Nā hiʻohiʻona lncRNA:

1. ʻOi aku ka lōʻihi o nā lncRNA, me ka hōʻike ikaika a me nā ʻano hana splicing ʻokoʻa i ka wā o ka hoʻokaʻawale ʻana;

2. Hoʻohālikelike ʻia me nā genes coding, ʻoi aku ka haʻahaʻa o ka lncRNA;

3. Loaʻa i ka hapa nui o nā lncRNA ke ʻano kikoʻī o ke kino a me ka spatial i ke kaʻina o ka ʻokoʻa a me ka hoʻomohala ʻana;

4. Aia nā hiʻohiʻona o nā maʻi koko a me nā maʻi ʻē aʻe;

5. He okoa na wahi subcellular o lncRNA;

6. He haʻahaʻa ke kaʻina i ka mālama ʻana, akā aia ka hana i kahi pae o ka mālama ʻana a pēlā aku.

LncRNA hoʻololi unuhi unuhi:

Ua ʻike mākou he ʻano kūlana kiʻekiʻe ka lncRNA i nā cell.He mau hale paʻakikī kēia RNA e like me nā puka lou a i ʻole nā ​​lauoho lauoho.Inā ʻaʻole hiki ke hoʻoponopono maikaʻi ʻia ke ʻano lua o ka lncRNA, ʻaʻole i kūleʻa a kūleʻa paha i ka hana ʻana i ka RT-qPCR.ʻO ka pilikia o ka hana haʻahaʻa.

ʻO ka maʻamau, ʻekolu mau kumu nui no mākou e loaʻa ai nā template cDNA kiʻekiʻe:

ʻO ka maikaʻi o ka template RNA, nā kumu mua o ka transcription a me ka reverse transcriptase, a ʻo ka reverse transcriptase ke kumu nui.ʻO ka RNA i hoʻomaʻemaʻe ʻia e ka RNA extraction reagents i hoʻohana pinepine ʻia i nā koena o ka waiʻona a me nā paʻakai guanidine, he mau hopena pale ikaika i ka hapa nui o nā transcriptases hoʻohuli;inā he haʻahaʻa ka mahana maikaʻi loa no ka hopena transcriptase hope, ʻaʻole ia e kūpono i ka RNA.ʻO ka wehe ʻana o ke ʻano hierarchical.

I kēia manawa, ʻo ka mahana kūpono no ka reverse transcriptase i hoʻohana ʻia i ka hapa nui o nā laboratories ma ke ʻano he 42 ° C, a hiki i ka wela kiʻekiʻe ke hiki ma kahi o 50 ° C.ʻO ka ʻoiaʻiʻo, ʻo ka wehe ʻana o ka hoʻolālā RNA kiʻekiʻe e koi i kahi mahana kiʻekiʻe.

ʻO ka mahana no ka synthesizing i ka cDNA mua-strand o Fortune Biotech 2 × RT EasyTM Mix he kiʻekiʻe e like me 50 ℃-65 ℃, kahi kūpono e wehe pono ai i ke ʻano kiʻekiʻe o ka lncRNA, a pōkole ka manawa hana.

Eia kekahi, ua loaʻa i ka huahana ka mana anti-interference ikaika no ka RNA me ke koena o ka waiʻona a me ka paʻakai guanidine, hiki ke hoʻokō piha i nā pono o nā laboratories e hoʻokō ai i ka pono transcription kiʻekiʻe.

ʻO First Strand cDNA Synthesis Kit

'ikamu helu: RT-01011 (25 manawa)

Nā hiʻohiʻona:

1. ʻO ke kūpaʻa wela: 65 ℃ maikaʻi nō ka hana transcription hoʻohuli, e kōkua ana i ka wehe ʻana i ka hana lua paʻakikī.

2. ʻO ka hoʻomanawanui: hiki ke hana maikaʻi ʻia ka cDNA synthesis ma nā hoʻohālike RNA me ka maʻemaʻe maikaʻi ʻole (nā koena paʻakai ethanol a me guanidine)

3. ʻO ka wikiwiki: ka hana maʻalahi, hiki ke hoʻopau i ka synthesis cDNA mua i loko o 25-35 mau minuke

Nā noi lalo: PCR, qPCR, RACE, hōʻailona chip, kūkulu hale waihona, etc.

