Kāpae
I nā makahiki i hala iho nei, ua hoʻohuli ʻia nā vesicles extracellular (EVs) i ka manaʻo o ka poʻe ma ke ʻano he mea lapaʻau kūpono;akā naʻe, ʻaʻole i hōʻike ʻia ka hopena therapeutic o nā EV ma ka endometriosis.ʻO ka Endometriosis kahi maʻi gynecological maʻamau ʻole e pili ana i ka 10-15% o nā wahine o ka wā hānau keiki, e hana ana i nā ʻano hōʻailona like ʻole, ka hopena i ka emi ʻana o ka maikaʻi o ke ola a me kahi kaumaha nui.
ʻŌlelo Hoʻolauna
Ma Iulai 20, 2021, ua paʻi ka hui noiʻi o Professor Wang Guoyun mai ka Halemai Qilu o ke Kulanui ʻo Shandong i kahi pepa noiʻi i kapa ʻia ʻo "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis" ma nā Frontiers i ka immunology, i kūkākūkā i ka derivation o M1 macrophages.ʻO ke kūpono o nā nanovesicles (NVs) i ka mālama ʻana i ka endometriosis.
Ke hoʻohana nei kēia ʻatikala i ke ʻano extrusion mau no ka hoʻomākaukau ʻana i nā M1NV, a hoʻohana i ke ʻano moʻomeheu co-culture e aʻo i nā loli i ka angiogenesis, neʻe, hoʻouka a me nā hōʻailona ʻē aʻe o nā eutopic endometrial stromal cell (EM-ESCs) mai nā maʻi me ka endometriosis.I ka manawa like, ua hoʻokumu ʻia kahi ʻiole o ka endometriosis, a ua mālama ʻia nā ʻiole me nā PBS, MONV a i ʻole M1NV, e loiloi i ka pono a me ka palekana o M1NV i ka mālama ʻana i ka endometriosis.
Ua hōʻike nā hopena i ka in vitro M1NVs hiki ke hoʻopaʻa pololei a i ʻole ka hoʻoneʻe ʻana i ka neʻe ʻana a me ka hoʻouka ʻana o EM-ESC, a kāohi i ka angiogenesis.Ma ke kŘkohu iole, ua pale ka M1NV i ka hiki mai o ka endometriosis ma o ka M2 macrophage reprogramming me ka hana ole i ka poino kino.Hōʻike ia e hiki i nā M1NV ke pale pololei i ka hanana ʻana o ka endometriosis, a hiki ke pale ʻia e ka repolarizing ʻano macrophages M2 i ka ʻano M1.No laila, ʻo ka hoʻohana ʻana i nā M1NV he ala hou no ka mālama ʻana i ka endometriosis.
Kokua Foregene
Ma ke aʻo ʻana, no ka mea, ua hoʻomākaukau ʻia ʻo M1NV ma ke kaomi mau ʻana i nā macrophages M1, ua hoʻohana ka ʻatikala i ka qRT-PCR e ʻike i nā mea pro-inflammatory a me nā māka M1 macrophage iNOS, TNF-a a me IL-6 mRNA ma M1NV a me M1 macrophages.Nā pāʻani pili o ka hoʻololi.Ua hōʻike ʻia nā hopena i loaʻa i nā M1NV nā mea pro-inflammatory factor mRNA a me M1 macrophage markers, e hōʻike ana e hiki i nā M1NV ke mālama pono i nā hiʻohiʻona hana o nā cell M1.Hoʻohana kēia ʻano noiʻi i ka QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I o Foregene
Kelepona pololei RT-qPCR Kitkikoʻī

Nā hiʻohiʻona noiʻi:
 
1. Hoʻoponopono Gene a me ka nānā ʻana i ka ʻōlelo, hōʻoia i ka overexpression a i ʻole ka hopena interference, ka nānā ʻana i ka lāʻau lapaʻau, etc.;
2. Ka ʻike ʻana o nā ʻano ʻano o nā pūnaewele paʻakikī e hoʻoulu ʻia e like me nā pūnaewele mua, nā pūnaewele pū, a me nā pūnana aʻalolo;
3. ʻIke i ka mRNA i nā laʻana e like me nā exosome a me nā nanovesicles.
Nā hiʻohiʻona: