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ʻO ke kelepona pololei RT qPCR Kit—Taqman Direct Cell Lysis Cell Ready Hoʻokahi ʻanuʻu qRT-PCR Kits Probe
Cat.No.DRT-01021/01022
No ke kelepona pololei RT-qPCR e hoʻohana ana i ≤ 1000,000 pūnaewele,
a me ka hana ʻana i ka RT qPCR pololei mai nā cell me ka hoʻomaʻemaʻe RNA mua.
Hoʻopili pololeiʻia nā pūnaewele e hoʻokuʻu i ka RNA no RT-qPCR;ʻo ka ʻōnaehana hoʻomanawanui kiʻekiʻe ʻaʻole pono e hoʻomaʻemaʻe i ka RNA a hoʻohana pololei i nā lysates cell e like me nā hoʻohālike RNA no nā hopena RT.ʻO ka wikiwiki a me ka maʻalahi;kiʻekiʻe sensitivity, ikaika specificity, a me ka maikai paʻa.
◮Maʻalahi a maikaʻi: me ka ʻenehana Cell Direct RT, hiki ke loaʻa nā laʻana RNA i loko o 7 mau minuke.
◮He liʻiliʻi ka noi hāpana, hiki ke hoʻāʻo ʻia e like me ka 10 cell.
◮Ka hoʻokomo kiʻekiʻe: hiki iā ia ke ʻike koke i ka RNA i loko o nā pūnaewele i hoʻoulu ʻia ma nā papa 384, 96, 24, 12, 6-well.
◮Hiki i ka DNA Eraser ke wehe koke i nā genomes i hoʻokuʻu ʻia, e hōʻemi nui i ka hopena i nā hopena hoʻokolohua ma hope.
◮ʻOi aku ka maikaʻi o ka ʻōnaehana RT-PCR i ʻoi aku ka maikaʻi a me ka PCR i ʻoi aku ka maikaʻi, a ʻoi aku ke kūpaʻa ʻana i ka RT-qPCR reaction inhibitors.
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Pūnaewele Direct RT qPCR Kit—SYBR GREEN I Direct Cell Lysis Cell Ready Hoʻokahi ʻanuʻu qRT-PCR Kit
Cat.No.DRT-01011/01012
No ke kelepona pololei RT-qPCR e hoʻohana ana i 10-1,000,000 pūnaewele,
a me ka hana ʻana i ka RT-qPCR pololei mai nā cell me ka ʻole o ka hoʻomaʻemaʻe RNA ma mua.
Hoʻopili pololeiʻia nā pūnaewele e hoʻokuʻu i ka RNA no RT-qPCR;ʻo ka ʻōnaehana hoʻomanawanui kiʻekiʻe ʻaʻole pono e hoʻomaʻemaʻe i ka RNA a hoʻohana pololei i nā lysates cell e like me nā hoʻohālike RNA no nā hopena RT.ʻO ka wikiwiki a me ka maʻalahi;kiʻekiʻe sensitivity, ikaika specificity, a me ka maikai paʻa.
◮Maʻalahi a maikaʻi: me ka ʻenehana Cell Direct RT, hiki ke loaʻa nā laʻana RNA i loko o 7 mau minuke.
◮He liʻiliʻi ka noi hāpana, hiki ke hoʻāʻo ʻia e like me ka 10 cell.
◮Ka hoʻokomo kiʻekiʻe: hiki iā ia ke ʻike koke i ka RNA i loko o nā pūnaewele i hoʻoulu ʻia ma nā papa 384, 96, 24, 12, 6-well.
◮Hiki i ka DNA Eraser ke wehe koke i nā genomes i hoʻokuʻu ʻia, e hōʻemi nui i ka hopena i nā hopena hoʻokolohua ma hope.
◮ʻOi aku ka maikaʻi o ka ʻōnaehana RT-PCR i ʻoi aku ka maikaʻi a me ka PCR i ʻoi aku ka maikaʻi, a ʻoi aku ke kūpaʻa ʻana i ka RT-qPCR reaction inhibitors.
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Lnc-RT Heroᵀᴹ I(Me gDNase)(Super Premix no ke kaula cDNA synthesis mua mai lncRNA)
Super Premix no ka cDNA synthesis mua mai ka lncRNA
Cat.No.RTL-09098/09099
ʻO ka ʻōnaehana hoʻololi wikiwiki a paʻakikī loa no ka hana ʻana i ka cDNA strand mua mai lncRNA.
Wehe i ka gDNA i 2 mau minuke.
