ʻO ke kūlana kiʻekiʻe kiʻekiʻe a hilinaʻi maikaʻi ʻo kā mākou mau loina, e kōkua iā mākou ma kahi kūlana kiʻekiʻe.Ke hoʻomau nei i kāu kumu o ka "maikaʻi loa, ka mea kūʻai kiʻekiʻe" no ka mea kūʻai aku ma ka pūnaewele Viru Extraction Magnetic Beads Method Viral DNA / Rna Nucleic Acid Extraction Reagent Kits no Real Time PCR Detection me CE, ʻOiai e hoʻohana ana i ka hoʻomaikaʻi ʻana o ke kaiāulu a me ka hoʻokele waiwai, e paʻa kā mākou ʻoihana i kahi ʻōlelo o "E nānā i ka hilinaʻi, kiʻekiʻe kiʻekiʻe ka mea mua".
ʻO ke kūlana kiʻekiʻe kiʻekiʻe a hilinaʻi maikaʻi ʻo kā mākou mau loina, e kōkua iā mākou ma kahi kūlana kiʻekiʻe.ʻO ka hoʻopili ʻana i kāu kumu o "ka maikaʻi loa, ka mea kūʻai kiʻekiʻe" noʻO ka DNA koko Kina a me ka pahu hoʻoheheʻe, Ke koi nei kā mākou hui i ke kumu o ka "lawe i ka lawelawe ma mua no ka maʻamau, hōʻoia maikaʻi no ka brand, hana i ka ʻoihana me ka manaʻo maikaʻi, e hoʻolako i ka lawelawe kūpono, wikiwiki, pololei a me ka manawa kūpono no ʻoe".Hoʻokipa mākou i nā mea kūʻai kahiko a me nā mea hou e kūkākūkā me mākou.E lawelawe mākou iā ʻoe me ka ʻoiaʻiʻo!

Nā kikoʻī

200 ×20μl rxns, 2000 ×20μl rxns 2× SuperEasy^TMMix-EDTA: Loaʻa i ka D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc.₂O i ka 2× SuperEasy^TMHoʻohui-EDTA e hoʻohana ʻia i ka hopena PCR.

6× DNA Loading Buffer: ʻAʻohe SDS i ka Loading Buffer.Manaʻo ʻia e hoʻohana i ka 6 × DNA Loading Buffer i hoʻokomo ʻia me ka pahu i ka wā e hana ai i ka electrophoresis gel agarose i mea e loaʻa ai nā hopena electrophoresis maikaʻi.Mai hoʻohana i ka Loading Buffer i loaʻa iā SDS, i ʻole e loaʻa kahi huelo nui o ke kukui ʻālohilohi i ke ala i ka wā electrophoresis, e pili ana i nā hopena hoʻokolohua.

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

-ʻAʻohe pono no ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi, ʻaʻohe pretreatment, a me ka hoʻohana pololei ʻana i ke koko holoʻokoʻa ma ke ʻano he PCR template.-He mana hoʻonui ikaika ka ʻōnaehana a hiki ke ʻike maʻalahi i ka genome a me nā ʻāpana DNA target haole i loko o ke koko.-2× Māmā^TMMix-EDTA Ka hoʻomanawanui koko kiʻekiʻe: a hiki i ka 45% i ke koko kanaka a me 20% i ke koko ʻiole.- Hoʻopili piha ʻia ka laʻana, no laila ʻaʻohe pono e hopohopo e pili ana i ka hoʻohaumia ʻana o ka laʻana a me nā hopena PCR maikaʻi ʻole.

Ke noi pahu

Hoʻolaʻa ʻia i ka ʻike PCR pololei o ke koko holoʻokoʻa anticoagulated EDTA.(E like me: koko kanaka, koko ʻiole, koko moa, koko manu, koko bipi, koko ʻīlio, etc.)

Kaʻina hana

Ka mālama ʻana a me ka mālama ʻana

Pono e mālama ʻia kēia pahu ma kahi -20 ° C.2× Māmā^TMHoʻohui-EDTA, inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4 ℃ no ka mālama pōkole (hoʻohana i loko o 10 mau lā).6 × DNA Loading Buffer，Hiki ​​ke mālama ʻia ma 4 ° C a i ʻole -20 ° C no ka manawa lōʻihi. ʻO ka hilinaʻi kiʻekiʻe kiʻekiʻe a me ka maikaʻi o ka hōʻaiʻē kūlana ko mākou mau kumu, e kōkua iā mākou ma kahi kūlana kiʻekiʻe.Ke hoʻomau nei i kāu kumu o ka "maikaʻi loa, ka mea kūʻai kiʻekiʻe" no ka mea kūʻai aku ma ka pūnaewele Viru Extraction Magnetic Beads Method Viral DNA / Rna Nucleic Acid Extraction Reagent Kits no Real Time PCR Detection me CE, ʻOiai e hoʻohana ana i ka hoʻomaikaʻi ʻana o ke kaiāulu a me ka hoʻokele waiwai, e paʻa kā mākou ʻoihana i kahi ʻōlelo o "E nānā i ka hilinaʻi, kiʻekiʻe kiʻekiʻe ka mea mua".
Mea hoʻopuka pūnaeweleʻO ka DNA koko Kina a me ka pahu hoʻoheheʻe, Ke koi nei kā mākou hui i ke kumu o ka "lawe i ka lawelawe ma mua no ka maʻamau, hōʻoia maikaʻi no ka brand, hana i ka ʻoihana me ka manaʻo maikaʻi, e hoʻolako i ka lawelawe kūpono, wikiwiki, pololei a me ka manawa kūpono no ʻoe".Hoʻokipa mākou i nā mea kūʻai kahiko a me nā mea hou e kūkākūkā me mākou.E lawelawe mākou iā ʻoe me ka ʻoiaʻiʻo!