Hāʻawi mau mākou iā ʻoe i kekahi o nā mea kūʻai aku ʻoi loa, a me nā ʻano hoʻolālā ākea a me nā ʻano me nā mea maikaʻi loa.Aia kēia mau hana i ka loaʻa ʻana o nā hoʻolālā maʻamau me ka wikiwiki a me ka hoʻouna ʻana no Fixed Competitive Price Lab Consumables Universal Nucleic Acid Extraction Test Vials Kits, ʻO kā mākou huahana a me nā hoʻonā hauʻoli i ka kaulana kaulana i waena o kā mākou mea kūʻai.Hoʻokipa mākou i nā manaʻo, nā hui hui a me nā hoaaloha pili mai nā wahi āpau o kou honua e launa pū me mākou a ʻimi i ka launa pū ʻana no ka loaʻa ʻana o kekahi.
Hāʻawi mau mākou iā ʻoe i kekahi o nā mea kūʻai aku ʻoi loa, a me nā ʻano hoʻolālā ākea a me nā ʻano me nā mea maikaʻi loa.Loaʻa kēia mau hana i ka loaʻa ʻana o nā hoʻolālā maʻamau me ka wikiwiki a me ka hoʻouna ʻana noKina Universal Nucleic Acid Extraction a me Nucleic Acid Extraction Extraction, 'O kēlā me kēia mea kū'ai'olu'olu kā mākou pahuhopu.Ke ʻimi nei mākou i ka launa pū ʻana me kēlā me kēia mea kūʻai aku.No ka hoʻokō ʻana i kēia, mālama mākou i kā mākou maikaʻi a hāʻawi i ka lawelawe mea kūʻai aku.Welina mai i kā mākou hui, ua manaʻo mākou e hui pū me ʻoe.
Nā Papa kuhikuhi:

Hāʻawi mau mākou iā ʻoe i kekahi o nā mea kūʻai aku ʻoi loa, a me nā ʻano hoʻolālā ākea a me nā ʻano me nā mea maikaʻi loa.Aia kēia mau hana i ka loaʻa ʻana o nā hoʻolālā maʻamau me ka wikiwiki a me ka hoʻouna ʻana no Fixed Competitive Price Lab Consumables Universal Nucleic Acid Extraction Test Vials Kits, ʻO kā mākou huahana a me nā hoʻonā hauʻoli i ka kaulana kaulana i waena o kā mākou mea kūʻai.Hoʻokipa mākou i nā manaʻo, nā hui hui a me nā hoaaloha pili mai nā wahi āpau o kou honua e launa pū me mākou a ʻimi i ka launa pū ʻana no ka loaʻa ʻana o kekahi.
Kumukūʻai hoʻokūkū paʻaKina Universal Nucleic Acid Extraction a me Nucleic Acid Extraction Extraction, 'O kēlā me kēia mea kū'ai'olu'olu kā mākou pahuhopu.Ke ʻimi nei mākou i ka launa pū ʻana me kēlā me kēia mea kūʻai aku.No ka hoʻokō ʻana i kēia, mālama mākou i kā mākou maikaʻi a hāʻawi i ka lawelawe mea kūʻai aku.Welina mai i kā mākou hui, ua manaʻo mākou e hui pū me ʻoe.

Ke alakaʻi hoʻoponopono pilikia

ʻO kēia ka nānā ʻana o nā pilikia i hiki ke loaʻa i ka unuhi ʻana o ka DNA/RNA viral, me ka manaʻo e kōkua i kāu mau hoʻokolohua.Eia kekahi, no nā pilikia hoʻokolohua a i ʻole nā ​​pilikia ʻenehana ʻē aʻe ma mua o nā ʻōlelo aʻoaʻo a me ka nānā ʻana i nā pilikia, ua hoʻolaʻa mākou i ke kākoʻo ʻenehana e kōkua iā ʻoe.Inā he mau hemahema kāu, e ʻoluʻolu e kelepona mai iā mākou: 028-83360257 a i ʻole leka uila:

Tech@foregene.com.

ʻAʻohe hoʻoheheʻe nucleic acid a i ʻole ka haʻahaʻa haʻahaʻa nucleic acid yield

Nui nā kumu e pili ana i ka hoʻihoʻi ʻana, e like me: sample nucleic acid content, hana hana, elution volume, etc.

Ka nānā 'ana i nā kumu ma'amau:

1. Ua hana ʻia kahi ʻauʻau hau a i ʻole ka wela haʻahaʻa (4 ° C) centrifugation i ka wā o ke kaʻina hana.

Manaʻo: E hana ma ka lumi wela (15-25°C) i ke kaʻina holoʻokoʻa, mai ʻauʻau hau a me ka centrifugation haʻahaʻa haʻahaʻa.

2. Ua mālama pono ʻia ka hāpana a i ʻole ua mālama ʻia ka hāpana no ka lōʻihi loa.

Manaʻo Manaʻo: E mālama i nā laʻana ma -80°C a pale i ka maloʻo a me ka hoʻoheheʻe pinepine ʻana;e ho'āʻo e hoʻohana i nā laʻana i hōʻiliʻili hou ʻia no ka unuhi ʻana i ka waikawa nucleic.

3. lawa ole la'ana lysis.

Manaʻo Manaʻo: E ʻoluʻolu e hui pū ʻia ka hāpana hana a me ka hopena hana lysis a hoʻomoʻa ʻia i ka lumi wela (15-25°C) no 10 mau minuke.

4. Hoʻohui hewa i ka eluent.

Manaʻo Manaʻo: E hōʻoia e hoʻokomo ʻia ka RNase-Free ddH2O i ka hāʻule i ka waena o ka membrane kolamu hoʻomaʻemaʻe, a mai hoʻolei ʻia ma ke apo kolamu hoʻomaʻemaʻe.

5. ʻAʻole i hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer RW2.

Manaʻo Manaʻo: E ʻoluʻolu e hahai i nā ʻōlelo kuhikuhi, e hoʻohui i ka nui kūpono o ka ethanol piha i ka Buffer RW2 a hui maikaʻi ma mua o ka hoʻohana ʻana i ka pahu.

6. Ka nui hāpana kūpono ʻole.

Manaʻo Manaʻo: 200µl o ka laʻana no kēlā me kēia 500µl o Buffer DRL.ʻOi aku ka nui o ka hoʻoheheʻe ʻana i ka laʻana i ka haʻahaʻa haʻahaʻa.

7. Ka nui elution kūpono ʻole a i ʻole ka elution piha ʻole.

Manaʻo: ʻO ka leo eluent o ke kolamu hoʻomaʻemaʻe ʻo 30-50μl;inā ʻaʻole ʻoluʻolu ka hopena elution, pono e hoʻolōʻihi i ka manawa ma ka lumi wela ma hope o ka hoʻohui ʻana i ka RNase-Free ddH2O preheated, e like me 5-10min.

8. Noho ka Ethanol ma ke kolamu ma hope o ka holoi ʻana me Buffer RW2.

Manaʻo Manaʻo: Inā noho ka ethanol ma hope o ka centrifugation me Buffer RW2 no 2 mau minuke, hiki ke waiho ʻia ke kolamu ma ka lumi wela no 5 mau minuke ma hope o ka centrifugation e wehe pono i ke koena ethanol.

Hoʻohaʻahaʻa ʻia ka waikawa nucleic i hoʻomaʻemaʻe ʻia

ʻO ka maikaʻi o ka nucleic acid i hoʻomaʻemaʻe ʻia e pili ana i ka mālama ʻana i ka laʻana, ka hoʻohaumia RNase, ka hana a me nā mea ʻē aʻe.Ka nānā 'ana i nā kumu ma'amau:

1. ʻAʻole mālama ʻia nā laʻana i hōʻiliʻili ʻia i ka manawa.

Manaʻo Manaʻo: Inā ʻaʻole hoʻohana ʻia ka hāpana i ka manawa ma hope o ka hōʻiliʻili ʻana, e ʻoluʻolu e mālama iā ia ma -80°C ma ka wela haʻahaʻa koke.No ka unuhi ʻana i ka RNA, e hoʻohana i nā laʻana i hōʻiliʻili hou ʻia.

2. E hōʻiliʻili i nā laʻana a maloʻo a hoʻoheheʻe pinepine.

Manaʻo Manaʻo: E pale i ka maloʻo a me ka hoʻoheheʻe ʻana (ʻaʻole ʻoi aku ma mua o hoʻokahi manawa) i ka wā o ka hōʻiliʻili ʻana a me ka mālama ʻana i nā laʻana, i ʻole e hoʻemi ʻia ka hua nucleic acid.

3. Hoʻokomo ʻia ʻo RNase i loko o ka lumi hana a ʻaʻole i ʻaʻahu ʻia nā mīkina lima hoʻolele, nā maka, a me nā mea ʻē aʻe.

Manaʻo Manaʻo: Hana maikaʻi ʻia nā hoʻokolohua RNA extraction ma kahi lumi hana RNA kaʻawale, a pono e hoʻomaʻemaʻe ʻia ka papa ʻaina ma mua o ka hoʻokolohua.

E hoʻohana i nā mīkina lima a me nā masks i ka wā o ka hoʻokolohua e pale aku i ka hōʻino ʻana o RNA ma muli o ka hoʻokomo ʻana o RNase i ka nui loa.

4. Ua haumia ka reagent me RNase i ka wā e hoʻohana ai.

Manaʻo: Hoʻololi me kahi Viral DNA/RNA Isolation Kit hou no nā hoʻokolohua pili.

5. Ua haumia me RNase nā paipu centrifuge a me nā ʻōlelo pipette i hoʻohana ʻia no ka hoʻoponopono ʻana i ka RNA.

Manaʻo Manaʻo: E hōʻoia i ka RNase-Free nā paipu centrifuge, nā ʻōlelo pipette, nā pipette, a me nā mea ʻē aʻe i hoʻohana ʻia no ka unuhi ʻana RNA.

Hoʻopili ka waika nucleic hoʻomaʻemaʻe i nā hoʻokolohua ma lalo

Hoʻomaʻemaʻe ʻia ʻo DNA a me RNA e ke kolamu hoʻomaʻemaʻe, inā he kiʻekiʻe ka paʻakai a me ka ʻike protein, e hoʻopilikia ia i nā hoʻokolohua o lalo, e like me: PCR amplification, reverse transcription, etc.

1. He koena paakai ko ka DNA a me RNA i hooheheeia.

Manaʻo Manaʻo: E hōʻoia e hoʻohui ʻia ka leo kūpono o ka ethanol piha i ka Buffer RW2, a holoi i ke kolamu hoʻomaʻemaʻe ʻelua i ka wikiwiki centrifugation i kuhikuhi ʻia ma nā kuhikuhi hana;Hana i ka centrifugation e hōʻemi i ka paʻakai ion contamination.

2. He koena ethanol ka DNA a me ka RNA i hooheheeia.

Manaʻo Manaʻo: Ma hope o ka hōʻoia ʻana i ka holoi ʻana me Buffer RW2, e hana i ka centrifugation paipu ʻole ma ka wikiwiki centrifugation i nā ʻōlelo kuhikuhi;inā he koena ethanol, hiki iā ʻoe ke centrifuge i ka paipu ʻole a waiho i loko o ka lumi wela no 5 mau minuke e wehe i ke koena ethanol i ka nui loa.

Nā Papa kuhikuhi:

ʻO Viral DNA&RNA Isolation Kit Manual