Pono kā mākou komisina e lawelawe i kā mākou mea hoʻohana hope a me nā mea kūʻai aku me nā huahana kiʻi kiʻi kiʻi kiʻi maikaʻi loa a me nā hoʻonā noPCR Reagent, Hoʻokipa maikaʻi mākou i nā hui ʻoihana ʻoihana ʻelua ma waho a me ka home, a manaʻolana mākou e hana pū me ʻoe i ka wā lōʻihi!
Pono kā mākou komisina e lawelawe i kā mākou mea hoʻohana hope a me nā mea kūʻai aku me nā huahana kiʻi kiʻi kiʻi kiʻi maikaʻi loa a me nā hoʻonā noPCR Reagent, Me kahi kokoke i 30 mau makahiki i ka ʻoihana, ua hilinaʻi mākou i ka lawelawe ʻoi aku ka maikaʻi, ka maikaʻi a me ka lawe ʻana.Hoʻokipa maikaʻi mākou i nā mea kūʻai mai ka honua holoʻokoʻa e hui pū me kā mākou hui no ka hoʻomohala maʻamau.

Nā kikoʻī

5ml, 10ml, 40ml

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ ʻOi aku ka hilinaʻi: 6 manawa i ko Taq enzyme maʻamau;

■ ʻOi aku ka wikiwiki o ka hoʻonui ʻana : ʻo 5-10sec/kb ka māmā hoʻonui, ʻo ia ka 6-12 mau manawa i ko Taq enzyme maʻamau;

■ Hiki ke hoʻololi hou aku i ka laʻana: Hiki iā ia ke hoʻonui i nā ʻano maʻamau DNA paʻakikī me ke kiʻekiʻeGC maʻiʻo;

■ ʻOi aku ka maikaʻi o ka hoʻonui ʻana: nā pōʻai hoʻonui haʻahaʻa ma mua o ka enzyme Taq maʻamau;

■ ʻOi aku ka ikaika o ka hoʻomanawanui ʻana o ke kaiapuni: kau ʻia ma 37°C no hoʻokahi pule, mālama ʻia ma mua o 90% hana;

■ He 5′ →3′ DNA polymerase hana a me 5′→3′ exonuclease hana, me ka 3′ →5′ hana exonuclease.

■ Ua hui mua ka pahu me ka Electrophoresis Loading Buffer, ma hope o ka pau ʻana o ka PCR, hiki ke hoʻouka pololei ʻia ka hāpana no ka electrophoresis;maʻalahi a maʻalahi ka hana.

Ke noi pahu

Hoʻonui ʻia ka PCR o nā ʻāpana DNA, ka hōʻailona DNA, ka hoʻopili ʻana i ka DNA, PCR me kahi huelo

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia no ka manawa lōʻihi ma -20 ° C;no ka hoʻohana pinepine, hiki ke mālama ʻia ma 4 ° C no ka manawa pōkole. ʻO ka 2 × Real PCR Easy TM Mix-SYBR i hāʻawi ʻia e ka Real Time PCR EasyTM-SYBR Green I kit he ʻōnaehana premix hou e hoʻohana ana i ka SYBR Green I no ka Real Time PCR amplification reactions, hiki ke hoʻomaikaʻi nui i ka kikoʻī o ka huahana a me ka naʻau pane.Hāʻawi ʻia ʻo ROX ma ke ʻano he kala hoʻomalu kūloko.ʻO ka ikaika o ka fluorescence o kēia pahu he 3-5 mau manawa ma mua o nā huahana like, hiki ke ʻoi aku ka maʻalahi a me ka intuitively e hōʻike i ka ʻike o ka DNA template target.
2×Real PCR EasyTM Mix-SYBR loaʻa iā Foregene kahi wela hoʻomaka Taq DNA Polymerase.Ke hoʻohālikelike ʻia me nā enzyme Taq maʻamau, loaʻa iā ia nā pōmaikaʻi o ka hoʻonui hoʻonui kiʻekiʻe, ka mana amplification kikoʻī ikaika a me ka haʻahaʻa haʻahaʻa haʻahaʻa.Hiki iā ia ke hōʻemi i ka hoʻonui ʻole kikoʻī a hoʻomaikaʻi i ka pololei o PCR.