Hoʻokumu ʻia ka 100bp DNA Ladder me 13 mau kaula pālua o ka DNA, kahi i pāpālua ʻia ka manaʻo o 200bp, 500bp, a me 1000bp, e kōkua ana e lanakila i ka pilikia o nā ʻāpana pōkole i nāwaliwali i ka mālamalama a paʻakikī ke ʻike ma waena o nā kaula.Ma luna o 1000bp, hoʻohui ʻia ʻekolu mau pūʻulu o 1200bp, 1500bp, a me 2000bp, hiki ke helu ʻia e like me ka nui o ka ʻāpana DNA ma waena o 1000-2000bp.

Aia kēia huahana i ka 1 × Loading Buffer, hiki iā ʻoe ke lawe pololei i ka 3-6μl no ka electrophoresis e like me nā pono o ka hoʻokolohua, maopopo ke kiʻi electrophoresis, kūpono a kūpono.

ʻO nā pūʻulu 13 o kēia huahana mai ka liʻiliʻi a i ka nui: 100bp, 200bp, 300bp, 400bp, 500bp, 600bp, 700bp, 800bp, 900bp, 1000bp, 1200bp, 1200bp, 1500bpInā he 5μl ka nui o ka hoʻouka ʻana, 200bp, 500bp, a me 1000bp mau pūna he 100ng;he 50ng na kaula i koe.