Loaʻa iā mākou kahi hōʻaiʻē ʻoihana liʻiliʻi maikaʻi, lawelawe ma hope o ke kūʻai aku a me nā hale hana hou, ua loaʻa iā mākou kahi kūlana koʻikoʻi ma waena o kā mākou mea kūʻai aku ma ka honua no High Performance China Convenient Tissue Genomic DNA Nucleic Acid Extraction Purification Reagents, Inā ʻoe e ʻimi nei i kahi kiʻekiʻe, hāʻawi wikiwiki, ʻoi aku ka maikaʻi ma hope o ke kōkua ʻana a me ka mea kūʻai waiwai maikaʻi ma Kina no ka hui lōʻihi o ka hui ʻana.
Loaʻa iā ia kahi hōʻaiʻē ʻoihana liʻiliʻi maikaʻi, lawelawe ma hope o ke kūʻai aku a me nā hale hana hou, ua loaʻa iā mākou kahi kūlana koʻikoʻi ma waena o kā mākou mea kūʻai aku ma ka honua.Mea hoʻomaʻemaʻe ʻakomi nucleic acid, Kino DNA Genomic Tissue Kina, Ua lanakila ko mākou waiwai i ka inoa maikaʻi ma kēlā me kēia o nā lāhui pili.No ka mea, o ka hookumuia ana o ko makou hui.ua koi mākou i kā mākou hana hana hana hou me ke ʻano o ka hoʻokele ʻana o nā lā hou loa, e huki ana i ka nui o nā talena i loko o kēia ʻoihana.Manaʻo mākou i ka hopena maikaʻi loa e like me kā mākou ʻano mea nui loa.

Nā kikoʻī

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.

■ He liʻiliʻi ke koi hāpana, hiki ke hoʻāʻo ʻia e like me ka 5mg ʻiole a i ʻole 2-5mm huelo ʻiole.

■ ʻAʻole koi ʻia nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

■ Ma muli o ka ʻōnaehana PCR maikaʻi loa, ʻoi aku ka maikaʻi o ka PCR a ʻoi aku ka ikaika o ka hoʻomanawanui ʻana i ka PCR reaction inhibitors.

■ Pūnaehana PCR anti-pollution 2×M1-PCR maʻalahi^TMʻO Mix (UNG), ka mea e hoʻopau pono i ka pollution i hoʻokumu ʻia e nā huahana PCR a hōʻoia i ka kikoʻī a me ka pololei o ka amplification.

Nā palena kime

Ka laulā o ka noi: huelo ʻiole, pepeiao ʻiole, ʻiʻo a me nā ʻiʻo ʻē aʻe.

Hoʻokuʻu ʻia ka DNA mai ka laʻana lysis: hoʻohana wale ʻia ma ke ʻano he PCR template.

Hiki ke hoʻohana ʻia ke kit no nā kumu penei: ʻike i ka transgenosis, genotyping holoholona, ​​etc.

Ka holo hana

Ka mālama ʻana a me ka mālama ʻana

Pono e mālama ʻia ka ʻāpana I o kēia pahu ma 2-8 ℃.

Hiki ke mālama ʻia ka reagent Buffer AL no 12 mahina ma lalo o nā kūlana maloʻo;hiki ke mālama ʻia ma 2-8 ℃ no ka mālama lōʻihi.

Loaʻa i ka Reagent Foregene Protease kahi ʻano kūʻokoʻa.No ka hōʻoia ʻana i kāna hana a paʻa, e ʻoluʻolu e mālama iā ia ma 4 ℃ no 12 mahina.

Hiki ke mālama ʻia ka Reagent 6×DNA Loading Buffer ma 4 ℃ a i ʻole -20 ℃ no ka manawa lōʻihi.

Pono e mālama ʻia ka ʻāpana II o kēia pahu ma -20 ℃.

Reagent 2×M1 PCR EasyTM Mix (UNG), inā hoʻohana pinepine ʻia, hiki ke mālama ʻia i ka 4 ℃ no ka mālama manawa pōkole (hoʻohana i loko o 10 mau lā). maikaʻi, hāʻawi wikiwiki, ʻoi aku ka maikaʻi ma hope o ke kōkua a me ka mea kūʻai waiwai maikaʻi ma Kina no ka pilina hui lōʻihi, e lilo mākou i kāu koho maikaʻi loa.
Hana KiʻekiʻeKino DNA Genomic Tissue Kina, Mea hoʻomaʻemaʻe ʻakomi nucleic acid, Ua lanakila ko mākou waiwai i ka inoa maikaʻi ma kēlā me kēia o nā lāhui pili.No ka mea, o ka hookumuia ana o ko makou hui.ua koi mākou i kā mākou hana hana hana hou me ke ʻano o ka hoʻokele ʻana o nā lā hou loa, e huki ana i ka nui o nā talena i loko o kēia ʻoihana.Manaʻo mākou i ka hopena maikaʻi loa e like me kā mākou ʻano mea nui loa.