15 mau minuke e hoʻopau i ka synthesis o ke kaula cDNA mua
Pūnaehana transcription hoʻohuli kiʻekiʻe
ʻO ke kūpaʻa wela kiʻekiʻe, ʻo ka mahana o ka hopena maikaʻi loa ʻo 42 ℃, a loaʻa nō ka maikaʻi o ka hana transcription huli ʻana ma 50 ℃.
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Hui Pū ʻIa RNA Hui Pū ʻIa Hui Pū ʻIa Hoʻomaʻemaʻe RNA no nā mea kanu i waiwai i nā Polysaccharides a me nā Polyphenols
Cat.No.RE-05021/05022/05024
No ka hoʻomaʻemaʻe ʻana i ka RNA a pau mai nā laʻana mea kanu maʻamau i loaʻa nā polysaccharide kiʻekiʻe a me nā ʻāpana polyphenol.
E wehe koke i ka RNA piha kiʻekiʻe mai nā laʻana mea kanu me ka nui o nā polysaccharides a me nā polyphenols.
RNase-Free me ka hoʻohana ʻana i ke kolamu hoʻomaʻemaʻe DNA
Maʻalahi—ua pau nā hana a pau i ka wela lumi
Hiki ke hoʻopau i ka hana i loko o 30 mau minuke
Palekana—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik
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ʻO ka mea kanu Hui Pū ʻIa RNA Isolation Kit Huina RNA Purificaiton Kit no nā mea kanu haʻahaʻa i loko o nā Polysaccharides a me nā Polyphenols
Cat.No.RE-05011/05014
No ka hoʻomaʻemaʻe ʻana i ka RNA āpau mai nā laʻana mea kanu maʻamau i loaʻa nā polysaccharide haʻahaʻa a me nā ʻāpana polyphenol.
Wehe koke i ka RNA holoʻokoʻa kiʻekiʻe mai nā mea kanu me ka polysaccharide haʻahaʻa a me ka polyphenol content.
RNase-Free
Hoʻopau maikaʻi i ka DNA me ka hoʻohana ʻana i ke kolamu hoʻomaʻemaʻe DNA
Wehe i ka DNA me ka hoʻohui ʻole i ka DNase
Maʻalahi—ua pau nā hana a pau i ka wela lumi
Hiki ke hoʻopau i ka hana i loko o 30 mau minuke
Palekana—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik
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Mea Hoʻokaʻawale DNA Mea kanu Genomic Plant DNA Purification Kits Reagents Protocol
Cat.No.DE-06111/06112/06113
No ka hoʻomaʻemaʻe DNA genomic mai nā ʻano mea kanu like ʻole.
E hoʻomaʻemaʻe koke a loaʻa ka DNA genomic kiʻekiʻe mai nā laʻana mea kanu (me nā polysaccharides a me nā laʻana mea kanu polyphenol).
◮ʻAʻohe haumia RNase
◮wikiwiki wikiwiki
◮Maʻalahi: Hiki ke hoʻopau i ka hana hoʻomaʻemaʻe i 30 mau minuke.
◮ʻoluʻolu: ʻAʻole koi ʻia ka wela lumi, 4 ℃ centrifugation a me ka ethanol precipitation o DNA.
◮Palekana: ʻaʻole hoʻohana ʻia ka mea hoʻoponopono organik.
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Viral RNA Isolation Kit no Viral RNA Hoʻomaʻemaʻe mai Plasma, Serum, a me nā Laʻana ʻē aʻe
Cat.No.RE-02011/02014
No ka hoʻomaʻemaʻe ʻana i ka RNA viral mai ka plasma, serum, nā wai kino ʻole o ke kino, nā supernatants moʻomeheu cell.
Hoʻokaʻawale wikiwiki a hoʻomaʻemaʻe i ka RNA viral mai nā laʻana e like me ka plasma, serum, nā wai kino ʻole o ke kelepona a me nā supernatants moʻomeheu cell.
-ʻAʻole pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.ʻO ka pahu holoʻokoʻa he RNase-Free
-Maʻalahi—ua hoʻopau ʻia nā hana a pau ma ka lumi wela
-Fast—hiki ke hoʻopau i ka hana i loko o 20 mau minuke
-Ka hua RNA kiʻekiʻe: ʻO ke kolamu RNA-wale nō a me ke ʻano hoʻohālikelike hiki ke hoʻomaʻemaʻe pono i ka RNA
-Pale—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik
-ʻO ka nui o ka hoʻoheheʻe ʻana o ka laʻana-a hiki i 200μl nā laʻana hiki ke hana i kēlā me kēia manawa.
-Ke kūlana kiʻekiʻe-ʻo ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe loa, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā noi hoʻokolohua like ʻole.
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ʻO nā pahu hoʻomaʻemaʻe hoʻomaʻemaʻe hoʻomaʻemaʻe ʻana i ka DNA Viral a me RNA Extraction
Cat.No.DR-01011/01012/01013
No ka hoʻomaʻemaʻe ʻana i ka DNA / RNA viral mai ka plasma, serum, nā wai kino cell-free, supernatants cell-culture.
Hoʻokaʻawale a hoʻomaʻemaʻe koke i ka DNA virus a i ʻole RNA mai nā laʻana e like me ka plasma, serum, ka wai kino ʻole o ke kino a me ka supernatant moʻomeheu cell.
ʻAʻohe RNA degradation.ʻO ka pahu holoʻokoʻa he RNase-Free
Maʻalahi—ua pau nā hana a pau i ka wela lumi
ʻO ka wikiwiki-hiki ke hoʻopau i ka hana i loko o 20 mau minuke
ʻO ka hua RNA kiʻekiʻe: ʻO ke kolamu RNA-wale nō a me ke kumu kūʻokoʻa hiki ke hoʻomaʻemaʻe pono i ka RNA
Palekana—ʻaʻole hoʻohana ʻia ka mea hoʻoheheʻe organik
Hiki ke hana ʻia ka nui o ka laʻana i kēlā me kēia manawa.
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Kiko Hoʻokaʻawale DNA Tissue Nā Kiko Hoʻoemi DNA mai nā Laʻana Tissue holoholona
ʻO ka unuhi wikiwiki a me ka hoʻomaʻemaʻe ʻana i ka DNA genomic mai nā kumu he nui, e like me nā ʻiʻo holoholona, nā cell, etc.
◮wikiwiki wikiwiki:ʻoi aku ka ʻoi aku o ka ʻoihana ma mua o nā protease like a hoʻoheheʻe wikiwiki i nā mea kikoo;
◮maʻalahi:hiki ke hoʻopau i loko o 50 minuke.
◮ʻoluʻolu:Hana ʻia ma ka lumi wela.
◮Palekana:ʻaʻole hoʻohana ʻia ka mea hoʻonaninani organik.
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Koko DNA Mini Kit
E hoʻomaʻemaʻe koke i ka DNA genomic kiʻekiʻe mai ke koko anticoagulated (<1ml).
◮ʻAʻole hoʻohaumia ʻo RNase:ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka DNA genomic me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.
◮wikiwiki wikiwiki:ʻOi aku ka hana o Foregene Protease ma mua o nā protease like, a ʻeli koke i nā ʻano kikoo;He maʻalahi ka hana, a hiki ke hoʻopau i ka hana unuhi DNA genomic i loko o 20-80 mau minuke.
◮ʻoluʻolu:Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.
◮Palekana:ʻAʻole koi ʻia ka hoʻoheheʻe ʻana i nā mea hoʻonaninani.
◮kūlana kiʻekiʻe:ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.
◮Pūnaehana micro-elution:Hiki iā ia ke hoʻonui i ka manaʻo o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.
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ʻO nā pahu liʻiliʻi Plasmid maʻamau
E hoʻomaʻemaʻe wikiwiki i ka DNA plasmid kiʻekiʻe mai nā bacteria i hoʻololi ʻia no nā hoʻokolohua molecular biology maʻamau e like me ka hoʻololi ʻana a me ka digestion enzyme.
◮Wehe i ka RNA me ka hoʻohui ʻole i ka RNase
◮ʻoluʻolu—hana ʻia ka centrifugation ma ka lumi wela
◮wikiwiki- hiki ke hoʻopau i ka hana i loko o 20 mau minuke
◮Palekana—ʻaʻole i hoʻohana ʻia ka mea hoʻoponopono organik
◮Maʻemaʻe kiʻekiʻe—OD260/280≈1.7-1.9
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RT Easyᵀᴹ I Master Premix no ka hoʻohui ʻana i ka cDNA strand mua
- ʻO ka ʻōnaehana transcription maikaʻi loa, he 25 mau minuke wale nō e hoʻopau i ka synthesis o ka cDNA strand mua.
- Kiʻekiʻe-sensitivity reverse transcription system, pg-level templates hiki ke loaʻa i ka cDNA kiʻekiʻe.
- ʻO ka ʻōnaehana transcription reverse he kiʻekiʻe ka wela wela, hiki ke kiʻekiʻe ke kiʻekiʻe o ka hopena o ka ʻōnaehana e like me 50 ℃,a loaʻa iā ia ka maikaʻi o ka hoʻihoʻi ʻana i ka hana unuhi.
- ʻO nā laʻana RNA haumia (ka waiʻona a hiki i ka 45%, ka paʻakai guanidine a hiki i 750mM) hiki ke hoʻololi i ka hopena transcription.
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