ʻO nā ʻano PCR a me ka hoʻohaumia ʻana o ka waika nucleic aerosol i loko o nā hale hana hoʻāʻo nucleic acid e like me nā ʻaoʻao ʻelua o ke kālā.Hiki iā mākou ke koho e loaʻa a ʻaʻole paha, akā ʻaʻole hiki iā mākou ke koho inā makemake mākou a hoʻolilo paha.

1. Ka nānā 'ana i ka mea wehe DNA

No ka hoʻokō ʻana i ka wehe ʻana o ka nucleic acid aerosol contamination, pono mua ka nānā ʻana i nā mea wehe DNA i hiki ke hoʻoneʻe i nā waikawa nucleic i loko o kahi kūlana wai.No ka mea ʻaʻole nui nā mea wehe DNA e hana maoli.No ke ʻano hoʻokolohua, e ʻoluʻolu e nānā i: ʻAʻole hiki i ka wehe ʻana o DNA ke "koki huna" o ke keʻena hana!

Ma keia hoao ana, 100 kope/μL (CT ma kahi o 31) o ka ASFV plasmid a me ka DNA remover i heluia e ka PCR kikohoʻe ua hui pu ia ma na puke like, a laila reacted i ka lumi wela no 10min, 20min a me 30min pakahi.Ma hope o ka lawe ʻana i ka waika nucleic, ua hoʻokō ʻia ka hoʻonui ʻana o qPCR.Ua hoʻohālikelike ʻia kahi mana maikaʻi i hui pū ʻia me ka plasmid a me ka wai.Ma muli o ka pau ʻole o ka hoʻokolohua i ka manawa like, aia paha kekahi ʻokoʻa ma waena o nā hopena, akā ʻaʻole pili ia i ka hopena o ka hoʻokolohua.I kēia manawa, ua loiloi au i 10 mau huahana wehe DNA pāʻoihana.ʻO nā huahana No. 1, No. 6 a me No. 8 wale nō e hiki ke hoʻohaʻahaʻa maikaʻi i ka DNA plasmid i ke kūlana wai.ʻAʻohe hopena o nā huahana ʻē aʻe.

Papa 1 Nucleic acid ka hopena o ka wehe 'ana i ka DNA i loa'a ma ke kū'ai 'ana

2. Ka ho'āʻo ʻana i ka hopena hoʻoneʻe DNA o nā disinfectants maʻamau

1. Ka nānā ʻana i nā disinfectants maʻamau

Nui nā ʻano disinfectant i hoʻohana mau ʻia i loko o nā hale hana: aldehydes, phenols, alcohols, quaternary ammonium salts, peroxides, chlorine preparations, a me nā waikawa a me nā kumu.Ua hōʻoia piha ʻia nā hopena disinfection o kēia mau disinfectants ma nā microorganism e nā hoʻokolohua, akā ʻaʻole lawa ka ʻikepili hoʻokolohua e pili ana i ka hopena degradation o nā waikawa nucleic.Pono nā laboratories hoʻāʻo nucleic acid i kahi disinfectant ʻaʻole wale ka hopena disinfection maikaʻi i nā microorganism, akā hiki ke hoʻohaʻahaʻa i ka DNA.Hōʻike nā hopena hōʻike ʻelua wale nō hoʻomākaukau chlorine o 84 disinfectant a me trichloroisocyanuric acid a me 1 M hydrochloric acid e hoʻokō i nā koi.No ka mea ʻoi loa ka ʻino o ka waika hydrochloric 1 M, ua ʻōlelo ʻia e hoʻohana ʻia nā hoʻomākaukau chlorine e like me ka hoʻolei ʻōpala i nā keʻena hoʻāʻo nucleic acid.Eia nō naʻe, hiki ke hoʻomākaukau ʻia ka chlorine i nā metala a ʻaʻole hiki ke hoʻohana ʻia no ka disinfection o nā mea kani a me nā mea hana.

Papa 2 Nā hopena wehe DNA o nā ʻano disinfectants like ʻole

2. ʻO ka haʻahaʻa haʻahaʻa kūpono o ka hoʻomākaukau ʻana o ka chlorine

Loaʻa i nā disinfectants ma muli o ka chlorine ka hopena degradation DNA, akā ma muli o kā lākou metala corrosiveness a me ka huhū, hiki ke hoʻohana ʻia e hoʻomaʻemaʻe i ka papahele, nā countertops non-metal, nā ʻōlelo aʻoaʻo, nā paipu centrifuge a me nā mea hoʻāʻo ʻē aʻe.

