He ala maikaʻi loa ia e hoʻomaikaʻi ai i kā mākou hāʻina a me ka lawelawe.ʻO kā mākou misionari ka hana ʻana i nā huahana noʻonoʻo i nā mea kūʻai aku me kahi ʻike hana ʻoi aku ka maikaʻi no ka Plasmid DNA Mini Purification Kit maikaʻi, ʻike mākou i ka ʻenehana a me nā mea kūʻai aku ʻo ia ka mea kiʻekiʻe.Hoʻoikaika mākou i ka hana e hana i nā waiwai maikaʻi no kā mākou mea kūʻai aku a hāʻawi i kā mākou mea kūʻai aku i nā mea kūʻai aku a ʻoi aku ka maikaʻi.
He ala maikaʻi loa ia e hoʻomaikaʻi ai i kā mākou hāʻina a me ka lawelawe.ʻO kā mākou ʻoihana e kūkulu i nā huahana inventive i nā mea kūʻai aku me kahi ʻike hana ʻoi aku ka maikaʻi noChina nucleic acid hoʻomaʻemaʻe a me Plasmid DNA Purification, Ke nānā nei mākou i ka hāʻawiʻana i ka lawelawe no kā mākou mau mea kūʻai ma keʻano he mea nui i ka hoʻoikaikaʻana i kā mākou mau pilina lōʻihi.ʻO kā mākou loaʻa mau ʻana o nā mea kūʻai kiʻekiʻe i hui pū ʻia me kā mākou lawelawe mua-kūʻai maikaʻi a ma hope o ke kūʻai aku e hōʻoia i ka hoʻokūkū ikaika i kahi mākeke ulu honua.Makemake mākou e hui pū me nā hoaaloha ʻoihana mai ka home a i waho a hana pū i kahi wā e hiki mai ana.

Nā wehewehe kime

Hiki ke unuhi ʻia a hoʻomaʻemaʻe ʻia nā huahana nucleic acid o FOREGENE mai nā kumu like ʻole a me nā mea hoʻohālike paʻakikī.Hoʻokumu ʻia nā huahana DNA extraction series ma nā kolamu hoʻomaʻemaʻe ʻana o ka membrane silica DNA wale nō a me nā reagents formula kūikawā hiki ke hoʻomaʻemaʻe i ka DNA kiʻekiʻe mai nā ʻano laʻana.

Hoʻohana ka General Plasmid Mini Kit i ka ʻenehana kolamu hoʻomaʻemaʻe DNA-Only a me kahi formula lysis SDS kūpono e loaʻa ai ka DNA plasmid kiʻekiʻe mai ka bacteria i loko o 20 mau minuke.ʻO ka DNA-Only silica gel membrane i hoʻohana ʻia i loko o ke kolamu spin he mea kūʻokoʻa hou ia o FOREGENE, hiki iā ia ke hoʻopili i ka DNA me ka maikaʻi a kikoʻī, a hoʻoneʻe i ka RNA, nā protein haumia, nā ion a me nā mea hoʻohui ʻē aʻe i loko o nā cell i ka nui loa.ʻO ka mana hoʻopaʻa paʻa kiʻekiʻe o ka DNA plasmid o ka kolamu hoʻomaʻemaʻe DNA-Only he 60μg DNA.ʻO ka nui o ka DNA plasmid i unuhi maoliʻia e pili ana i ka nui o ka hopena bacteria, ka helu o nā kope plasmid (kope kiʻekiʻe, kope haʻahaʻa), nā kānana, nā kūlana moʻomeheu a me nā mea'ē aʻe.

Nā kikoʻī

Nā hiʻohiʻona & pono

- ʻAʻohe RNase contamination: ʻO ka DNA-Only Column i hāʻawi ʻia e ka kit e hiki ai ke wehe i ka RNA i loko o ka DNA plasmid me ka hoʻohui ʻole ʻana i ka RNase i ka wā o ka hoʻokolohua, e pale ana i ka hoʻopōʻino ʻia o ka hale hana e ka exogenous RNase.

- Ka wikiwiki wikiwiki: Hiki ke hoʻopau i ka unuhi ʻana o Plasmid DNA i loko o 20 mau minuke.

- Palekana: ʻAʻole koi ʻia ka unuhi ʻana o ka reagent.

- Kiʻekiʻe kiʻekiʻe: ʻO ka plasmid i loaʻa ma ka unuhi ʻana ʻaʻohe RNA, ʻaʻohe RNase a me ka haʻahaʻa haʻahaʻa haʻahaʻa, hiki ke hoʻokō i nā koi o nā hoʻokolohua like ʻole.

Ke noi pahu

ʻO ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA Plasmid no ka maʻi bacteria Gram-negative.

Ka holo hana

Diagram

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia kēia kit no 12 mau mahina ma lalo o nā kūlana maloʻo ma ke ana wela (15-25 ℃);inā pono e mālama ʻia no ka manawa lōʻihi, hiki ke mālama ʻia ma 2-8 ℃.

'Ōlelo Aʻo: Inā mālama ʻia ma kahi wela haʻahaʻa, hiki i ka hāʻina ke hiki i ka ua.Ma mua o ka hoʻohana ʻana, e hōʻoia e waiho i ka hopena i loko o ka pahu i ka lumi wela no kekahi manawa.Inā pono, e hoʻomaʻamaʻa mua iā ia i loko o kahi ʻauʻau wai 37 ℃ no 10 mau minuke e hoʻoheheʻe i ka wai, a hui pū ma mua o ka hoʻohana ʻana.

He ala maikaʻi loa ia e hoʻomaikaʻi ai i kā mākou hāʻina a me ka lawelawe.ʻO kā mākou misionari ka hana ʻana i nā huahana noʻonoʻo i nā mea kūʻai aku me kahi ʻike hana ʻoi aku ka maikaʻi no ka Plasmid DNA Mini Purification Kit maikaʻi, ʻike mākou i ka ʻenehana a me nā mea kūʻai aku ʻo ia ka mea kiʻekiʻe.Hoʻoikaika mākou i ka hana e hana i nā waiwai maikaʻi no kā mākou mea kūʻai aku a hāʻawi i kā mākou mea kūʻai aku i nā mea kūʻai aku a ʻoi aku ka maikaʻi.
ʻAno maikaʻiChina nucleic acid hoʻomaʻemaʻe a me Plasmid DNA Purification, Ke nānā nei mākou i ka hāʻawiʻana i ka lawelawe no kā mākou mau mea kūʻai ma keʻano he mea nui i ka hoʻoikaikaʻana i kā mākou mau pilina lōʻihi.ʻO kā mākou loaʻa mau ʻana o nā mea kūʻai kiʻekiʻe i hui pū ʻia me kā mākou lawelawe mua-kūʻai maikaʻi a ma hope o ke kūʻai aku e hōʻoia i ka hoʻokūkū ikaika i kahi mākeke ulu honua.Makemake mākou e hui pū me nā hoaaloha ʻoihana mai ka home a i waho a hana pū i kahi wā e hiki mai ana.