Ma muli o ke kumumanaʻo o "ka maikaʻi, nā lawelawe, ka hana a me ka ulu ʻana", ua loaʻa iā mākou nā hilinaʻi a me nā hoʻomaikaʻi mai ka hale kūʻai kūloko a me ka honua no 100% Original Factory China Nucleic Acid Extraction and Purification Reagents/Rna/DNA Isolation Kit, Aloha mākou i nā mea kūʻai hou a ʻelemakule e kamaʻilio mai iā mākou ma ke kelepona a hoʻouna mai iā mākou i nā nīnau ma ka leka uila no ka loaʻa ʻana o nā hopena hui lōʻihi.
Ke hoʻomau nei i ke kumumanaʻo o "ka maikaʻi, nā lawelawe, ka hana a me ka ulu ʻana", ua loaʻa iā mākou nā hilinaʻi a me nā hoʻomaikaʻi mai ka hale kūʻai hale a me ka honua noʻO ka pahu hoʻoheheʻe Kina, ʻO ka pahu hoʻoheheʻe nucleic acid, Loaʻa ka inoa maikaʻi o kā mākou huahana kūpono mai ka honua e like me kāna kumukūʻai hoʻokūkū nui a me kā mākou pono nui loa o ka lawelawe ma hope o ke kūʻai aku i nā mea kūʻai aku.

wehewehe

50 Mākaukau, 200 Mākaukau

Ke hoʻohana nei kēia pahu i ke kolamu spin a me ke ʻano i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA piha maʻemaʻe kiʻekiʻe a kiʻekiʻe mai nā cell i hoʻoulu ʻia ma nā papa 96, 24, 12, a me 6-well.

Hāʻawi ka pahu i kahi kolamu hoʻomaʻemaʻe DNA maikaʻi, hiki ke hoʻokaʻawale i ka supernatant a me ka cell lysate, hoʻopaʻa a wehe i ka DNA genomic.He maʻalahi ka hana a mālama manawa.

Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA me kahi ʻano kū hoʻokahi.Hiki ke hana ʻia ka nui o nā laʻana i ka manawa like.

Nā ʻāpana pahu

*E ʻoluʻolu e ʻaʻahu i nā mīkina lima a e hana i nā hana pale i ka wā o ka hana ʻana no ka mea aia ʻo Buffer cRL1 a me Buffer RW1 i nā paʻakai chaotropic hoʻonāukiuki.

Nā hiʻohiʻona & pono

■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.
■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.
■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.
■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka nui o ka RNA mai nā cell moʻomeheu i nā papa 96, 24, 12, a me 6-well.

Ka holo hana

Diagram

Ua mālama ʻia ke kiʻi pākaukau agarose gel o ka Cell Total RNA Isolation Kit i nā helu like ʻole o luna, 20μl volume elution, e lawe i ka 2μl i hoʻomaʻemaʻe ʻia i ka RNA 1%.

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ʻo Buffer cRL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho). E pili ana i ke kumumanaʻo o "ka maikaʻi, nā lawelawe, ka hana a me ka ulu ʻana", ua loaʻa iā mākou nā hilinaʻi a me nā mea kūʻai aku o ka honua no 100% Original Factory China Nucleic Acid Extraction and Purification Reagents / Rnaged / DNA iā mākou e hoʻouna mai iā mākou i ke kelepona hou a me ka mea kūʻai aku. nā nīnau ma ka leka uila no nā hui ʻoihana lōʻihi a loaʻa nā hopena like.
100% hale hana kumuʻO ka pahu hoʻoheheʻe Kina, ʻO ka pahu hoʻoheheʻe nucleic acid, Loaʻa ka inoa maikaʻi o kā mākou huahana kūpono mai ka honua e like me kāna kumukūʻai hoʻokūkū nui a me kā mākou pono nui loa o ka lawelawe ma hope o ke kūʻai aku i nā mea kūʻai aku.