Eia kekahi, hāʻawi ʻo FJ Biotech i kahi pūʻulu piha o nā ʻōnaehana RT-PCR no ka hoʻokō ʻana i nā pono o nā ʻano mea kūʻai aku, me nā kits ma lalo nei:

ʻO First Strand cDNA Synthesis Kit (No qPCR)

'ikamu helu: RT-01021 (50 manawa)

Nā hiʻohiʻona:

1. ʻoluʻolu: Ua hoʻohui ʻia nā primer transcription hoʻohuli i mea e maʻalahi ai ka hana

2. ʻO ke kūpaʻa wela: 65 ℃ maikaʻi nō ka hana transcription hoʻohuli, e kōkua ana i ka wehe ʻana i ka hana lua paʻakikī.

3. ʻO ka hoʻomanawanui: hiki ke hoʻokō pono ʻia ka cDNA synthesis ma nā ʻano RNA me ka maʻemaʻe maikaʻi ʻole.

4. Naʻauao: kūpono no nā hōʻailona RNA haʻahaʻa haʻahaʻa

Ke noi haʻahaʻa: Hāʻawi kūikawā ʻia no qPCR ma hope

Hoʻokahi ʻanuʻu RT-PCR kit

'ikamu helu: RT-02011 (100 manawa)

Nā hiʻohiʻona:

1. ʻO ke kūpaʻa wela: 65 ℃ maikaʻi nō ka hana transcription hoʻohuli, e kōkua ana i ka wehe ʻana i ka hana lua paʻakikī.

2. Hoʻomanawanui: hiki ke hoʻokō pono ʻia ka cDNA synthesis ma nā ʻano RNA me ka maʻemaʻe maikaʻi ʻole.

3. Specificity: Hoʻomaikaʻi maikaʻi ka PCR MIX i hoʻolako ʻia i ka pono amplification a me ka kikoʻī o ka pane.

Nā noi haʻahaʻa: cDNA cloning, hoʻāʻo genetic a me nā hoʻokolohua molecular ʻē aʻe

ʻElua mau ʻanuʻu RT-PCR kit

'ikamu helu: RT-02021 (100 manawa)

Nā hiʻohiʻona:

1. Maʻalahi a me ka wikiwiki: hoʻokahi-tube pane

2. ʻO ke kūpaʻa wela: 65 ℃ maikaʻi nō ka hana transcription hoʻohuli, e kōkua ana i ka wehe ʻana i ka hana lua paʻakikī.

3. Specificity: ʻO ka Foregene Taq i hoʻohui ʻia e hoʻomaikaʻi maikaʻi i ka pono amplification a me ka kikoʻī o ka pane.

Nā noi haʻahaʻa: nā hoʻokolohua molekula e like me ka hoʻāʻo genetic

Hoʻokahi ʻanuʻu RT-qPCR kit-SYBR Green I

'ikamu helu: RT-02111 (100 manawa)

Nā hiʻohiʻona:

1. Maʻalahi: hoʻokahi-pahu hopena

2. ʻO ka naʻau kiʻekiʻe: hiki ke ʻike i nā kope kope haʻahaʻa

3. Hoʻohālikelike: kūpono no ka nui o nā mea hana fluorescence quantitative

Nā noi haʻahaʻa: ʻike i nā ʻano laʻana RNA e like me nā cell a me nā maʻi

Hoʻokahi ʻanuʻu RT-qPCR kit-Taqman

'ikamu helu: RT-02131 (100 manawa)

Nā hiʻohiʻona:

1. ʻO ka wikiwiki: ʻike i ka gene i hoʻopaʻa ʻia

2. ʻO ka naʻau kiʻekiʻe: hiki ke ʻike i nā kope kope haʻahaʻa

3. Hoʻohālikelike: kūpono no ka nui o nā mea hana fluorescence quantitative

Nā noi haʻahaʻa: ʻike i nā ʻano laʻana RNA e like me nā cell a me nā maʻi
Hiki iā Forge Bioreverse Transcription Kit ke kōkua iā ʻoe e hoʻoponopono maikaʻi i ka pilikia o ka hoʻololi ʻana.Ma ka manawa like, hui pū ʻo ia me Forge Bio RNA Extraction Kit (hiki iā ia ke wehe pono i nā koena DNA) e hāʻawi iā ʻoe i kahi hopena hoʻokolohua RNA piha.