ʻO China Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Mouse Tail) (no Genotyping) Mea hana a mea hoʻolako |Foregene

ʻO ka ʻiole Kiʻi PCR pololei ʻo Direct PCR Lysis Reagent(Mouse Tail)(no ka Genotyping)

Kiʻi PCR Kiʻi Kiʻi ʻIole Direct PCR Lysis Reagent(Huo Iole)(no ka Genotyping) Kiʻi Hōʻikeʻike

ʻO ka wehewehe ʻana o ka pahu:

E hoʻohana pololei i ka lysate huelo ʻiole ma ke ʻano he kumu hoʻohālike no ka hoʻonui ʻana i ka PCR, me ka ʻole o ka unuhi ʻokoʻa o ka DNA, wikiwiki a kiʻekiʻe.

◮ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.

◮He liʻiliʻi ke koi hāpana, lawe wale i hoʻokahi hua.

◮ʻAʻole koi ʻia nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

◮ʻO ka ʻōnaehana PCR i hoʻopaʻa ʻia e hiki ai i ka PCR ke loaʻa ka kikoʻī kiʻekiʻe aʻe a ʻoi aku ka ikaika o ka hoʻomanawanui i ka PCR reaction inhibitors.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Nā wehewehe

Ke hoʻohana nei kēia pahu i kahi ʻōnaehana lysis buffer kūʻokoʻa e hoʻokuʻu koke i ka DNA genomic mai nā huelo ʻiole, nā pepeiao, nā ʻiʻo a me nā ʻano kikoʻī ʻē aʻe no ka hopena PCR, no laila ua kūpono loa ia no ka hoʻāʻo ʻana o ka genetic nui.

Hoʻopau ʻia ke kaʻina hana o ka hoʻokuʻu ʻana i ka DNA genomic mai ka lysis buffer i loko o 10-30 mau minuke ma 65 ° C.ʻAʻole koi ʻia nā kaʻina hana ʻē aʻe e like me ka protein a me ka wehe ʻana i ka RNA, a hiki ke hoʻohana ʻia ka trace DNA i hoʻokuʻu ʻia ma ke ʻano he kumu hoʻohālike no ka hopena PCR.

2× M1-PCR maʻalahi^TMHiki i ka Mix ke hoʻomanawanui ikaika i ka PCR reaction inhibitors, a hiki ke hoʻohana i ka lysate o ka hāpana e hoʻāʻo ʻia ma ke ʻano he laʻana no ka hoʻonui pono a kikoʻī.Aia kēia reagent i ka Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, pale pale, PCR optimizer a stabilizer.

ʻO D-Taq DNA polymerase kahi DNA polymerase i hoʻomohala kūikawā ʻia e Foregene no nā hopena PCR pololei.Hiki i ka D-Taq DNA polymerase ke hoʻomanawanui i ka PCR reaction inhibitors, a hiki ke hoʻonui i ka nui o ka DNA i loko o nā ʻōnaehana hoʻololi paʻakikī, a hiki i ka wikiwiki hoʻonui ke hiki i 2Kb/min, kūpono loa ia no ka pane pololei PCR.

Nā kikoʻī

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Nā ʻāpana pahu

Mahele I	Palena MP
	Foregene Protease Plus
	6× DNA Hoʻouka Pahu
Mahele II	2× M1 PCR maʻalahi^TMHuikau
Nā kuhikuhi

Nā hiʻohiʻona & pono

■ʻAʻole pono ka hoʻomaʻemaʻe DNA hoʻopau manawa a me ka pipiʻi.

■ He liʻiliʻi ke koi hāpana, hiki ke hoʻāʻo ʻia e like me ka 5mg ʻiole a i ʻole 2-5mm huelo ʻiole.

■ʻAʻole pono nā hana kūikawā e like me ka wili a me ka ʻoki ʻana, a maʻalahi ka hana.

■ Ma muli o ka ʻōnaehana PCR maikaʻi loa, ʻoi aku ka maikaʻi o ka PCR a ʻoi aku ka ikaika o ka hoʻomanawanui ʻana i ka PCR reaction inhibitors.

Nā palena kime

Hoʻohana: Hulo ʻiole, pepeiao ʻiole, ʻiʻo a me nā ʻiʻo ʻē aʻe.

Hoʻokuʻu ʻia ka DNA mai ka laʻana lysis: hoʻohana wale ʻia ma ke ʻano he PCR template.

Hiki ke hoʻohana ʻia ke kit no nā kumu penei: ʻike i ka transgenosis, genotyping holoholona, etc.

Ka holo hana

Ka mālama ʻana a me ka mālama ʻana

Pono e mālama ʻia ka ʻāpana I o kēia pahu ma 2-8℃.

Hiki ke mālama ʻia ka reagent Buffer AL no 12 mahina ma lalo o nā kūlana maloʻo;hiki ke mālama ʻia ma 2-8℃no ka waiho loihi ana.

Loaʻa i ka Reagent Foregene Protease kahi ʻano kūʻokoʻa.No ka hōʻoia ʻana i kāna hana a me ka paʻa, e mālama iā ia ma 4℃no 12 mahina.

6×Hiki ke mālama ʻia ka DNA Loading Buffer ma 4℃aiʻole -20℃no ka manawa loihi.

Pono e mālama ʻia ka ʻāpana II o kēia pahu ma -20℃.

Mea hoʻoponopono 2×M1 PCR maʻalahi^TMHoʻohui, inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4℃no ka mālama manawa pōkole (hoʻohana i loko o 10 mau lā).

Mua: Kiko PCR pololei huelo iole-UNG Direct PCR Lysis Reagent(Huo Iole)(no ka Genotyping)

Aʻe: Kit PCR Tissue Direct Animal (me ka ʻole o nā mea hana laʻana).

E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou