hale kūʻai kumu kūʻai haʻahaʻa Kina Long Taq DNA Polymerase, Long PCR
Ke hoʻohana nei i kahi ʻōnaehana hoʻokele maikaʻi maikaʻi ʻepekema, maikaʻi loa a me ka hilinaʻi kiʻekiʻe, lanakila mākou i ke kūlana maikaʻi a noho i kēia aʻo no ka hale kūʻai haʻahaʻa Kina Long Taq DNA Polymerase, Long PCR, Hoʻokipa maikaʻi mākou i nā malihini a pau e hoʻonohonoho i nā pilina ʻoihana me mākou e pili ana i ke kumu o nā ʻano maikaʻi.Pono ʻoe e kelepona mai iā mākou i kēia manawa.Hiki iā ʻoe ke loaʻa i kā mākou pane akamai i loko o 8 mau hola.
Ke hoʻohana nei i kahi ʻōnaehana hoʻokele maikaʻi ʻepekema piha, maikaʻi loa a me ka hilinaʻi kiʻekiʻe, lanakila mākou i ke kūlana maikaʻi a noho i kēia aʻo ʻana noHoʻonui ʻia PCR Kina, Qpcr, Hoʻopili kā mākou hui i kahi o 20, 000 square meters.Ua loaʻa iā mākou ma mua o 200 mau limahana, hui loea loea, 15 mau makahiki ʻike, hana nani, kūpaʻa a hilinaʻi maikaʻi, kumukūʻai hoʻokūkū a lawa ka mana hana, ʻo ia kā mākou e hoʻoikaika ai i kā mākou mea kūʻai aku.Inā he nīnau kāu, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou.
Nā kikoʻī
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Nā ʻāpana pahu
Mahele I | Hoʻopaʻa FP |
Foregene Protease | |
6× DNA Hoʻouka Pahu | |
Mahele II | 2×PCR maʻalahiTMHuikau |
Nā kuhikuhi |
Nā hiʻohiʻona & pono
■ ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.
■ He liʻiliʻi ke koi hoʻohālike, hiki ke hoʻāʻo ʻia e like me 1mg iʻa caudal fin a i ʻole 10 hua iʻa.
■ ʻAʻole koi ʻia nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.
■ ʻOi aku ka maikaʻi o ka PCR ʻoi aku ka maikaʻi o ka hoʻomanawanui ʻana i ka PCR reaction inhibitors.
Nā palena kime
Hoʻohana: ka iʻa Zebra a me nā iʻa wai hou ʻē aʻe.
Hoʻokuʻu ʻia ka DNA mai ka laʻana lysis: hoʻohana wale ʻia ma ke ʻano he PCR template.
Hiki ke hoʻohana ʻia ke kit no nā kumu penei: ʻike i ka transgenosis, genotyping holoholona, etc.
Ka holo hana
Ka mālama ʻana a me ka mālama ʻana
Pono e mālama ʻia ka ʻāpana I o kēia pahu ma 2-8 ℃.
Hiki ke mālama ʻia ka reagent Buffer FP no 12 mahina ma lalo o nā kūlana maloʻo;hiki ke mālama ʻia ma 2-8 ℃ no ka mālama lōʻihi.
v Loaʻa i ka Reagent Foregene Protease kahi kumu kūʻokoʻa.No ka hōʻoia ʻana i kāna hana a paʻa, e ʻoluʻolu e mālama iā ia ma 4 ℃ no 12 mahina.
vReagent 6×DNA Loading Buffer hiki ke mālama ʻia ma 4 ℃ a i ʻole -20 ℃ no ka manawa lōʻihi.
Pono e mālama ʻia ka ʻāpana II o kēia pahu ma -20 ℃.
vReagent 2×PCR Easy TM Mix, ina e hoʻohana pinepine, hiki ke mālama 'ia ma 4 ℃ no ka pōkole-manawa mālama (hoʻohana i loko o 10 lā) .Pono ʻoe e kelepona mai iā mākou i kēia manawa.Hiki iā ʻoe ke loaʻa i kā mākou pane akamai i loko o 8 mau hola.
kumu kūʻai haʻahaʻaHoʻonui ʻia PCR Kina, Qpcr, Hoʻopili kā mākou hui i kahi o 20, 000 square meters.Ua loaʻa iā mākou ma mua o 200 mau limahana, hui loea loea, 15 mau makahiki ʻike, hana nani, kūpaʻa a hilinaʻi maikaʻi, kumukūʻai hoʻokūkū a lawa ka mana hana, ʻo ia kā mākou e hoʻoikaika ai i kā mākou mea kūʻai aku.Inā he nīnau kāu, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou.