Hoʻopili ʻo ia i ka ʻōlelo "Hoʻopono, hana, ʻoihana, ʻano hou" e hoʻomohala mau i nā huahana hou.Manaʻo ʻo ia i nā mea kūʻai aku, kūleʻa e like me kona kūleʻa ponoʻī.E hoʻomohala mākou i ka lima maikaʻi o ka wā e hiki mai ana no Wholesale spin column Method Nucleic Acid Extraction Reagent Bottle Kits, Hiki iā mākou ke hāʻawi iā ʻoe i kekahi o nā kumukūʻai kūʻai hoʻokūkū maikaʻi loa a me ka maikaʻi maikaʻi, no ka mea, ua ʻoi aku ka maikaʻi o mākou!No laila ʻaʻole ʻoe e kānalua e kelepona iā mākou.
Hoʻopili ʻo ia i ka ʻōlelo "Hoʻopono, hana, ʻoihana, ʻano hou" e hoʻomohala mau i nā huahana hou.Manaʻo ʻo ia i nā mea kūʻai aku, kūleʻa e like me kona kūleʻa ponoʻī.E hooulu kakou i ka lima e hiki mai anaKina Nucleic Acid Extraction Kit (spin column), Hoʻāʻo Nucleic Acid, E hoʻokumu i nā pilina pāʻoihana no ka wā lōʻihi a lanakila-lanakila me kā mākou mea kūʻai a pau, kaʻana like i ka holomua a hauʻoli i ka hauʻoli o ka hoʻolaha ʻana i kā mākou waiwai i ka honua pū.E hilinaʻi iā mākou a e loaʻa hou iā ʻoe.E ʻoluʻolu e kelepona mai iā mākou no ka ʻike hou aku, ke hōʻoiaʻiʻo nei mākou iā ʻoe i ko mākou nānā maikaʻi loa i nā manawa āpau.

Nā kikoʻī

50 Mākaukau, 200 Mākaukau

Hoʻohana ka kit i ke kolamu spin a me ka formula i hoʻomohala ʻia e Foregene, hiki iā ia ke hoʻokaʻawale maikaʻi i ka RNA holoʻokoʻa kiʻekiʻe a kiʻekiʻe mai nā ʻiʻo mea kanu me nā polysaccharides haʻahaʻa a me nā polyphenols.No nā laʻana mea kanu me nā polysaccharides kiʻekiʻe a i ʻole polyphenols, pono e hoʻohana i ka Plant Total RNA Isolation Plus Kit no ka loaʻa ʻana o nā hopena unuhi RNA maikaʻi aʻe.Hāʻawi ka pahu i ke kolamu hoʻomaʻemaʻe DNA e hiki ke wehe maʻalahi i ka DNA genomic mai ka supernatant a me ka lysate kiko.Hiki i ke kolamu RNA-wale ke hoopaa pono i ka RNA.Hiki i ke kit ke hana i ka nui o nā laʻana i ka manawa like.

ʻAʻole i loko o ka ʻōnaehana holoʻokoʻa RNase, no laila ʻaʻole e hoʻohaʻahaʻa ʻia ka RNA i hoʻomaʻemaʻe ʻia.Hiki i ka Buffer PRW1 a me ka Buffer PRW2 ke hōʻoia ʻaʻole i haumia ka RNA i loaʻa e ka protein, DNA, nā ion, a me nā pūhui organik.

Nā ʻāpana pahu

Nā hiʻohiʻona & pono

■ Ka hana ma ka lumi wela (15-25 ℃) ma ke kaʻina holoʻokoʻa, me ka ʻauʻau hau a me ka centrifugation haʻahaʻa wela.
■ Hoʻopiha piha RNase-Free, ʻaʻohe pono e hopohopo e pili ana i ka hōʻino ʻana o RNA.
■ Hoʻopili pono ʻia ke kolamu hoʻomaʻemaʻe DNA i ka DNA, i hiki ai i ka pahu ke hoʻoneʻe i ka ʻino DNA genomic me ka hoʻohui ʻole ʻana i ka DNase.
■ Loaʻa RNA kiʻekiʻe: Hiki i ke kolamu RNA-wale a me ka hoʻohālikelike kū hoʻokahi ke hoʻomaʻemaʻe pono i ka RNA.
■ ʻO ka wikiwiki wikiwiki: maʻalahi e hana a hiki ke hoʻopau i loko o 30 mau minuke.
■ Palekana: ʻaʻole koi ʻia ka reagent organik.
■ Kiʻekiʻe: ʻO nā ʻāpana RNA i hoʻomaʻemaʻe ʻia he maʻemaʻe kiʻekiʻe, ʻaʻohe protein a me nā mea haumia ʻē aʻe, a hiki ke hālāwai me nā noi hoʻokolohua like ʻole.

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA a pau mai nā laʻana o nā mea kanu hou a i ʻole ka hau (ʻoi aku ka ʻiʻo o ka lau mea kanu hou) me ka polysaccharide haʻahaʻa a me ka polyphenol content.

Ka holo hana

Diagram

ʻO ka Plant Total RNA Isolation Kit Plus i hana ʻia he 50mg o nā lau hou o polysaccharides a me polyphenols, a ua hoʻāʻo ʻia ʻo 5% RNA i hoʻomaʻemaʻe ʻia e ka electrophoresis.
1: Maiʻa
2: Ginkgo
3: pulupulu
4: Pomegerane

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 12 mau mahina ma ka lumi wela (15–25 ℃) ma kahi maloʻo, a me 2–8 ℃ no ka manawa lōʻihi (24 mau mahina).

Hiki ke mālama ʻia ʻo Buffer PSL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka 2-hydroxy-1-ethanethiol (koho). Hoʻopili ʻo ia i ka ʻōlelo "Honest, hana, hoʻoikaika, hana hou" e hoʻomohala mau i nā huahana hou.Manaʻo ʻo ia i nā mea kūʻai aku, kūleʻa e like me kona kūleʻa ponoʻī.E hoʻomohala mākou i ka lima maikaʻi i ka wā e hiki mai ana no ka Wholesale Price China Magnetic Beads Method Nucleic Acid Detection Testing Extraction Diagnostic Reagent Bottle Kits, Hiki iā mākou ke hāʻawi iā ʻoe i kekahi o nā kumukūʻai kūʻai hoʻokūkū hoʻokūkū a me ka maikaʻi maikaʻi, no ka mea, ua ʻoi aku ka maikaʻi o mākou!No laila ʻaʻole ʻoe e kānalua e kelepona iā mākou.
ʻO ke kumu kūʻai nui Kina Nucleic Acid Extraction Kit (magnetic beads),Hoʻāʻo Nucleic Acid, E hoʻokumu i nā pilina pāʻoihana no ka wā lōʻihi a lanakila-lanakila me kā mākou mea kūʻai a pau, kaʻana like i ka holomua a hauʻoli i ka hauʻoli o ka hoʻolaha ʻana i kā mākou waiwai i ka honua pū.E hilinaʻi iā mākou a e loaʻa hou iā ʻoe.E ʻoluʻolu e kelepona mai iā mākou no ka ʻike hou aku, ke hōʻoiaʻiʻo nei mākou iā ʻoe i ko mākou nānā maikaʻi loa i nā manawa āpau.