ʻO kā mākou holomua e hilinaʻi nei i loko o nā huahana kiʻekiʻe, nā kālena maikaʻi a me nā ikaika ʻenehana i hoʻoikaika pinepine ʻia no ka Quality Inspection for China Utm Virus Kits DNA Test Sampling Tube Oral Swabs Viral Transport Medium Tubes Nasal Swab Vtm Ki, Inā ʻoe e ʻimi nei i kahi kiʻekiʻe kiʻekiʻe, hāʻawi wikiwiki, ʻoi aku ka maikaʻi ma hope o ke kākoʻo a me ka mea kūʻai waiwai nui ma Kina no ka pilina ʻoihana liʻiliʻi lōʻihi, e lilo mākou i koho maikaʻi loa.
Aia kā mākou holomua i loko o nā huahana holomua, nā kālena maikaʻi a me nā pūʻali ʻenehana i hoʻoikaika pinepine ʻia noKina Vtm Tube, Haahaa Vtm 10 Ml Tube, Ma ke ʻano he hale hana akamai mākou e ʻae pū i ke kauoha maʻamau a hana like me kāu kiʻi a i ʻole ka laʻana e hōʻike ana i ka kikoʻī a me ka hoʻolālā ʻana o ka mea kūʻai aku.ʻO ka pahuhopu nui o ka hui ʻo ia ke ola i kahi hoʻomanaʻo maikaʻi i nā mea kūʻai aku a pau, a hoʻokumu i kahi pilina ʻoihana lanakila-lanakila lōʻihi.No ka ʻike hou aku, e hoʻomanaʻo e hoʻokaʻaʻike mai iā mākou.A ʻo ko mākou hauʻoli nui inā makemake ʻoe e hālāwai pilikino ma kā mākou keʻena.

wehewehe

50 Mākaukau, 200 Mākaukau

Ke hoʻohana nei kēia pahu i ke kolamu spin a me ke ʻano i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA piha maʻemaʻe kiʻekiʻe a kiʻekiʻe mai nā cell i hoʻoulu ʻia ma nā papa 96, 24, 12, a me 6-well.

Hāʻawi ka pahu i kahi kolamu hoʻomaʻemaʻe DNA maikaʻi, hiki ke hoʻokaʻawale i ka supernatant a me ka cell lysate, hoʻopaʻa a wehe i ka DNA genomic.He maʻalahi ka hana a mālama manawa.

Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA me kahi ʻano kū hoʻokahi.Hiki ke hana ʻia ka nui o nā laʻana i ka manawa like.

Nā ʻāpana pahu

*E ʻoluʻolu e ʻaʻahu i nā mīkina lima a e hana i nā hana pale i ka wā o ka hana ʻana no ka mea aia ʻo Buffer cRL1 a me Buffer RW1 i nā paʻakai chaotropic hoʻonāukiuki.

Nā hiʻohiʻona & pono

■ Hoʻohana ʻia ke kaʻina holoʻokoʻa ma ka lumi wela (15-25 ℃), me ka ʻauʻau hau a me ka centrifugation haʻahaʻa.
■ ʻO ka pahu holoʻokoʻa he RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopaʻa pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki i ka pahu ke hoʻopau i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase hou.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi ka hana a hiki ke hoʻopau i nā minuke 11.
■ Palekana: ʻAʻole koi ʻia ka mea hoʻoponopono organik.
■ Ke kūlana kiʻekiʻe: ʻO ka RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka nui o ka RNA mai nā cell moʻomeheu i nā papa 96, 24, 12, a me 6-well.

Ka holo hana

Diagram

Ua mālama ʻia ke kiʻi pākaukau agarose gel o ka Cell Total RNA Isolation Kit i nā helu like ʻole o luna, 20μl volume elution, e lawe i ka 2μl i hoʻomaʻemaʻe ʻia i ka RNA 1%.

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ʻo Buffer cRL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho). ʻO kā mākou holomua e pili ana i loko o nā huahana kiʻekiʻe, nā talena fantastic a me ka hoʻoikaika pinepine ʻana i nā pūʻali ʻenehana no ka Quality Inspection for China Utm Virus Kits DNA Test Sampling Tube Oral Swabs Viral Transport Medium Tubes Nasal Swab ke kākoʻo maikaʻi loa ma hope o ka hāʻawi ʻana i ka waiwai maikaʻi loa. ma Kina no ka pilina ʻoihana liʻiliʻi lōʻihi, ʻo mākou kāu koho maikaʻi loa.
Nānā maikaʻi noKina Vtm Tube, Haahaa Vtm 10 Ml Tube, Ma ke ʻano he hale hana akamai mākou e ʻae pū i ke kauoha maʻamau a hana like me kāu kiʻi a i ʻole ka laʻana e hōʻike ana i ka kikoʻī a me ka hoʻolālā ʻana o ka mea kūʻai aku.ʻO ka pahuhopu nui o ka hui ʻo ia ke ola i kahi hoʻomanaʻo maikaʻi i nā mea kūʻai aku a pau, a hoʻokumu i kahi pilina ʻoihana lanakila-lanakila lōʻihi.No ka ʻike hou aku, e hoʻomanaʻo e hoʻokaʻaʻike mai iā mākou.A ʻo ko mākou hauʻoli nui inā makemake ʻoe e hālāwai pilikino ma kā mākou keʻena.