ʻOi aku ma mua o ka hapalua o 2022, e hele mai e ʻike i nā ʻatikala kiʻekiʻe i hoʻopuka ʻia e Foeregene!

01

IF:17.694

Paʻi ʻia e: The First Affiliated Hospital, Sun Yat-sen University

Maʻiʻo nui: Esophageal squamous cell carcinoma (ESCC) ʻo ia ka subtype nui o ka maʻi maʻi esophageal.He mea nui ka ʻike ʻana i nā pahuhopu therapeutic kūpono no ka mālama maikaʻi ʻana o ESCC.Ua hōʻike kekahi mau haʻawina e hiki i ka RNA mismodification ke hoʻoikaika i ka ʻōlelo a me ka unuhi ʻana o nā mRNA pili i ka maʻi kanesa, akā ʻaʻole maopopo ke ʻano molekala.Ua hōʻike ʻia kēia haʻawina ʻo ka tRNA m7G methyltransferase-related complex proteins METTL1 a me WDR4 i hoʻonui nui ʻia i nā kiko ESCC a ua kōkua i ka prognosis maikaʻi ʻole o ESCC.Mai ia manawa, ua hōʻoia ka METTL1 a me ka WDR4 e pili ana i nā ʻiole kumu hoʻohālike i ka hoʻonui ʻia ʻana o ka hana o tRNA m7G methyltransferase e alakaʻi i ka hoʻoponopono ʻana i ka hōʻike ʻana o kēia mau protein ʻelua, ka mea e hoʻonui ai i ka hoʻonui ʻana o nā cell ESCC.Hōʻike kēia haʻawina i kahi hana oncogenic koʻikoʻi o ka misregulated tRNA m7G hoʻololi i ESCC a hōʻike ʻia ʻo ka huli ʻana i ka METTL1 a me kāna mau ala hōʻailona lalo e hoʻohiki i nā pahuhopu therapeutic no ka maʻi ESCC.

Huahana kōkua:

ʻO ka ʻiole huelo pololei PCR Kit Foregene#TP-01333

loulou kumu:

https://www.nature.com/articles/s41467-022-29125-7

02

IF:14.903

Paʻi ʻia e: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau

Maʻiʻo nui:

Me ka hoʻomohala ʻana o ka ʻenehana synthetic, ua lilo ka mRNA i papa hou o nā lāʻau lapaʻau.Eia nō naʻe, paʻakikī ka loaʻa ʻana o ka hoʻopuka intracellular kūpono o nā lāʻau mRNA ma muli o ka naʻau o ka mRNA i RNases.Hāʻawi kēia haʻawina i kahi hoʻolālā hoʻopuka kūpono o nā nanostructure polyphenol-conjugated hiki ke hoʻopuka maikaʻi i ka IL-10 mRNA i vivo.Ma hope o ka hoʻohana ʻana i kēia nanostructure no ka hāʻawi ʻana i nā lāʻau mRNA, ua hoʻoponopono pono ʻia nā pae IL-10, me ka pale ʻana i ka hōʻike ʻana o nā mea inflammatory, ka hoʻoikaika ʻana i ka hoʻoponopono mucosal, ka pale ʻana i nā cell epithelial colonic mai apoptosis, a me ka pale ʻana i ka dextran sodium sulfate-induced acute and chronic small.

Huahana kōkua:

Animal Total RNA Isolation Kit Foregene#RE-03011

loulou kumu:

https://www.sciencedirect.com/science/article/pii/S2211383522001551#:~:text=The%20polyphenol-assisted%20IL-10%20mRNA%20delivery%20system%20with%20self-protection,dextran%20sulfate%20tduced%20sulfate%20l kumu hoʻohālike.%202.

