e hoʻokō i ka ʻaelike", e like me ka koi o ka mākeke, hui pū mai ka hoʻokūkū mākeke ma kona ʻano maikaʻi e like me ka hāʻawi ʻana i ke kākoʻo piha a maikaʻi loa i nā mea kūʻai aku e ʻae iā lākou e lanakila nui.ʻO ka ʻimi ʻana o ka ʻoihana, ʻo ia ka leʻaleʻa o nā mea kūʻai aku no Kina New Product Best Selling Viral DNA & Rna Extraction Kit, Ua hoʻonui mākou i kā mākou ʻoihana i Kelemānia, Turkey, Kanada, USA, Indonesia, India, Nigeria, Brazil a me kekahi mau wahi o ka honua.Ke hana ikaika nei mākou e lilo i mea hoʻolako honua maikaʻi loa.
e hoʻokō i ka ʻaelike", e like me ka koi o ka mākeke, hui pū mai ka hoʻokūkū mākeke ma kona ʻano maikaʻi e like me ka hāʻawi ʻana i ke kākoʻo piha a maikaʻi loa i nā mea kūʻai aku e ʻae iā lākou e lanakila nui.ʻO ka ʻimi ʻana o ka ʻoihana, ʻo ia ka leʻaleʻa o nā mea kūʻai akuKina Rna&DNA Extraction Kit a me Nucleic Acid Extraction, Ke kakali nei mākou i ka hoʻokumu ʻana i kahi pilina pili like me ʻoe e pili ana i kā mākou huahana kiʻekiʻe a me nā hopena, nā kumukūʻai kūpono a me ka lawelawe maikaʻi loa.Manaʻolana mākou e lawe mai kā mākou huahana iā ʻoe i kahi ʻike leʻaleʻa a lawe i kahi manaʻo o ka nani.
Nā Papa kuhikuhi:Mea Manaʻo Hui Pū ʻIa RNA Isolation Kit Plus

e hoʻokō i ka ʻaelike", e like me ka koi o ka mākeke, hui pū mai ka hoʻokūkū mākeke ma kona ʻano maikaʻi e like me ka hāʻawi ʻana i ke kākoʻo piha a maikaʻi loa i nā mea kūʻai aku e ʻae iā lākou e lanakila nui.ʻO ka ʻimi ʻana o ka ʻoihana, ʻo ia ka leʻaleʻa o nā mea kūʻai aku no Kina New Product Best Selling Viral DNA & Rna Extraction Kit, Ua hoʻonui mākou i kā mākou ʻoihana i Kelemānia, Turkey, Kanada, USA, Indonesia, India, Nigeria, Brazil a me kekahi mau wahi o ka honua.Ke hana ikaika nei mākou e lilo i mea hoʻolako honua maikaʻi loa.
ʻO Kina Hua Hou Kina Rna&DNA Extraction Kit a me Nucleic Acid Extraction, Ke kakali nei mākou i ka hoʻokumu ʻana i kahi pilina pili pono me ʻoe e pili ana i kā mākou huahana kiʻekiʻe a me nā hopena, nā kumukūʻai kūpono a me ka lawelawe maikaʻi loa.Manaʻolana mākou e lawe mai kā mākou huahana iā ʻoe i kahi ʻike leʻaleʻa a lawe i kahi manaʻo o ka nani.

Hoʻopili ʻia ke kolamu

Ma hope o ka hoʻopili ʻana o ke kolamu, hoʻemi ʻia ka hua RNA a i ʻole hiki ke hoʻomaʻemaʻe i ka RNA, a haʻahaʻa ka nui o ka RNA i loaʻa.

ʻIkepili kumu maʻamau:

1. ʻAʻole pololei nā haʻawina laʻana.

ʻAʻole hiki i ka haʻihaʻi laʻana ke hoʻopaʻa ʻia ka DNA-CLEANING COLUMN, ʻoiai e pili ana i ka hua RNA a me ka maikaʻi.Paipai mākou i ka hana wili wikiwiki i loko o ka wai naikokene lawa i ka wā i haki ai ʻoe i nā laʻana, E hoʻāʻo e ʻoki i ka pā hale hāpana, membrane cell a me nā mea ʻē aʻe.No nā laʻana mea kanu o polyol polysaccharides, paipai mākou iā ʻoe e hoʻohana i ka Plant Total RNA ISOLATION KIT PLUS.

2. Ke hoʻomoʻa ʻia ka laʻana supernatant i hoʻokaʻawale ʻia me ke kolamu hoʻomaʻemaʻe DNA, hiki ke hoʻomoʻa ʻia ka wai i ʻoki ʻia.

ʻO nā sediments ʻāpana pūlima i lawe ʻia e hoʻopau ʻia ke kolamu RNA-ONLY i ka wā e hana ʻia ai ka hana adsorption RNA (e nānā i ka pae 6).Paipai mākou iā ʻoe me ke akahele i ka wā e hoʻomohu ai i kēia supernatant e pale aku i nā ʻōpala o ke kelepona i ka omo ʻana.

