Moʻo pololei RT-qPCR
PCR pololei
He aha ka Direct PCR
ʻO Direct PCR ke ʻano o ka hoʻonui pololei ʻana i ka DNA mai ke koko, ka ʻiʻo holoholona, ka huelo ʻiole, ke kelepona, ka pepeiao ʻiole, ka iʻa zebra, nā hua iʻa, nā lau mea kanu, nā ʻanoʻano a i ʻole nā mea hoʻohālike o ke kino ola kino me ka hana ʻole i ka hoʻokaʻawale DNA a me nā hana hoʻomaʻemaʻe.Hiki i ka ʻenehana PCR pololei ke hōʻemi nui i ka manawa hoʻokolohua, a me ke kumu kūʻai i ka genotyping a me nā papahana kiʻekiʻe.Hāʻawi ia i kahi koho ʻoi aku ka maikaʻi i ka wā e kū ai i nā pilikia o ka hoʻonui ʻana i kahi laʻana liʻiliʻi loa kahi e hiki ai i ka hana hoʻomaʻemaʻe ke alakaʻi i ka nalowale o ka laʻana.
He koʻikoʻi koʻikoʻi ko FOREGENE's PCR Mix, no ka ikaika o FOREGENE i hoʻopaʻa ʻia ʻo Taq enzyme (ʻae ʻia: ZL20161034512.0 (Kina), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Iapana)).Hoʻohana kāna Taq enzyme i ka ʻenehana hoʻohui DNA e hoʻohui hou i ka ʻāpana hoʻopaʻa paʻa DNA o ka archaeal nucleic acid binding protein me ka Taq DNA polymerase wela e kūkulu i kahi DNA template me ka hoʻonui ʻana i ka pilina.ʻO kahi Taq hoʻomaka wela i hoʻomaikaʻi ʻia e mālama ana i nā hana like ʻole o ka DNA polymerase mua. Hiki i kēia Taq enzyme ke hoʻopaʻa i ka DNA template a hoʻomaka i ka synthesis DNA i nā kumu hoʻohālikelike haʻahaʻa a me nā kaiapuni paʻakikī.
No ka laʻana, maʻalahi ia e like me ka hoʻohui ʻana i kāu kelepona, mea kanu (lau ʻōpio, aʻa a hua paha) a i ʻole ka laʻana holoholona i ka buffer, a me ka hoʻohui ʻana i ka master mix me nā primers kikoʻī i loko o nā paipu PCR a me ka holo ʻana ma o ka mea hoʻokele wela.He mea maikaʻi ka hana kit no ka nānā ʻana i ka laʻana nui kahi mea nui e mālama ai i ka manawa.
Ua koho ʻia ka ʻōnaehana hopena PCR e loaʻa ana i ka enzyme UNG a me dUTP e like me nā koi hoʻokolohua, hiki ke pale pono i ka pollution i hoʻokumu ʻia e nā huahana amplification PCR.
