Ua ʻike mākou i ka mea hana.Ke lanakila nei i ka hapa nui o kāu mau palapala hōʻoia koʻikoʻi o kāna mākeke no Super Purchasing for China Real-Time PCR, Inā makemake ʻoe i kahi kokoke i nā waiwai, e hoʻomanaʻo e ʻoluʻolu maoli e launa pū me mākou no nā ʻike hou aʻe a i ʻole e hāʻawi pololei iā mākou i ka leka uila, e pane mākou iā ʻoe i loko o 24 mau hola a me ka ʻōlelo maikaʻi loa e hāʻawi ʻia.
Ua ʻike mākou i ka mea hana.Ke lanakila nei i ka hapa nui o kāu mau palapala hōʻoia koʻikoʻi o kāna mākeke noKina PCR, Pcr, Me ka manaʻo hoʻoikaika o ka "kiʻekiʻe kiʻekiʻe, ʻoluʻolu, kūpono a me ka hana hou", a e like me ke alakaʻi lawelawe ʻana o ka "maikaʻi maikaʻi akā ʻoi aku ka maikaʻi o ke kumukūʻai," a me ka "aie honua", ke hoʻoikaika nei mākou e hui pū me nā hui ʻāpana kaʻa ma ka honua holoʻokoʻa e hana i kahi hui lanakila-lanakila.

Nā wehewehe

Hoʻohana kēia kit i kahi ʻōnaehana lysis buffer kūʻokoʻa e hiki ke hoʻokuʻu koke i ka RNA mai nā laʻana cell moʻomeheu no nā hopena RT-qPCR, a laila e hoʻopau i ke kaʻina hoʻomaʻemaʻe RNA hoʻopau manawa a paʻakikī.Hiki ke loaʻa ka template RNA i loko o 7 mau minuke.Hiki i ka 5×Direct RT Mix a me ka 2×Direct qPCR Mix-SYBR reagents i hāʻawi ʻia e ka kit hiki ke loaʻa koke i nā hopena PCR quantitative maoli.

ʻO 5 × Direct RT Mix a me 2 × Direct qPCR Mix-SYBR he ikaika ka hoʻomanawanui inhibitor, a hiki ke hoʻohana ʻia ka lysate o nā laʻana e like me ke kumu hoʻohālike no RT-qPCR pololei.Aia kēia pahu i ka RNA high-affinity Foregene reverse transcriptase, a me Hot D-Taq DNA polymerase, dNTPs, MgCl₂, pale pale, PCR optimizer a stabilizer.

Nā kikoʻī

200×20μl Rxns, 1000×20μl Rxns

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ Maʻalahi a maikaʻi : me ka ʻenehana Cell Direct RT, hiki ke loaʻa nā laʻana RNA i loko o 7 mau minuke.

■ He liʻiliʻi ka noi hāpana, hiki ke hoʻāʻo ʻia ma kahi haʻahaʻa he 10.

■ Kiʻekiʻe throughput: hiki iā ia ke ʻike koke i ka RNA i loko o nā cell moʻomeheu i 384, 96, 24, 12, 6-well plates.

■ Hiki i ka DNA Eraser ke wehe koke i nā genome i hoʻokuʻu ʻia, e hoʻemi nui i ka hopena ma nā hopena hoʻokolohua ma hope.

■ ʻOi aku ka maikaʻi o ka ʻōnaehana RT-PCR i ʻoi aku ka maikaʻi a me ka PCR i ʻoi aku ka maikaʻi, a ʻoi aku ke kūpaʻa ʻana i nā mea hoʻopaneʻe hopena RT-qPCR.

Ke noi pahu

Ka laulā o ka hoʻohana ʻana: nā cell moʻomeheu.

- Hoʻokuʻu ʻia ʻo RNA e ka laʻana lysis: pili wale i ka template RT-qPCR o kēia pahu.

- Hiki ke hoʻohana ʻia ke kit no nā kumu aʻe: ka nānā ʻana i ka hōʻike ʻana o ke ʻano, ka hōʻoia o ka hopena silencing gene-mediated gene siRNA, ka nānā ʻana i ka lāʻau, etc.

Diagram

Ka mālama ʻana a me ka mālama ʻana

Pono e mālama ʻia ka ʻāpana I o kēia pahu ma 4 ℃;Pono e mālama ʻia ka ʻāpana II ma -20 ℃.

Pono e mālama ʻia ka Foregene Protease Plus II ma 4 ℃, mai maloʻo ma -20 ℃.

Pono e mālama ʻia ka Reagent 2×Direct qPCR Mix-SYBR ma -20 ℃ i ka pōʻeleʻele;inā hoʻohana pinepine ʻia, hiki ke mālama ʻia ma 4 ℃ no ka mālama manawa pōkole (hoʻohana i loko o 10 mau lā). Ua ʻike mākou i ka mea hana.ʻO ka lanakila ʻana i ka hapa nui o kāu mau palapala hōʻoia koʻikoʻi o kāna mākeke no Super Purchasing no Kina Q160/Q320 Real-Time PCR, Inā makemake ʻoe i nā waiwai āpau, e hoʻomanaʻo e ʻoluʻolu maoli e launa pū me mākou no nā ʻike hou aʻe a i ʻole e hāʻawi pololei iā mākou i ka leka uila, e pane mākou iā ʻoe i loko o 24 mau hola a me ka ʻōlelo maikaʻi loa e hāʻawi ʻia.
Kūʻai Nui noKina PCR, Real Time PCR, Me ka manaʻo hoʻoikaika o ka "kiʻekiʻe o ka pono, ka maʻalahi, ka hana a me ka hana hou", a e like me ke alakaʻi lawelawe ʻana o "maikaʻi maikaʻi akā ʻoi aku ka maikaʻi o ke kumukūʻai," a me ka "aie honua", ke hoʻoikaika nei mākou e hui pū me nā ʻāpana kaʻa kaʻa a puni ka honua e hana i kahi hui lanakila-lanakila.