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  • Kiko Kino DNA Hue Iole Nā Kime Hoʻoheheʻe DNA Genomic mai ka huelo ʻiole

    Kiko Kino DNA Hue Iole Nā Kime Hoʻoheheʻe DNA Genomic mai ka huelo ʻiole

    ʻO ka pahu wikiwiki loa o ka honua no ka unuhi ʻana i ka DNA genomic huelo ʻiole, hiki ke unuhi i ka DNA genomic kiʻekiʻe mai ka huelo ʻiole i loko o 1 hola.

    ◮ʻAʻole hoʻohaumia ʻo RNase:ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka DNA genomic me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.

    ◮wikiwiki wikiwiki:ʻOi aku ka hana o Foregene Protease ma mua o nā protease like, a ʻeli koke i nā ʻano kikoo;He maʻalahi ka hana, a hiki ke hoʻopau i ka hana unuhi DNA genomic i loko o 20-80 mau minuke.

    ◮ʻoluʻolu:Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.

    ◮Palekana:ʻAʻole koi ʻia ka hoʻoheheʻe ʻana i nā mea hoʻonaninani.

    ◮kūlana kiʻekiʻe:ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.

    ◮Pūnaehana micro-elution:Hiki iā ia ke hoʻonui i ka manaʻo o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.

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