhi.
- ʻO nā laʻana RNA haumia (ka waiʻona a hiki i ka 45%, ka paʻakai guanidine a hiki i 750mM) hiki ke hoʻololi i ka hopena transcription.
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RT Easy II(me gDNase) Master Premix no ke kaula mua cDNA synthesis no Real Time PCR me gDNase
-Ka hiki ke hoʻopau i ka gDNA, hiki ke wehe i ka gDNA i ka template i loko o 2 mau minuke.
-Efficient reverse transcription system, he 15 minuke wale no e hoopau ai i ka synthesis o ka cDNA strand mua.
- Nā hiʻohiʻona paʻakikī: hiki ke hoʻohuli ʻia nā hiʻohiʻona me ka maʻiʻo GC kiʻekiʻe a me ka hoʻolālā lua paʻakikī me ka hana kiʻekiʻe.
-High-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
-ʻO ka ʻōnaehana transcription reverse he paʻa wela kiʻekiʻe, ʻo ka mahana hopena maikaʻi loa he 42℃, a loaʻa mau ka hana transcription hope maikaʻi ma 50℃.
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RT Easyᵀᴹ II Master Premix no ke kaula cDNA synthesis mua no ka PCR manawa maoli
- ʻO ka ʻōnaehana transcription hoʻohuli maikaʻi, he 20 mau minuke wale nō e hoʻopau i ka synthesis o ka cDNA strand mua.
- Kiʻekiʻe-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
- He kiʻekiʻe ka wela o ka ʻōnaehana hoʻohuli, hiki ke kiʻekiʻe ke kiʻekiʻe o ka hopena o ka ʻōnaehana e like me 50 ℃, a he maikaʻi kāna hana hoʻohuli.
- ʻO nā laʻana RNA haumia (ka waiʻona a hiki i ka 60%, ka paʻakai guanidine a hiki i ka 750mM) hiki ke hoʻololi i ka hopena transcription.
- 2× RT OR-MaʻalahiTM Ua hoʻohui ʻo Mix i nā kumu mua transcription hoʻohuli ʻia (Random Primer, Oligo(dT)18 Primer).
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