ʻO kēia ka nānā ʻana o nā pilikia hiki iBuccalpulupulu/FTA card DNA extraction is helpful to your experiment. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali:Tech@foregene.com.

Paʻa ke kolamu hoʻomaʻemaʻe

Ma keia kit, i loko o ka genomic DNA extraction hana, ka hoʻomaʻemaʻe kolamu ua pololei adsorbed ma luna o ka hāpana enzymatic lysis hui me ka centrifugation kaʻanuʻu, a me ka hoʻomaʻemaʻe kolamu hiki ke ālai 'ia ma muli o incomplete enzymization a me kiʻekiʻe viscosity o ka hāpana.

ʻO nā kumu kūpono e like me kēia:

1. ʻAʻole i hoʻopau ʻia ka hoʻoheheʻe enzymatic o nā ʻano kiko.

Manaʻo Manaʻo: Hiki ke hoʻonui ʻia ka manawa hana hoʻohālike o Foregene Protease a i ʻole hiki ke lawe ʻia ka supernatant ma hope o ka centrifugation ma 12,000 rpm (~13,400× g) no 5 min.

2. ʻO ka hoʻohana nui ʻana i nā mea kikoo a i ʻole nā ​​kikoʻī nui.

Manaʻo: ʻoi aku ka maikaʻi ʻaʻole ma mua o 1Buccal swab i ka hāpana;inā ʻoi aku ka nui o ka hāpana, e hoʻonui i ke ʻano o Buffer ST1, Foregene Protease, hoʻopaʻa ST2 e like me ia.

3. He kiʻekiʻe loa ka laʻana viscosity.

Manaʻo: Hiki ke hoʻoheheʻe ʻia nā laʻana me 10 mM o Tris-HCl ma mua o ka unuhi ʻana i ka DNA genomic.

4. Ua omo ʻia nā ʻāpana o ke kāleka koko.

Manaʻo Manaʻo: Hiki ke hoʻolōʻihi ʻia ka manawa centrifugation transient o ka ʻanuʻu 6 o ke kiko koko (FTA Card).

Haʻahaʻa haʻahaʻa a ʻaʻohe DNA

Nui nā ʻano mea like ʻole e pili ana i ka hua DNA genomic, e like me ke kumu hoʻohālike, nā kūlana mālama laʻana, ka hoʻomākaukau ʻana i ka laʻana, manipulation, etc.

ʻAʻole hiki ke loaʻa ka DNA Genomic i ka wā o ka unuhi ʻana

ʻO nā kumu kūpono e like me kēia:

1. ʻO ka mālama pono ʻole ʻana i nā laʻana a i ʻole mālama ʻia no ka lōʻihi loa e alakaʻi i ka genomic DNA degradation.

Manaʻo Manaʻo: Pono e hoʻāʻo hou ʻia nā swabs waha, a ʻaʻole pono e hoʻohana i nā swabs i mālama ʻia no nā hana unuhi DNA genomic;Pono nā laʻana kiko koko e hōʻoia i ka maikaʻi o ka maikaʻi a ʻaʻole lōʻihi ka manawa mālama.

2. ʻAʻole hiki ke hoʻohana ʻia ka liʻiliʻi o ka ʻiʻo i ka unuhi ʻana o ka DNA genomic pili.

Manaʻo: E hahai i kabuccal swab sampling kuhikuhi i loko o ke alakaʻi hana, a holoi i nā manawa he nui i hiki i hiki ke hoʻopili ʻia nā sela i ka swab waha no ka unuhi ʻana i ka DNA genomic;no ka lawe ʻana i kahi laʻana koko, hiki ke hoʻonui ʻia ka wahi ʻoki koko.

3. ʻAʻole mālama pono ʻia ka Foregene Protease, ka hopena i ka emi ʻana o kāna hana a i ʻole ka hana ʻole.

Manaʻo: E hōʻoia i nā kūlana mālama oka Foregene Protease a hoʻololi paha me kahi Foregene Protease hou no ka hopena enzymatic.

4. He lōʻihi loa ka mālama pono ʻole ʻana o ka pahu a i ʻole ka manawa mālama ʻana, e hopena i ka hemahema o kekahi mau mea i loko o ka pahu.

Manaʻo: E kūʻai i kahi houBuccal kāʻei DNAkaawale kit no nā kaʻina hana pili.

5. ʻAʻole hoʻohui ʻo Buffer WB i ka ethanol piha.

Manaʻo Manaʻo: E hōʻoia i ka hoʻohui ʻana o WB i ka leo kūpono o ka ethanol piha.

6. ʻAʻole i hoʻohui pono ʻia ka eluent i ke kiʻi silicone.

Manaʻo: Hoʻohui i 65°CE hāʻule ka eluent mua i ka waena o ka membrane silicone a waiho i ka lumi wela no 5 min e hoʻonui i ka pono elution.

Low-hua mai genomic DNA kaawale

ʻO nā kumu kūpono e like me kēia:

1. ʻO ka mālama pono ʻole ʻana i nā laʻana a i ʻole mālama ʻia no ka lōʻihi loa e alakaʻi i ka genomic DNA degradation.