3. He nui loa ka helu mua.

ʻO ka hoʻohana nui ʻana i ka laʻana e hopena i ka ʻāpana hāpana piha ʻole a i ʻole ka pau ʻole o ka cell lysis e Buffer PSL1, ka hopena o ka paʻa ʻana o ke kolamu hoʻomaʻemaʻe i ka wā hoʻomaʻemaʻe.He 50 mg ka mea kanu Total RNA Isolation Kit.No nā laʻana mea kanu o polyol polysaccharides, paipai mākou iā ʻoe e hoʻāʻo iā Plant Total RNA ISOLATION KIT PLUS.

4. He haʻahaʻa loa ka mahana o ka centrifuge.

Lawe ʻia ke kaʻina hoʻokaʻawale a me ka hoʻomaʻemaʻe RNA holoʻokoʻa ma ka lumi wela (20-25°C), koe naʻe ka haʻihaʻi ʻana o ka ʻiʻo hāpana e ka wai hau.℃, hiki ke hoʻopaʻa ʻia ke kolamu hoʻomaʻemaʻe DNA a/a i ʻole kolamu RNA wale nō.Inā hiki kēia, e hoʻonoho i ka mahana centrifuge i 20-25℃, ae hoʻomaʻamaʻa mua i ka hui ʻana o ka lysis a/a i ʻole ka supernatant i hoʻohui ʻia ethanol i 37°C.

ʻAʻohe RNA i unuhi ʻia a haʻahaʻa paha ka hua RNA

Nui nā kumu e pili ana i ka pono o ka hoʻihoʻi ʻana, e like me: sample RNA content, operation method, the elution volume, etc.

Ka nānā 'ana i nā kumu ma'amau e like me lalo:

1. Ua hana ʻia kahi ʻauʻau hau a i ʻole ka wela haʻahaʻa (4 ° C) centrifugation i ka wā o ka hana.

Manaʻo: E hana ma ka lumi wela (15-25°C) i ke kaʻina holoʻokoʻa, mai hana i ka hau hau a me ka haʻahaʻa wela centrifugation.

2. Ua hoʻohaʻahaʻa ʻia ka RNA ma muli o ka mālama pono ʻole ʻana o ka hāpana a i ʻole ka mālama lōʻihi o ka hāpana.

Manaʻo Manaʻo: Pono e hoʻopaʻa koke ʻia nā laʻana i hōʻiliʻili hou ʻia i loko o ka hau hau, a laila mālama ʻia ma -80 ° C no ka manawa lōʻihi, e pale i ka hoʻoheheʻe pinepine ʻana a me ka hoʻoheheʻe ʻana o nā laʻana;a i ʻole e hoʻoinu koke i nā laʻana i loko o ka solution RNA stabilizer RNAlater solution (nā laʻana holoholona).

3.Insufficient hāpana fragmentation a me lysis alakai i blockage o ke kolamu hoomaemae.

Manaʻo Manaʻo: I ka wā e wili ai i ka ʻiʻo, e ʻoluʻolu e hoʻopaʻa pono i ka lepo, a e hoʻoneʻe koke iā ia i ka Buffer PSL1 i hoʻomākaukau mua ʻia (e hōʻoia ua hoʻohui ʻia ka māhele kūpono o β-ME, e ʻike i ka pae 1 o ke kaʻina hana).

4. Ua hoʻohui hewa ʻia ka eluent.

Manaʻo Manaʻo: E hōʻoia i ka kulu ʻana o RNase-Free ddH2O i waenakonu o ka membrane kolamu hoʻomaʻemaʻe.

5. ʻAʻole i hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer PSL2 a i ʻole Buffer PRW2.

Manaʻo Manaʻo: E ʻoluʻolu e hahai i nā ʻōlelo kuhikuhi, e hoʻohui i ka leo kūpono o ka ethanol piha i ka Buffer PSL2 a me Buffer PRW2 a hui maikaʻi ma mua o ka hoʻohana ʻia ʻana o ka pahu.

6. ʻAʻole kūpono ka nui o nā kiko kiko.

Manaʻo Manaʻo: E hoʻohana i 50 mg o ka ʻiʻo no 500 μl o Buffer PSL1.ʻO ka hoʻohana ʻana i ka ʻiʻo nui e hōʻemi i ka nui o ka RNA i unuhi ʻia a e hōʻemi pū ʻia ka maʻemaʻe o ka RNA hopena.Manaʻo ikaika mākou ʻaʻole pono e ʻoi aku ka nui o ka hoʻohālike mua ma mua o 50 mg no ka hana unuhi RNA.

7.Inappropriate elution volume a incomplete elution.

Manaʻo: ʻO ka leo eluent o ke kolamu hoʻomaʻemaʻe he 50-200 μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono e hoʻolōʻihi i ka manawa ma ka lumi wela ma hope o ka hoʻohui ʻana i ka RNase-Free ddH2O preheated, e like me 5-10min.

