1kb DNA Ladder
ʻO ka wehewehe ʻana o ka pahu:
◮Aia ka 1Kb DNA Ladder i 13 mau kaula ʻelua o DNA, a ʻo ka ʻike pālua o 1kb a me 5kb he mea kōkua ia e ʻike i nā ʻāpana like ʻole.Ma lalo o 1kb, hoʻohui ʻia ʻekolu mau ʻāpana o 250bp, 500bp, a me 750bp, a hiki ke helu ʻia ka nui o ka ʻāpana pōkole ma muli o kēia.Hoʻokaʻawale ʻia ka ʻike ʻana o nā pūna ʻehā o 6kb, 7kb, 8kb, a me 10kb, he mea maikaʻi ia e loaʻa ai kahi hopena hoʻokaʻawale maikaʻi.
Nā wehewehe
Aia ka 1Kb DNA Ladder i 13 mau kaula ʻelua o DNA, a ʻo ka ʻike pālua o 1kb a me 5kb he mea kōkua ia e ʻike i nā ʻāpana like ʻole.Ma lalo o 1kb, hoʻohui ʻia ʻekolu mau ʻāpana o 250bp, 500bp, a me 750bp, a hiki ke helu ʻia ka nui o ka ʻāpana pōkole ma muli o kēia.Hoʻokaʻawale ʻia ka ʻike ʻana o nā pūna ʻehā o 6kb, 7kb, 8kb, a me 10kb, he mea maikaʻi ia e loaʻa ai kahi hopena hoʻokaʻawale maikaʻi.
Aia kēia huahana i ka 1 × Loading Buffer.Hiki iā ʻoe ke lawe i ka 2-6μl no ka electrophoresis pololei e like me nā pono o ka hoʻokolohua.ʻO ke kiʻi electrophoresis he akaka, kūpono a kūpono hoʻi.
ʻO nā pūna 13 o kēia huahana mai ka liʻiliʻi a i ka nui: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Inā he 5μl ka nui o ka hoʻouka ʻana, he 100ng ka 1kb a me 5kb band;6kb, 7kb, 8kb, a me 10kb pūna he 25ng;a o na kaula i koe he 50ng.
Nā kikoʻī
| DL-30011 | 50 Hoʻomākaukau (250μl) |
| DL-30012 | 200 Hoʻomākaukau (1ml) |
Nā ʻāpana pahu
ʻIke wai mālama
10mM Tris-HCl(pH 8.4) 10mM EDTA
0.02% BPB
5% glycerinum
Kūlana huahana
ʻO ka nui o ka huahana ma kahi o 130μg / ml
Electtropherogram
Nā kūlana mālama
E ʻoluʻolu e mālama i ka hola 4℃(E ʻoluʻolu e mālama ma -20℃no ka mālama lōʻihi)