03

IF:12.499

Paʻi ʻia e: Laboratory of Experimental Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University

Maʻiʻo nui:

ʻO ke koʻikoʻi oxidative o ka sperm kahi kumu nui e alakaʻi ai i ka male ʻole o ke kāne, no laila he koho kūpono a kūpono hoʻi e hoʻokumu i nā hiʻohiʻona holoholona male infertility ma o ka hoʻoponopono ʻana i ka ʻōnaehana antioxidant ma ke ʻano i manaʻo ʻia.Eia nō naʻe, ʻaʻole i wehewehe ʻia nā hana antioxidant pili i ka cell germ cell.Ua hōʻoia kēia haʻawina ʻo LanCL1 gene he ʻano antioxidant kikoʻī i loko o nā cell germ kāne, a ua hōʻemi ʻia a pili ʻia paha kona hōʻike ʻana me ka infertility kāne kāne.ʻO nā ʻiole kāne nele i ka LanCL1 gene hōʻike i ka pōʻino sperm oxidative a me ka hoʻopōʻino ʻana i ka momona.I ke kaʻina o ka hoʻokaʻawale ʻana o ka sperm cell cell, i ka manawa e puka ai ke ʻano o ka oxidation, transcription factor-specific protein 1 (SP1) e hoʻoponopono i ka hōʻike ʻana o ka gene LanCL1, a laila e kāohi ai i ke kaʻina hana o ka sperm.Hōʻike kēia haʻawina he kuleana koʻikoʻi ke ala SP1-LanCL1 i ka hoʻoponopono ʻana i ka homeostasis testicular a me ka ulu ʻana o nā kāne i hoʻopaʻa ʻia e ka redox balance, a ke manaʻo nei ua loaʻa i nā ʻiole i hoʻololi ʻia ʻo LanCL1 i nā noi maikaʻi e like me ke ʻano holoholona o ka male ʻole o ke kāne.

Huahana kōkua:

ʻO ka ʻiole DNA Mini Kit Foregene#DE-05211

loulou kumu:

http://europepmc.org/article/MED/35469022

Kiko PCR pololei huelo iole

◮ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.

◮He liʻiliʻi ka laʻana koi, lawe wale i hoʻokahi hua.

◮ʻAʻole pono nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

◮ ʻO ka ʻōnaehana PCR i hoʻopaʻa ʻia e hiki ai i ka PCR ke loaʻa i ka kikoʻī kiʻekiʻe a me ka hoʻomanawanui ʻoi aku ka ikaika i ka PCR reaction inhibitors.

Huina Hoʻokaʻawale RNA Holoholona

◮ʻAʻole pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.ʻO ka ʻōnaehana holoʻokoʻa he RNase-Free.

◮Hoʻopau maikaʻi i ka DNA me ka hoʻohana ʻana i ke kolamu hoʻomaʻemaʻe DNA.

◮Wehe i ka DNA me ka hoʻohui ʻole i ka DNase.

◮Maʻalahi—ua pau nā hana a pau i ka wela lumi.

◮Ka wikiwiki—hiki ke hoʻopau i ka hana i loko o 30 mau minuke.

◮Paleʻa—ʻaʻole hoʻohana ʻia ka mea hoʻoponopono kūlohelohe.

◮Maʻemaʻe kiʻekiʻe—OD260/280≈1.8-2.1.

Kiko Mini DNA huelo iole

◮ʻAʻole hoʻohaumia ʻo RNase:ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA mai ka DNA genomic me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hale hana mai ka haumia ʻana e ka exogenous RNase.

◮wikiwiki wikiwiki:ʻOi aku ka hana o Foregene Protease ma mua o nā protease like, a ʻeli koke i nā ʻāpana kiko;maʻalahi ka hana, a hiki ke hoʻopau ʻia ka hana unuhi DNA genomic i loko o 20-80 mau minuke.

◮ʻoluʻolu:Hana ʻia ka centrifugation ma ka lumi wela, a ʻaʻohe pono no ka 4 ° C haʻahaʻa haʻahaʻa centrifugation a i ʻole ethanol precipitation o DNA.

◮Palekana:ʻAʻole koi ʻia ka hoʻoheheʻe ʻana i nā mea hoʻoheheʻe organik.

◮kūlana kiʻekiʻe:ʻO ka DNA genomic i unuhi ʻia he mau ʻāpana nui, ʻaʻohe RNA, ʻaʻohe RNase, a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.

◮Pūnaehana micro-elution:Hiki iā ia ke hoʻonui i ka manaʻo o ka genomic DNA, kahi kūpono no ka ʻike ʻana i lalo a i ʻole ka hoʻokolohua.