Pahu hoʻokaʻawale RNA Total holoholona

Pahu hoʻokaʻawale RNA Total holoholona

Pahu Wehewehe:

Nā Paʻa Kit:

Pahu RL1, Pahu RL2 

Buffer RW1, Buffer RW2

RNase-Free ddH2E, Kolamu Hoʻomaʻemaʻe DNA

Kolamu RNA-Wale

Hoakaka:

50 Preps, 200 Preps


ʻIke kikoʻī

Nā huahana huahana

Nā Kūkākūkā Kits

Hoʻohana kēia kit i ka kolamu wili a me ka haʻilula i hoʻomohala ʻia e kā mākou ʻoihana, hiki ke unuhi i ka piha piha piha piha me ka kiʻekiʻe kiʻekiʻe RNA mai nā ʻano holoholona like ʻole me ka maikaʻi kiʻekiʻe. Hāʻawi ia i kahi kolamu hoʻomaʻemaʻe DNA, hiki ke hoʻokaʻawale maʻalahi a hoʻolaha i ka genomic DNA. mai ka supernatant a me ka lysateʻaʻaʻa, maʻalahi a mālama manawa; Hiki i ke kolamu RNA wale ke hoʻopaʻa pono iā RNA a hiki ke hana ʻia i ka manawa like me kahi ʻano ʻokoʻa he nui o nā laʻana.

ʻO RNase-Free ka ʻōnaehana holoʻokoʻa, no laila ʻaʻole e hoʻohaʻahaʻa ʻia ka RNA i lawe ʻia; ʻO Buffer RW1, ʻōnaehana holoi pale buffer RW2, no ka mea i loaʻa ʻole ka RNA i loaʻa ʻia i ka protein, DNA, ion, a me nā hoʻohaumia pūhui meaola.

Hiʻona & pono

■ ʻAʻole pono e hopohopo e pili ana i ka hoʻohaʻahaʻa RNA; ʻo RNase-Free ka ʻōnaehana holoʻokoʻa
■ E hoʻoneʻe kūpono i ka hoʻohana ʻana o DNA i kolamu hoʻomaʻemaʻe
■ Wehe i ka DNA me ka hoʻohui ʻole ʻana iā DNase
■ Hoʻopau nā hana āpau i ka mehana o ka lumi
■ Hiki ke hoʻopau i ka hana i 30 mau minuke
■ Palekana-ʻaʻohe meaola ʻona e koi ʻia
■ Maʻemaʻe kiʻekiʻe -OD260 / 280≈1.8-2.1

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Palapala noi kini

Kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe o ka RNA huina mai nā ʻano meaola holoholona hou a manuahi paha a i ʻole nā ​​ʻili moʻomeheu.

Nā palena huahana

■ Nā noi i lalo: ka syntandy cDNA mua-pae, RT-PCR, ka pākili mole, Northern Blot, etc.
■ Nā laʻana: nā meaola holoholona, ​​nā pūnaewele i hoʻomeheu ʻia
■ Nā hana: Nā Kiʻi 10-20mg, Nā Keʻena (2-5) × 106
■ Ka mana paʻa DNA nui o ke kolamu hoʻomaʻemaʻe: 80 μg
■ Volume Volume: 50-200 μl

Kahe ka hana

animal total RNA-simple workflow

Kiʻikuhi

3 Animal Total RNA Isolation Kit7

Mālama ʻia ka holoholona huina RNA Isolation Kit iā 20mg
Eia kekahi laʻana o nā ʻiole hou, e lawe iā 5% huina huina RNA 1% agar

ʻO Glycogel electrophoresis
1: ʻāpana 2: hakuʻala
3: Ake 4: Puʻuwai

Ka mālama a me ke ola papa

Hiki ke mālama ʻia ke kini i 24 mau mahina ma ka mahana o ka lumi (15-25 ℃) a i ʻole 2-8 ℃ no ka manawa lōʻihi. Hiki ke mālama ʻia ʻo Buffer RL1 ma 4 ℃ no hoʻokahi mahina ma hope o ka hoʻohui ʻana iā β- mercaptoethanol (pono ʻole).


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