8. He koena ethanol ka kolamu hoʻomaʻemaʻe ma hope o ka holoi ʻana me BufferPRW2.

Manaʻo Manaʻo: Inā centrifuged ka paipu ʻole no 1 min a aia nō ka ethanol i koe ma hope o ka holoi ʻana i Buffer PRW2, hiki iā ʻoe ke hoʻonui i ka manawa o ka centrifugation paipu ʻole i 2 min, a i ʻole e kau i ke kolamu hoʻomaʻemaʻe ma ka lumi wela no 5 min e wehe loa i ke koena ethanol.

9. Ua hoʻohana hewa ʻia ka pahu.

Manaʻo Manaʻo: No nā laʻana mea kanu o polyphenolic polysaccharides, me ka hoʻohana ʻana i nā pahu maʻamau e like me Plant Total RNA Isolation Kit ʻaʻole hiki ke loaʻa nā laʻana RNA kūpono.Paipai mākou iā ʻoe e hoʻohana i ka Plant Total RNA IsolationKit Plus, i hoʻolālā kūikawā ʻia no nā laʻana mea kanu polyphenolic polysaccharide.He kit i hoʻomohala kūikawā no ka unuhi ʻana i ka RNA mai nā laʻana mea kanu polyphenol a me polysaccharide.

He haʻahaʻa ka waiwai OD260/OD280

ʻO ka elution RNA me ddH2O a hoʻohana ʻia no ka heluhelu ʻana i ka spectrophotometer i loaʻa nā waiwai haʻahaʻa OD260/OD280.Manaʻo mākou e hoʻohana i ka 10 mM Tris-HCl, pH 7.5 (ma mua o ka RNase-Free ddH2O e hoʻoheheʻe i ka RNA) no ka loaʻa ʻana o nā koina OD260/OD280 pololei, e ʻike i ka "RNA Concentration and Purification Assays" ma ka ʻaoʻao 19.

Hoʻohaʻahaʻa ʻia ka RNA i hoʻomaʻemaʻe ʻia

ʻO ka maikaʻi o ka RNA i hoʻomaʻemaʻe ʻia e pili ana i nā mea e like me ka mālama ʻana i ka laʻana, ka haumia RNase, a me ka manipulation.

Ka nānā 'ana i nā kumu ma'amau:

1.ʻAʻole i mālamaʻia nā'āpana kiko i ka manawa ma hope o ka hōʻiliʻiliʻana.

Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia nā laʻana kikoʻī i ka manawa ma hope o ka hōʻiliʻili ʻana, e ʻoluʻolu e mālama iā lākou i loko o ka nitrogen wai i ka haʻahaʻa haʻahaʻa koke a i ʻole e hoʻololi iā lākou i -80°C no ka mālama lōʻihi ma hope o ka hoʻomaloʻo wikiwiki ʻana i loko o ka nitrogen wai, a i ʻole e hoʻokomo koke i nā laʻana i loko o ka RNA stabilizer RNAlater solution (nā mea hoʻohālike holoholona).No ka unuhi RNA, e ho'āʻo e hoʻohana i nā laʻana kikokikona i hōʻiliʻili hou ʻia.

2. Hoʻohou hou i ka maloʻo a me ka hoʻoheheʻe ʻana i nā ʻāpana kiko.

Manaʻo Manaʻo: I ka mālama ʻana i nā laʻana ʻiʻo, ʻoi aku ka maikaʻi e ʻoki i nā ʻāpana liʻiliʻi no ka mālama ʻana, a lawe i kahi ʻāpana o ia mau mea i ka wā e hoʻohana ai i mea e pale ai i ka hōʻino ʻana o ka RNA ma muli o ka hoʻomaloʻo pinepine ʻana a me ka hoʻoheheʻe ʻana o nā laʻana.

3. Hoʻokomo ʻia ʻo RNase i loko o ka lumi hana a ʻaʻole i hoʻohana ʻia i nā mīkina lima hoʻolele, masks, etc.

Manaʻo Manaʻo: ʻoi aku ka maikaʻi o nā hoʻokolohua RNA extraction i nā hana RNA kaʻawale, a pono e hoʻomaʻemaʻe ʻia ka papa ʻaina ma mua o ka hoʻokolohua, a pono e ʻaʻahu ʻia nā mīkina lima a me nā masks i ka wā o ka hoʻokolohua e pale aku ai i ka hōʻino ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase i ka nui loa.

4. Ua haumia ka reagent e RNase i ka wā e hoʻohana ai.

Manaʻo Manaʻo: E hoʻololi me kahi pūʻulu hou o nā pahu hōʻiliʻili RNA piha no nā hoʻokolohua pili.

5. ʻO nā paipu centrifuge a me nā ʻōlelo aʻoaʻo pipette i hoʻohana ʻia no ka hoʻoponopono RNA ua haumia me RNase.

Manaʻo Manaʻo: E hōʻoia i ka RNase-Free nā paipu centrifuge, nā ʻōlelo aʻoaʻo pipette, nā pipette, a me nā mea ʻē aʻe i hoʻohana ʻia i ka unuhi RNA.

Nā Papa kuhikuhi:

Mea Manaʻo Hui Pū ʻIa RNA Isolation Kit Plus