ʻO ka hana hou, ka maikaʻi maikaʻi a me ka hilinaʻi nā kumu waiwai o kā mākou ʻoihana.ʻO kēia mau kumumanaʻo i kēia lā ke kumu o kā mākou kūleʻa ma ke ʻano he hui waena waena o ka honua no ka maikaʻi maikaʻi High Specificity Real Time PCR Kit Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Hoʻolaʻa kā mākou hui i ka hāʻawi ʻana i nā mea kūʻai aku i nā huahana kiʻekiʻe a paʻa i ke kumukūʻai hoʻokūkū, e ʻoluʻolu ai kēlā me kēia mea kūʻai aku i kā mākou huahana a me nā lawelawe.
ʻO ka hana hou, ka maikaʻi maikaʻi a me ka hilinaʻi nā kumu waiwai o kā mākou ʻoihana.ʻO kēia mau loina i kēia lā ke kumu o kā mākou holomua ma ke ʻano he hui waena waena o ka honuaKina Taq DNA Polymerase a me Qpcr, Ke makemake ʻoe i kekahi o kā mākou mau mea ma hope o ka nānā ʻana i kā mākou papa inoa huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no nā nīnau.Hiki iā ʻoe ke hoʻouna mai iā mākou i nā leka uila a hoʻokaʻaʻike mai iā mākou no ke kūkākūkā ʻana a pane mākou iā ʻoe i ka wā e hiki ai.Inā kūpono, hiki iā ʻoe ke ʻike i kā mākou helu wahi ma kā mākou pūnaewele a hele mai i kā mākou ʻoihana.a i ʻole ʻike hou aku o kā mākou mau mea iā ʻoe iho.Mākaukau maʻamau mākou e kūkulu i nā pilina hana lōʻihi a paʻa me nā mea kūʻai aku i loko o nā kahua pili.

Nā wehewehe kime

ʻO ka 2 × Real PCR maʻalahi^TMHāʻawi ʻia ka Mix-SYBR e ka Real Time PCR Easy^TM-SYBR Green I kit he ʻōnaehana premix hou e hoʻohana ana i ka SYBR Green I no ka hoʻonui ʻana i ka PCR manawa maoli, hiki ke hoʻomaikaʻi nui i ka kikoʻī o ka huahana a me ka naʻau pane.Hāʻawi ʻia ʻo ROX ma ke ʻano he kala hoʻomalu kūloko.ʻO ka ikaika o ka fluorescence o kēia pahu he 3-5 mau manawa ma mua o nā huahana like, hiki ke ʻoi aku ka maʻalahi a me ka intuitively e hōʻike i ka ʻike o ka DNA template target.

2×Maʻalahi PCR maoli^TMAia ka Mix-SYBR i ka Taq DNA Polymerase hoʻomaka wela o Foregene.Ke hoʻohālikelike ʻia me nā enzyme Taq maʻamau, loaʻa iā ia nā pōmaikaʻi o ka hoʻonui hoʻonui kiʻekiʻe, ka mana amplification kikoʻī ikaika a me ka haʻahaʻa haʻahaʻa haʻahaʻa.Hiki iā ia ke hōʻemi i ka hoʻonui ʻole kikoʻī a hoʻomaikaʻi i ka pololei o PCR.

Nā kikoʻī

Nā hiʻohiʻona & pono

■ Maʻalahi—2X PCR Mix e hōʻemi i ka hewa hoʻokolohua a me ka manawa hana

■ Hiki ke pale aku i ka hoʻonui ʻole a me ka hoʻokumu ʻana o ka dimer kikoʻī kikoʻī—ʻoi aku ka maikaʻi a me ka hoʻomaka wela Taq enzyme.

■ Kiʻekiʻe sensitivity—hiki ke ʻike i nā kope haʻahaʻa o ka template

■ Maikaʻi maʻalahi—kūpono me ka hapa nui o nā mea kani PCR i ka manawa maoli

Ke noi pahu

ka nānā ʻana qPCR

Ka holo hana

Kiʻi kiʻi

Ka mālama ʻana a me ka mālama ʻana

Pono e mālama ʻia kēia pahu mai ka mālamalama a pono e mālama ʻia ma -20 ℃.Inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4 ℃ no kahi manawa pōkole (10 mau lā).

ʻO ka hana hou, ka maikaʻi maikaʻi a me ka hilinaʻi nā kumu waiwai o kā mākou ʻoihana.ʻO kēia mau loina i kēia lā ke kumu o kā mākou kūleʻa ma ke ʻano he hui waena waena o ka honua no ka maikaʻi maikaʻi High Specificity Real Time PCR Kit Hotstart Taq DNA Polymerase Power Green Qpcr Mix , Hoʻolaʻa kā mākou hui i ka hāʻawi ʻana i nā mea kūʻai aku i nā huahana kiʻekiʻe a paʻa i ke kumukūʻai hoʻokūkū, e ʻoluʻolu i kēlā me kēia mea kūʻai aku i kā mākou huahana a me nā lawelawe.
maikaʻi maikaʻiKina Taq DNA Polymerase a me Qpcr, Ke makemake ʻoe i kekahi o kā mākou mau mea ma hope o ka nānā ʻana i kā mākou papa inoa huahana, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou no nā nīnau.Hiki iā ʻoe ke hoʻouna mai iā mākou i nā leka uila a hoʻokaʻaʻike mai iā mākou no ke kūkākūkā ʻana a pane mākou iā ʻoe i ka wā e hiki ai.Inā kūpono, hiki iā ʻoe ke ʻike i kā mākou helu wahi ma kā mākou pūnaewele a hele mai i kā mākou ʻoihana.a i ʻole ʻike hou aku o kā mākou mau mea iā ʻoe iho.Mākaukau maʻamau mākou e kūkulu i nā pilina hana lōʻihi a paʻa me nā mea kūʻai aku i loko o nā kahua pili.