Pono oDPCR pololei
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| PCR pololei | ʻAno kuʻuna (Hoʻomaʻemaʻe RNA + PCR Kuʻuna) |
Hoʻohana waiwai | Hoʻohana ʻiʻo | Liʻiliʻi | Nui |
Hoʻomākaukau template | ʻAno mea | ʻAʻole pono ka wili | Nā laʻana wili |
Hana | Hoʻokahi ʻano paipu, 10 min | hana paʻakikī, 1h | |
ʻO ka lele | 1-96 | 1-24 | |
ka pane PCR | ʻōnaehana pane | Hoʻopili ʻia ʻo 2×PCR hui | Dntp, MgCl2,10×PCR Buffer, Taq enzyme, |
Application
01 Ka ʻike ʻana i nā microorganism pathogenic
02 Hōʻikeʻike i hoʻololi ʻia, genotyping
03 Multiplex PCR / SNP ʻike / PCR-RFLP
04Ka hoʻokaʻina ʻana/cloning
Huahana Huahana
moʻo | inoa mea kūʻai | Nā kikoʻī | Helu Helu | Nā Kūlana Waihona |
Kuhikuhi RT-qPCR Series | 200T | DRT-01011 | Mahele I 4 ℃, Māhele II -20 ℃ | |
1000T | DRT-01012 |
moʻo | inoa mea kūʻai | Nā kikoʻī | Helu Helu | Nā Kūlana Waihona |
Koko Direct PCR Series | 200 ×20μl rxns | TP-04111 | -20 ℃ | |
2000 ×20μl rxns | TP-04113 | |||
200 ×20μl rxns | TP-04121 | -20 ℃ | ||
2000 ×20μl rxns | TP-04123 | |||
200 ×20μl rxns | TP-04211 | -20 ℃ | ||
2000 ×20μl rxns | TP-04213 | |||
200 ×20μl rxns | TP-04221 | -20 ℃ | ||
2000 ×20μl rxns | TP-04223 |
Series | inoa mea kūʻai | Nā kikoʻī | Helu Helu | Nā Kūlana Waihona |
Holoi PCR Holoi holoholona | Kiko PCR pololei o nā kino holoholona (65 ℃ lysis) | 200 ×20μl rxns | TP-01111 | Mahele I 4 ℃, Māhele II -20 ℃ |
2000 ×20μl rxns | TP-01113 | |||
ʻO ka pahu PCR pololei o ke kino holoholona (65 ℃ lysis) -UNG | 200 ×20μl rxns | TP-01121 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-01123 | |||
Huelo iole SuperDirectTMKiko PCR (lysis wela lumi) | 200 ×20μl rxns | TP-01311 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-01313 | |||
Kit PCR pololei huelo iole (65 ℃ lysis) | 200 ×20μl rxns | TP-01331 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-01333 | |||
Kiko PCR pololei huelo iole (65 ℃ lysis) - UNG | 200 ×20μl rxns | TP-01341 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-01343 | |||
ʻO Zebra Fish Direct PCR Kit(65℃ lysis) | 200 ×20μl rxns | TP-01411 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-01413 | |||
Zebra Fish Direct PCR Kit(65℃ lysis) - UNG | 200 ×20μl rxns | TP-01421 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-01423 |
moʻo | inoa mea kūʻai | Nā kikoʻī | Helu Helu | Nā Kūlana Waihona |
Mea kanu Direct PCR Series | 200 ×20μl rxns | TP-02111 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-02113 | |||
200 ×20μl rxns | TP-02121 | Mahele I 4 ℃, Māhele II -20 ℃ | ||
2000 ×20μl rxns | TP-02123 | |||
Mea kanu Leaf Direct PCR Plus Kit | 200 ×20μl rxns | TP-02131 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-02133 | |||
Mea kanu Leaf Direct PCR Plus Kit -UNG | 200 ×20μl rxns | TP-02141 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-02143 | |||
200 ×20μl rxns | TP-03111 | Mahele I 4 ℃, Māhele II -20 ℃ | ||
2000 ×20μl rxns | TP-03113 | |||
200 ×20μl rxns | TP-03121 | Mahele I 4 ℃, Māhele II -20 ℃ | ||
2000 ×20μl rxns | TP-03123 | |||
200 ×20μl rxns | TP-03131 | Mahele I 4 ℃, Māhele II -20 ℃ | ||
2000 ×20μl rxns | TP-03133 | |||
200 ×20μl rxns | TP-03141 | Mahele I 4 ℃, Māhele II -20 ℃ | ||
2000 ×20μl rxns | TP-03143 | |||
Mea kanu ʻO Direct PCR Plus Kit I(Polysaccharide polyphenol plant) | 200 ×20μl rxns | TP-03151 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-03153 | |||
Mea kanu ʻO Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) | 200 ×20μl rxns | TP-03161 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-03163 | |||
200 ×20μl rxns | TP-03171 | Mahele I 4 ℃, Māhele II -20 ℃ | ||
2000 ×20μl rxns | TP-03173 | |||
Nā Hua kanu Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) | 200 ×20μl rxns | TP-03181 | Mahele I 4 ℃, Māhele II -20 ℃ | |
2000 ×20μl rxns | TP-03183 |