ʻO SARS-CoV-2 Variant Nucleic Acid Detection Kit III (Multiplex PCR Fluorescent Probe Method)-no ka ʻike ʻana i nā mutants Delta
ʻO ka wehewehe ʻana o ka pahu:
◮ PCR pololei:ʻAʻohe pono no ka pahu nucleic acid extaction kit a me System, hoʻopau i 96 specimen i 1 hola a i ʻole.
◮ Pono nā mea hana haʻahaʻa a me ka hoʻohana maʻalahi loa:Pono wale ka ʻōnaehana PCR Real-Time.
◮ LoD a me ka ʻike kiʻekiʻe:Hiki i ka ʻike ʻike ke piʻi i ka pae haʻahaʻa e like me 500 kope/ml.
Nā kikoʻī
48 Rxns/kit, 96 Rxns/kit
| ʻAʻole. | ʻāpana | Ka nui | Mea nui | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Mea hoʻokuʻu waika nucleic | 1.4 mL/ paipu | 2 paipu | 5.3 mL/'ōmole | 1 omole | Mea hoʻokuʻu |
| 2 | RNA pale | 53 μL / paipu | 1 paipu | 106 μL / paipu | 1 paipu | ʻO ka mea hoʻopaneʻe RNase |
| 3 | ʻOkoʻa SARS-CoV-2 hoʻonā hopena A | 800 μL / paipu | 1 paipu | 1600 μL / paipu | 1 paipu | Primer, probe, hoʻopaʻa pane, dNTP |
| 4 | ʻO ka hui ʻana o ka enzyme SARS-CoV-2 | 160 μL / paipu | 1 paipu | 320 μL / paipu | 1 paipu | Hoʻomaka wela Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 mana maikaʻi | 100 μL / paipu | 1 paipu | 100 μL / paipu | 1 paipu | ʻO ka plasmid recombinant i loaʻa ka ʻāpana pahuhopu, RNA |
| 6 | ʻO SARS-CoV-2 ka mana ʻino | 1200 μL / paipu | 1 paipu | 1200 μL / paipu | 1 paipu | TE pale |
Nā hiʻohiʻona a me nā pono:
ʻO ka wikiwiki a me ka maʻalahi: hoʻopau i ka hoʻokolohua ma kahi o 1 hola
Hōʻalo i ka nalowale o ka waika nucleic: ʻaʻohe pono no ka hoʻomaʻemaʻe nucleic acid
Ka palena ʻike haʻahaʻa a me ka ʻike kiʻekiʻe, ʻoi aku ka mea kūpono no ka ʻike wikiwiki nui
ʻO ke koi haʻahaʻa haʻahaʻa a me ka hoʻohana maʻalahi loa
Hiki i ka ʻike ʻike ke piʻi i ka pae haʻahaʻa loa e like me 500 kope/mL).
Pehea e ho ohana:
1. Mea hoʻohana pono
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
2. Ke kaʻina hana ʻike
Ka hana laʻana: Naopharyngeal a i ʻole oropharyngeal swab sample (1 wale nō ka hana ʻana)(E kali no 10 min)
Hoʻonui ʻia ka PCR(<=55min)
Kaʻina hana:
Waihona:
Hoʻopaʻa ʻia mai ka mālamalama a mālama ʻia ma -20±5 ℃;
E kūʻai ma 2-8 ℃ ma hope o ka wehe ʻana, hoʻohana i loko o 7 mau lā;pale i ka hoʻoheheʻe hou ʻana (<5 mau manawa)
Ola ola:1 makahiki