Wahi a ka "New Coronary Pneumonia Epidemic Disinfection Technical Guidelines": ʻO nā mea hoʻohaumia (koko o nā maʻi, huna a me ka luaʻi) loaʻa ka chlorine 5g/L-10g/L chlorine-containing disinfectant;nā papahele, nā paia, a me nā ʻili mea e hoʻohana i ka chlorine i loaʻa 1g/L Chlorine disinfectant: hoʻomoʻi mua ʻia nā lole, nā moena a me nā lole ʻē aʻe i loko o ka chlorine disinfectant me ka 0.5g/L chlorine i loaʻa no 30 mau minuke, a laila hoʻomaʻemaʻe e like me ka maʻamau.

Papa 3 Ka hopena o ka wehe ʻana o DNA o nā ʻano ʻokoʻa o ka chlorine disinfectant

Hōʻike nā hopena hōʻike: inā ʻoi aku ka nui o ka chlorine ʻoi aku ka nui a i ʻole like me 1.2 g/L, hiki i ka disinfectant i loko o ka chlorine ke hoʻohaʻahaʻa loa i 100 kope/μL plasmid ke hoʻohana ʻia no 5 mau minuke.I ka hana ʻana no 10 mau minuke, hiki i ka 84 disinfectant me ka ʻoi aku ka nui o ka chlorine ma mua a i ʻole like me 0.6 g/L hiki ke hoʻohaʻahaʻa loa i 100 kope/μL o nā plasmids.

3. ʻO ka hoʻokolohua hoʻoneʻe ea i haumia e ka nucleic acid aerosol

Pehea e hoʻomaʻemaʻe ai i ka ea i haumia e ka nucleic acid aerosol?ʻO ka hapa nui o nā manaʻo e hana i kahi kaiapuni aerosol a laila hoʻomaʻemaʻe, a i ʻole e hoʻomaʻemaʻe i nā waikawa nucleic ma hope o ka haumia.Noʻu, he mea ʻole kahi hoʻokolohua ʻaʻole hiki ke hana hou ʻia a ʻaʻole hiki ke nānā ʻia nā hopena o ka hoʻokolohua, no laila ua ʻaiʻē au i kekahi mau ʻano o ka disinfectant air disinfection.E ʻike i ka "GB27948-2020 Nā Koina Nui no nā Air Disinfectants" a me "Disinfection Technical Specifications"

1. Nā mea ho'āʻo

1.1 Mea wehe DNA: Ma kēia hoʻokolohua, ua koho ʻia ʻelua mau mea hoʻoneʻe DNA i loaʻa ma ke kūʻai aku ʻo 6 a me 8 ma ka Papa 1.

1.2 Ho'āʻo plasmid: Ke noʻonoʻo nei e ʻoi aku ka kiʻekiʻe o ka helu CT o ka nui o ka pollution hale hana ma mua o 30, ʻo ka ʻike o ka ASFV gene plasmid i hoʻohana ʻia i kēia manawa ma kahi o 100 kope/μL, a ʻo ka fluorescent quantitative PCR CT ma kahi o 31.07.E hoʻohui i ka plasmid i ka pulupulu pulupulu a maloʻo ka ea.

1.3 Mea ho'āʻo: ʻO ka Bilingkehan KVBOX disinfection mea kani, i kākoʻo maikaʻi ʻia e Shenzhen Runlian Environmental Technology Co., Ltd., he hana maikaʻi loa ka disinfection ea me ka hydrogen peroxide disinfectant, a hiki ke pepehi pono i nā spores a me nā microorganism ʻē aʻe.

1.4 Wahi hoʻāʻo: kahi puka aniani hoʻololi i pani ʻia ma kahi o 0.1 cubic mika.

2. Keʻano ho'āʻo

ʻO ka nui o ka disinfectant i hoʻonohonoho ʻia ma ka mīkini disinfection he 10ml, a ʻo ka neʻe ʻana he 100ml/m3, ʻoi aku ka kiʻekiʻe ma mua o "GB27948-2020 Nā Manaʻo Nui no nā Air Disinfectants": i ka wā e hoʻohana ai i ka aerosol spray disinfection, ʻo ka nui o ka disinfectant e ≤10 ml / m3