Manaʻo Manaʻo: ʻoi aku ka maikaʻi o ka hoʻāʻo hou ʻana i nā swabs waha, a ʻaʻole pono e hoʻohana ʻia nā swabs i mālama ʻia no ka unuhi ʻana i ka DNA genomic.

2. Inā he liʻiliʻi loa ka nui o ka laʻana kiko, e emi ana ka ʻike DNA genomic i unuhi ʻia.

Manaʻo Manaʻo: E hahai i nā ʻōlelo aʻoaʻo laʻana o ka waha i loko o ke alakaʻi hana, e holoi i nā manawa he nui i hiki ke hoʻopili ʻia nā cell i ka swab waha no ka unuhi ʻana i ka DNA genomic.

3. ʻAʻole mālama pono ʻia ka Foregene Protease, ka hopena i ka emi ʻana o kāna hana a i ʻole ka hana ʻole.

Manaʻo: E hōʻoia i nā kūlana mālama othe Foregene Protease a hoʻololi paha me kahi mea hou Foregene Protease no ka hopena enzymatic.

4. Nā pilikia eluent.

Manaʻo: E hoʻohana i ka Buffer EB no ka elution;inā hoʻohana ʻo ddH₂ʻO a i ʻole nā ​​eluent ʻē aʻe, e hōʻoia i ka pH o ka eluate ma waena o 7.0-8.5.

5. ʻAʻole i hoʻokomo pono ʻia ka ʻeluate i ka kulu.

Manaʻo: E hoʻohui i nā kulu eluent i ka waena o ka membrane silicone a waiho i ka lumi wela no 5 min e hoʻonui i ka pono elution.

6. He liʻiliʻi loa ka wai elution.

Manaʻo: E hoʻohana i ka eluent no ka elution DNA genomic e like me ka mea i koi ʻia ma nā kuhikuhi, ma ka liʻiliʻi loaaole emi ma mua o 15μl.

Lauwe ka maemae of DNA genomickaawale

Hiki i ka hoʻomaʻemaʻe DNA genomic haʻahaʻa ke alakaʻi i ka hopena a i ʻole nā ​​​​hopena maikaʻi ʻole o nā hoʻokolohua o lalo, e like me: ʻaʻole hiki ke ʻoki ʻia nā enzymes, ʻaʻole hiki i ka PCR ke loaʻa i ka ʻāpana gene o ka hoihoi, etc.

ʻO nā kumu kūpono e like me kēia:

1. Heteroprotein pollution, RNA pollution.

Nānā: ʻAʻole holoi ʻia ke kolamu hoʻomaʻemaʻe me ka hoʻohana ʻana i ka Buffer PW;ʻaʻole holoi ʻia ke kolamu hoʻomaʻemaʻe holoi Buffer PW me ka hoʻohana ʻana i ka wikiwiki centrifugal pololei.

Manaʻo: E hōʻoia ʻaʻohe ua i loko o ka supernatant ma mua o ka hoʻohui ʻana i ka ethanol;e holoi i ke kolamu hoʻomaʻemaʻe e like me nā kuhikuhi, a ʻaʻole hiki ke haʻalele ʻia kēia hana.

2. Impurity ion pollution.

Nānā: Ua kāpae ʻia a holoi ʻia paha ke kolamu hoʻomaʻemaʻe ʻo Buffer WB i hoʻokahi wale nō, e hopena i ke koena ionic contamination.

Manaʻo Manaʻo: E holoi pono i ka Buffer WB 2 manawa e like me ke kuhikuhi ʻana e wehe i nā koena ion e like me ka hiki.

3. Hoʻohaumia i ka enzyme RNA.

Nānā: Ua hoʻohui ʻia nā RNases haole i ka pale;ʻAʻole i pololei ka holoi ʻana i ka Buffer PW, e hopena i nā koena RNase, e pili ana i nā hana hoʻokolohua RNA i lalo, e like me ka transcription in vitro.

Manaʻo: Foregene series nucleic acidisolaHiki i nā kits ke wehe i ka RNA me ka hoʻohui ʻole o RNase,penei Buccal Swab/FTA Kāleka DNA Isolation Kitpono't hoʻohui RNase;e hahai i nā kuhikuhi no ka kolamu holoi holoi holoi Buffer PW, a ʻaʻole hiki ke haʻalele ʻia kēia hana.

4. Ke koena ethanol.

ʻIkepili: ʻAʻole i hana ʻo Buffer WB i ka centrifugation paipu ʻole ma hope o ka holoi ʻana i ke kolamu hoʻomaʻemaʻe.

Manaʻo Manaʻo: E hana i ka hana centrifugation paipu hakahaka kūpono e like me nā kuhikuhi.

5. ʻO nā mea haumia ʻē aʻe.

Ka nānā 'ana: 'A'ole mālama 'ia nā la'ana i mālama 'ia a i 'ole nā ​​la'ana kūikawā.

Manaʻo: E mālama mua i ka hāpana e like me ke kuhikuhi ʻana.